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Highly efficient antibiofilm and antifungal activity of green propolis against Candida species in dentistry materials

This study evaluated the effect of green propolis extract on the adhesion and biofilm formation of Candida species in dentistry materials. Phytochemical analysis of green propolis extract was performed by high-performance liquid chromatography. Adhesion was quantified by counting the number of yeast...

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Autores principales: Bezerra, Carolina Rabelo Falcão, Assunção Borges, Katia Regina, Alves, Rita de Nazaré Silva, Teles, Amanda Mara, Pimentel Rodrigues, Igor Vinicius, da Silva, Marcos Antonio Custódio Neto, Nascimento, Maria do Desterro Soares Brandão, Bezerra, Geusa Felipa de Barros
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757894/
https://www.ncbi.nlm.nih.gov/pubmed/33362254
http://dx.doi.org/10.1371/journal.pone.0228828
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author Bezerra, Carolina Rabelo Falcão
Assunção Borges, Katia Regina
Alves, Rita de Nazaré Silva
Teles, Amanda Mara
Pimentel Rodrigues, Igor Vinicius
da Silva, Marcos Antonio Custódio Neto
Nascimento, Maria do Desterro Soares Brandão
Bezerra, Geusa Felipa de Barros
author_facet Bezerra, Carolina Rabelo Falcão
Assunção Borges, Katia Regina
Alves, Rita de Nazaré Silva
Teles, Amanda Mara
Pimentel Rodrigues, Igor Vinicius
da Silva, Marcos Antonio Custódio Neto
Nascimento, Maria do Desterro Soares Brandão
Bezerra, Geusa Felipa de Barros
author_sort Bezerra, Carolina Rabelo Falcão
collection PubMed
description This study evaluated the effect of green propolis extract on the adhesion and biofilm formation of Candida species in dentistry materials. Phytochemical analysis of green propolis extract was performed by high-performance liquid chromatography. Adhesion was quantified by counting the number of yeast cells adherent to dental material fragments in a Neubauer chamber. Biofilm formation was determined by counting colony-forming units recovered from dental material fragments. The intensity of biofilm adhesion was classified as negative, weak, moderate, strong, or very strong. Fifteen compounds, mainly flavonoids, were identified in green propolis extract. All strains adhered to and formed biofilms on the surfaces of the orthodontic materials studied. On steel and resin, yeast cell adhesion intensities were weak at all incubation times, except for those of Candida parapsilosis and C. tropicalis, which were moderate at 12 h. At 24 and 48 h, C. albicans formed biofilms on steel with moderate adhesion affinities; at 24 and 48 h, C. parapsilosis formed biofilms with very strong affinities. C. tropicalis formed biofilms with strong and very strong affinities at 24 and 48 h, respectively. On resin, all species displayed strong affinity for biofilm formation at 24 and 48 h, except for C. tropicalis, which displayed very strong affinity at only 48 h. Green propolis extract displayed antifungal activity and inhibited both adhesion and biofilm formation at 2.5 μg/mL. This study reinforces the idea that green propolis has antifungal activity and interferes with the virulence of Candida species.
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spelling pubmed-77578942021-01-06 Highly efficient antibiofilm and antifungal activity of green propolis against Candida species in dentistry materials Bezerra, Carolina Rabelo Falcão Assunção Borges, Katia Regina Alves, Rita de Nazaré Silva Teles, Amanda Mara Pimentel Rodrigues, Igor Vinicius da Silva, Marcos Antonio Custódio Neto Nascimento, Maria do Desterro Soares Brandão Bezerra, Geusa Felipa de Barros PLoS One Research Article This study evaluated the effect of green propolis extract on the adhesion and biofilm formation of Candida species in dentistry materials. Phytochemical analysis of green propolis extract was performed by high-performance liquid chromatography. Adhesion was quantified by counting the number of yeast cells adherent to dental material fragments in a Neubauer chamber. Biofilm formation was determined by counting colony-forming units recovered from dental material fragments. The intensity of biofilm adhesion was classified as negative, weak, moderate, strong, or very strong. Fifteen compounds, mainly flavonoids, were identified in green propolis extract. All strains adhered to and formed biofilms on the surfaces of the orthodontic materials studied. On steel and resin, yeast cell adhesion intensities were weak at all incubation times, except for those of Candida parapsilosis and C. tropicalis, which were moderate at 12 h. At 24 and 48 h, C. albicans formed biofilms on steel with moderate adhesion affinities; at 24 and 48 h, C. parapsilosis formed biofilms with very strong affinities. C. tropicalis formed biofilms with strong and very strong affinities at 24 and 48 h, respectively. On resin, all species displayed strong affinity for biofilm formation at 24 and 48 h, except for C. tropicalis, which displayed very strong affinity at only 48 h. Green propolis extract displayed antifungal activity and inhibited both adhesion and biofilm formation at 2.5 μg/mL. This study reinforces the idea that green propolis has antifungal activity and interferes with the virulence of Candida species. Public Library of Science 2020-12-23 /pmc/articles/PMC7757894/ /pubmed/33362254 http://dx.doi.org/10.1371/journal.pone.0228828 Text en © 2020 Bezerra et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bezerra, Carolina Rabelo Falcão
Assunção Borges, Katia Regina
Alves, Rita de Nazaré Silva
Teles, Amanda Mara
Pimentel Rodrigues, Igor Vinicius
da Silva, Marcos Antonio Custódio Neto
Nascimento, Maria do Desterro Soares Brandão
Bezerra, Geusa Felipa de Barros
Highly efficient antibiofilm and antifungal activity of green propolis against Candida species in dentistry materials
title Highly efficient antibiofilm and antifungal activity of green propolis against Candida species in dentistry materials
title_full Highly efficient antibiofilm and antifungal activity of green propolis against Candida species in dentistry materials
title_fullStr Highly efficient antibiofilm and antifungal activity of green propolis against Candida species in dentistry materials
title_full_unstemmed Highly efficient antibiofilm and antifungal activity of green propolis against Candida species in dentistry materials
title_short Highly efficient antibiofilm and antifungal activity of green propolis against Candida species in dentistry materials
title_sort highly efficient antibiofilm and antifungal activity of green propolis against candida species in dentistry materials
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757894/
https://www.ncbi.nlm.nih.gov/pubmed/33362254
http://dx.doi.org/10.1371/journal.pone.0228828
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