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Orthogonal Blue and Red Light Controlled Cell–Cell Adhesions Enable Sorting-out in Multicellular Structures
[Image: see text] The self-assembly of different cell types into multicellular structures and their organization into spatiotemporally controlled patterns are both challenging and extremely powerful to understand how cells function within tissues and for bottom-up tissue engineering. Here, we not on...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757848/ https://www.ncbi.nlm.nih.gov/pubmed/32610009 http://dx.doi.org/10.1021/acssynbio.0c00150 |
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author | Rasoulinejad, Samaneh Mueller, Marc Nzigou Mombo, Brice Wegner, Seraphine V. |
author_facet | Rasoulinejad, Samaneh Mueller, Marc Nzigou Mombo, Brice Wegner, Seraphine V. |
author_sort | Rasoulinejad, Samaneh |
collection | PubMed |
description | [Image: see text] The self-assembly of different cell types into multicellular structures and their organization into spatiotemporally controlled patterns are both challenging and extremely powerful to understand how cells function within tissues and for bottom-up tissue engineering. Here, we not only independently control the self-assembly of two cell types into multicellular architectures with blue and red light, but also achieve their self-sorting into distinct assemblies. This required developing two cell types that form selective and homophilic cell–cell interactions either under blue or red light using photoswitchable proteins as artificial adhesion molecules. The interactions were individually triggerable with different colors of light, reversible in the dark, and provide noninvasive and temporal control over the cell–cell adhesions. In mixtures of the two cells, each cell type self-assembled independently upon orthogonal photoactivation, and cells sorted out into separate assemblies based on specific self-recognition. These self-sorted multicellular architectures provide us with a powerful tool for producing tissue-like structures from multiple cell types and investigate principles that govern them. |
format | Online Article Text |
id | pubmed-7757848 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-77578482020-12-28 Orthogonal Blue and Red Light Controlled Cell–Cell Adhesions Enable Sorting-out in Multicellular Structures Rasoulinejad, Samaneh Mueller, Marc Nzigou Mombo, Brice Wegner, Seraphine V. ACS Synth Biol [Image: see text] The self-assembly of different cell types into multicellular structures and their organization into spatiotemporally controlled patterns are both challenging and extremely powerful to understand how cells function within tissues and for bottom-up tissue engineering. Here, we not only independently control the self-assembly of two cell types into multicellular architectures with blue and red light, but also achieve their self-sorting into distinct assemblies. This required developing two cell types that form selective and homophilic cell–cell interactions either under blue or red light using photoswitchable proteins as artificial adhesion molecules. The interactions were individually triggerable with different colors of light, reversible in the dark, and provide noninvasive and temporal control over the cell–cell adhesions. In mixtures of the two cells, each cell type self-assembled independently upon orthogonal photoactivation, and cells sorted out into separate assemblies based on specific self-recognition. These self-sorted multicellular architectures provide us with a powerful tool for producing tissue-like structures from multiple cell types and investigate principles that govern them. American Chemical Society 2020-07-01 2020-08-21 /pmc/articles/PMC7757848/ /pubmed/32610009 http://dx.doi.org/10.1021/acssynbio.0c00150 Text en This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
spellingShingle | Rasoulinejad, Samaneh Mueller, Marc Nzigou Mombo, Brice Wegner, Seraphine V. Orthogonal Blue and Red Light Controlled Cell–Cell Adhesions Enable Sorting-out in Multicellular Structures |
title | Orthogonal Blue and Red Light Controlled Cell–Cell
Adhesions Enable Sorting-out in Multicellular Structures |
title_full | Orthogonal Blue and Red Light Controlled Cell–Cell
Adhesions Enable Sorting-out in Multicellular Structures |
title_fullStr | Orthogonal Blue and Red Light Controlled Cell–Cell
Adhesions Enable Sorting-out in Multicellular Structures |
title_full_unstemmed | Orthogonal Blue and Red Light Controlled Cell–Cell
Adhesions Enable Sorting-out in Multicellular Structures |
title_short | Orthogonal Blue and Red Light Controlled Cell–Cell
Adhesions Enable Sorting-out in Multicellular Structures |
title_sort | orthogonal blue and red light controlled cell–cell
adhesions enable sorting-out in multicellular structures |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757848/ https://www.ncbi.nlm.nih.gov/pubmed/32610009 http://dx.doi.org/10.1021/acssynbio.0c00150 |
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