Cargando…

Two-parameter single-molecule analysis for measurement of chromatin mobility

This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-ta...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lerner, Jonathan, Gómez-García, Pablo Aurelio, McCarthy, Ryan L., Liu, Zhe, Lakadamyali, Melike, Zaret, Kenneth S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757679/ https://www.ncbi.nlm.nih.gov/pubmed/33377115 http://dx.doi.org/10.1016/j.xpro.2020.100223

Ejemplares similares

A protocol to quantify chromatin compaction with confocal and super-resolution microscopy in cultured cells
por: Martin, Laura, et al.
Publicado: (2021)

SUPERRESOLUTION IMAGING OF CHROMATIN IN HEALTH AND DISEASE
por: Lakadamyali, Melike
Publicado: (2022)

Different chromatin-scanning modes lead to targeting of compacted chromatin by pioneer factors FOXA1 and SOX2
por: Lerner, Jonathan, et al.
Publicado: (2023)

EU-RNA-seq for in vivo labeling and high throughput sequencing of nascent transcripts
por: Palozola, Katherine C., et al.
Publicado: (2021)

Profiling single-cell chromatin accessibility in plants
por: Marand, Alexandre P., et al.
Publicado: (2021)

Using Single-Molecule Chemo-Mechanical Unfolding to Simultaneously Probe Multiple Structural Parameters in Protein Folding
por: Guinn, Emily J., et al.
Publicado: (2019)

Using single-molecule fluorescence in situ hybridization and immunohistochemistry to count RNA molecules in single cells in zebrafish embryos
por: Keseroglu, Kemal, et al.
Publicado: (2023)

Protocol for bulk and single-nuclei chromatin accessibility quantification in mouse liver tissue
por: Korenfeld, Noga, et al.
Publicado: (2023)

Single-Molecule Techniques to Study Chromatin
por: Chanou, Anna, et al.
Publicado: (2021)

Mesoscale Modeling and Single-Nucleosome Tracking Reveal Remodeling of Clutch Folding and Dynamics in Stem Cell Differentiation
por: Gómez-García, Pablo Aurelio, et al.
Publicado: (2021)

A protocol for simultaneous high-sensitivity genotyping and chromatin accessibility profiling in single cells
por: Turkalj, Sven, et al.
Publicado: (2023)

Protocol for detecting chromatin dynamics and screening chromatin relaxer by FRAP assay
por: Long, Qi, et al.
Publicado: (2021)

InferLoop: leveraging single-cell chromatin accessibility for the signal of chromatin loop
por: Zhang, Feng, et al.
Publicado: (2023)

A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and extension events in cancer cells
por: Lu, Robert, et al.
Publicado: (2022)

Single molecule probing of disordered RNA binding proteins
por: Rhine, Kevin, et al.
Publicado: (2022)

Single-molecule imaging of transcription at damaged chromatin
por: Vítor, Alexandra C., et al.
Publicado: (2019)

Integrating single-cell transcriptomes, chromatin accessibility, and multiomics analysis of mesoderm-induced embryonic stem cells
por: Ko, Kyung Dae, et al.
Publicado: (2023)

Single-Molecule Fluorescence Imaging in Living Saccharomyces cerevisiae Cells
por: Brouwer, Ineke, et al.
Publicado: (2020)

Single-molecule compaction of megabase-long chromatin molecules by multivalent cations
por: Zinchenko, Anatoly, et al.
Publicado: (2018)

The Chromatin Modifier MSK1/2 Suppresses Endocrine Cell Fates during Mouse Pancreatic Development
por: Bhat, Neha, et al.
Publicado: (2016)

Imaging cellular structure across scales with correlated light, superresolution, and electron microscopy
por: Briggs, John A. G., et al.
Publicado: (2012)

Protocol for Single-Molecule Fluorescence In Situ Hybridization for Intact Pancreatic Tissue
por: Farack, Lydia, et al.
Publicado: (2020)

Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with Immunofluorescence
por: Querido, Emmanuelle, et al.
Publicado: (2020)

Protocol for generation and regeneration of PEG-passivated slides for single-molecule measurements
por: Paul, Tapas, et al.
Publicado: (2022)

Protocol for scChaRM-seq: Simultaneous profiling of gene expression, DNA methylation, and chromatin accessibility in single cells
por: Yan, Rui, et al.
Publicado: (2021)

Cross-Talk-Free Multi-Color STORM Imaging Using a Single Fluorophore
por: Tam, Johnny, et al.
Publicado: (2014)

Efficient hemogenic endothelial cell specification by RUNX1 is dependent on baseline chromatin accessibility of RUNX1-regulated TGFβ target genes
por: Howell, Elizabeth D., et al.
Publicado: (2021)

Super-STORM: Molecular Modeling to Achieve Single-molecule Localization with STORM Microscopy
por: Fan, Zhichao, et al.
Publicado: (2020)

Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis
por: Belan, Ondrej, et al.
Publicado: (2021)

Optimized protocol for single-molecule RNA FISH to visualize gene expression in S. cerevisiae
por: Patel, Heta P., et al.
Publicado: (2021)

Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy
por: Ghoneim, Mohamed, et al.
Publicado: (2023)

An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA
por: He, Lingli, et al.
Publicado: (2021)

Chromatin-Bound Proteome Profiling by Genome Capture
por: Aranda, Sergi, et al.
Publicado: (2020)

Super-resolution microscopy reveals how histone tail acetylation affects DNA compaction within nucleosomes in vivo
por: Otterstrom, Jason, et al.
Publicado: (2019)

Optimized lentiviral vector transduction of adherent cells and analysis in sulforhodamine B proliferation and chromatin immunoprecipitation assays
por: Li, Luyuan, et al.
Publicado: (2023)

Ratiometric Single‐Molecule FRET Measurements to Probe Conformational Subpopulations of Intrinsically Disordered Proteins
por: Nasir, Irem, et al.
Publicado: (2020)

Characterization of liquid–liquid phase separation using super-resolution and single-molecule imaging
por: Zhang, Hongchen, et al.
Publicado: (2022)

Protocol for single-molecule pull-down of fluorescently tagged oligomers from cell lysates
por: Djaja, Nathalie, et al.
Publicado: (2023)

Supporting physical activity for mobility in older adults with mobility limitations (SuPA Mobility): study protocol for a randomized controlled trial
por: Rice, Jordyn, et al.
Publicado: (2023)

BIND&MODIFY: a long-range method for single-molecule mapping of chromatin modifications in eukaryotes
por: Weng, Zhe, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_kv6qnf6dhdt9i876ol5c6m8can