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On the emergence of P-Loop NTPase and Rossmann enzymes from a Beta-Alpha-Beta ancestral fragment

This article is dedicated to the memory of Michael G. Rossmann. Dating back to the last universal common ancestor, P-loop NTPases and Rossmanns comprise the most ubiquitous and diverse enzyme lineages. Despite similarities in their overall architecture and phosphate binding motif, a lack of sequence...

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Autores principales: Longo, Liam M, Jabłońska, Jagoda, Vyas, Pratik, Kanade, Manil, Kolodny, Rachel, Ben-Tal, Nir, Tawfik, Dan S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758060/
https://www.ncbi.nlm.nih.gov/pubmed/33295875
http://dx.doi.org/10.7554/eLife.64415
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author Longo, Liam M
Jabłońska, Jagoda
Vyas, Pratik
Kanade, Manil
Kolodny, Rachel
Ben-Tal, Nir
Tawfik, Dan S
author_facet Longo, Liam M
Jabłońska, Jagoda
Vyas, Pratik
Kanade, Manil
Kolodny, Rachel
Ben-Tal, Nir
Tawfik, Dan S
author_sort Longo, Liam M
collection PubMed
description This article is dedicated to the memory of Michael G. Rossmann. Dating back to the last universal common ancestor, P-loop NTPases and Rossmanns comprise the most ubiquitous and diverse enzyme lineages. Despite similarities in their overall architecture and phosphate binding motif, a lack of sequence identity and some fundamental structural differences currently designates them as independent emergences. We systematically searched for structure and sequence elements shared by both lineages. We detected homologous segments that span the first βαβ motif of both lineages, including the phosphate binding loop and a conserved aspartate at the tip of β2. The latter ligates the catalytic metal in P-loop NTPases, while in Rossmanns it binds the nucleotide’s ribose moiety. Tubulin, a Rossmann GTPase, demonstrates the potential of the β2-Asp to take either one of these two roles. While convergence cannot be completely ruled out, we show that both lineages likely emerged from a common βαβ segment that comprises the core of these enzyme families to this very day.
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spelling pubmed-77580602020-12-28 On the emergence of P-Loop NTPase and Rossmann enzymes from a Beta-Alpha-Beta ancestral fragment Longo, Liam M Jabłońska, Jagoda Vyas, Pratik Kanade, Manil Kolodny, Rachel Ben-Tal, Nir Tawfik, Dan S eLife Evolutionary Biology This article is dedicated to the memory of Michael G. Rossmann. Dating back to the last universal common ancestor, P-loop NTPases and Rossmanns comprise the most ubiquitous and diverse enzyme lineages. Despite similarities in their overall architecture and phosphate binding motif, a lack of sequence identity and some fundamental structural differences currently designates them as independent emergences. We systematically searched for structure and sequence elements shared by both lineages. We detected homologous segments that span the first βαβ motif of both lineages, including the phosphate binding loop and a conserved aspartate at the tip of β2. The latter ligates the catalytic metal in P-loop NTPases, while in Rossmanns it binds the nucleotide’s ribose moiety. Tubulin, a Rossmann GTPase, demonstrates the potential of the β2-Asp to take either one of these two roles. While convergence cannot be completely ruled out, we show that both lineages likely emerged from a common βαβ segment that comprises the core of these enzyme families to this very day. eLife Sciences Publications, Ltd 2020-12-09 /pmc/articles/PMC7758060/ /pubmed/33295875 http://dx.doi.org/10.7554/eLife.64415 Text en © 2020, Longo et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Evolutionary Biology
Longo, Liam M
Jabłońska, Jagoda
Vyas, Pratik
Kanade, Manil
Kolodny, Rachel
Ben-Tal, Nir
Tawfik, Dan S
On the emergence of P-Loop NTPase and Rossmann enzymes from a Beta-Alpha-Beta ancestral fragment
title On the emergence of P-Loop NTPase and Rossmann enzymes from a Beta-Alpha-Beta ancestral fragment
title_full On the emergence of P-Loop NTPase and Rossmann enzymes from a Beta-Alpha-Beta ancestral fragment
title_fullStr On the emergence of P-Loop NTPase and Rossmann enzymes from a Beta-Alpha-Beta ancestral fragment
title_full_unstemmed On the emergence of P-Loop NTPase and Rossmann enzymes from a Beta-Alpha-Beta ancestral fragment
title_short On the emergence of P-Loop NTPase and Rossmann enzymes from a Beta-Alpha-Beta ancestral fragment
title_sort on the emergence of p-loop ntpase and rossmann enzymes from a beta-alpha-beta ancestral fragment
topic Evolutionary Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758060/
https://www.ncbi.nlm.nih.gov/pubmed/33295875
http://dx.doi.org/10.7554/eLife.64415
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