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Microfluidics for single-cell lineage tracking over time to characterize transmission of phenotypes in Saccharomyces cerevisiae
The budding yeast Saccharomyces cerevisiae is an excellent model organism to dissect the maintenance and inheritance of phenotypes due to its asymmetric division. This requires following individual cells over time as they go through divisions to define pedigrees. Here, we provide a detailed protocol...
Autores principales: | Bheda, Poonam, Aguilar-Gómez, Diana, Kukhtevich, Igor, Becker, Johannes, Charvin, Gilles, Kirmizis, Antonis, Schneider, Robert |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757727/ https://www.ncbi.nlm.nih.gov/pubmed/33377118 http://dx.doi.org/10.1016/j.xpro.2020.100228 |
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