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A quantitative medium-throughput assay to measure Caenorhabditis elegans development and reproduction

Growth and offspring count are two commonly determined toxicological endpoints for chemical- or gene-induced developmental and reproductive effects in Caenorhabditis elegans. Here, we present a protocol for a 96 h, medium-throughput assay, assessing both endpoints quantitatively within an automated...

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Detalles Bibliográficos
Autores principales: Wittkowski, Paul, Violet, Norman, Oelgeschläger, Michael, Schönfelder, Gilbert, Vogl, Silvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757729/
https://www.ncbi.nlm.nih.gov/pubmed/33377116
http://dx.doi.org/10.1016/j.xpro.2020.100224
Descripción
Sumario:Growth and offspring count are two commonly determined toxicological endpoints for chemical- or gene-induced developmental and reproductive effects in Caenorhabditis elegans. Here, we present a protocol for a 96 h, medium-throughput assay, assessing both endpoints quantitatively within an automated framework using open-source software. The assay utilizes whole 96-well fluorescence images taken with a high-content screening system. Alternatively, conventional fluorescence images can also be utilized with only a few adjustments. For complete details on the use and execution of this protocol, please refer to Wittkowski et al. (2019).