Cargando…

Enhanced Chitin Deacetylase Production Ability of Rhodococcus equi CGMCC14861 by Co-culture Fermentation With Staphylococcus sp. MC7

Chitin deacetylase (CDA) can hydrolyze the acetamido group of chitin polymers and its deacetylated derivatives to produce chitosan, an industrially important biopolymer. Compared with traditional chemical methods, biocatalysis by CDA is more environment-friendly and easy to control. However, most re...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Qinyuan, Gao, Xiuzhen, Tu, Linna, Han, Qi, Zhang, Xing, Guo, Yabo, Yan, Wenqin, Shen, Yanbing, Wang, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758288/
https://www.ncbi.nlm.nih.gov/pubmed/33362742
http://dx.doi.org/10.3389/fmicb.2020.592477
_version_ 1783626908917825536
author Ma, Qinyuan
Gao, Xiuzhen
Tu, Linna
Han, Qi
Zhang, Xing
Guo, Yabo
Yan, Wenqin
Shen, Yanbing
Wang, Min
author_facet Ma, Qinyuan
Gao, Xiuzhen
Tu, Linna
Han, Qi
Zhang, Xing
Guo, Yabo
Yan, Wenqin
Shen, Yanbing
Wang, Min
author_sort Ma, Qinyuan
collection PubMed
description Chitin deacetylase (CDA) can hydrolyze the acetamido group of chitin polymers and its deacetylated derivatives to produce chitosan, an industrially important biopolymer. Compared with traditional chemical methods, biocatalysis by CDA is more environment-friendly and easy to control. However, most reported CDA-producing microbial strains show low CDA producing capabilities. Thus, the enhancement of CDA production has always been a challenge. In this study, we report co-culture fermentation to significantly promote the CDA production of Rhodococcus equi CGMCC14861 chitin deacetylase (ReCDA). Due to co-culture fermentation with Staphylococcus sp. MC7, ReCDA yield increased to 21.74 times that of pure culture of R. equi. Additionally, the enhancement was demonstrated to be cell-independent by adding cell-free extracts and the filtrate obtained by 10 kDa ultrafiltration of Staphylococcus sp. MC7. By preliminary characterization, we found extracellular, thermosensitive signal substances produced by Staphylococcus that were less than 10 kDa. We investigated the mechanism of promotion of ReCDA production by transcriptomic analysis. The data showed that 328 genes were upregulated and 1,258 genes were downregulated. The transcription level of the gene encoding ReCDA increased 2.3-fold. These findings provide new insights into the research of co-culture fermentation for the production of CDA and quorum sensing regulation.
format Online
Article
Text
id pubmed-7758288
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-77582882020-12-25 Enhanced Chitin Deacetylase Production Ability of Rhodococcus equi CGMCC14861 by Co-culture Fermentation With Staphylococcus sp. MC7 Ma, Qinyuan Gao, Xiuzhen Tu, Linna Han, Qi Zhang, Xing Guo, Yabo Yan, Wenqin Shen, Yanbing Wang, Min Front Microbiol Microbiology Chitin deacetylase (CDA) can hydrolyze the acetamido group of chitin polymers and its deacetylated derivatives to produce chitosan, an industrially important biopolymer. Compared with traditional chemical methods, biocatalysis by CDA is more environment-friendly and easy to control. However, most reported CDA-producing microbial strains show low CDA producing capabilities. Thus, the enhancement of CDA production has always been a challenge. In this study, we report co-culture fermentation to significantly promote the CDA production of Rhodococcus equi CGMCC14861 chitin deacetylase (ReCDA). Due to co-culture fermentation with Staphylococcus sp. MC7, ReCDA yield increased to 21.74 times that of pure culture of R. equi. Additionally, the enhancement was demonstrated to be cell-independent by adding cell-free extracts and the filtrate obtained by 10 kDa ultrafiltration of Staphylococcus sp. MC7. By preliminary characterization, we found extracellular, thermosensitive signal substances produced by Staphylococcus that were less than 10 kDa. We investigated the mechanism of promotion of ReCDA production by transcriptomic analysis. The data showed that 328 genes were upregulated and 1,258 genes were downregulated. The transcription level of the gene encoding ReCDA increased 2.3-fold. These findings provide new insights into the research of co-culture fermentation for the production of CDA and quorum sensing regulation. Frontiers Media S.A. 2020-12-10 /pmc/articles/PMC7758288/ /pubmed/33362742 http://dx.doi.org/10.3389/fmicb.2020.592477 Text en Copyright © 2020 Ma, Gao, Tu, Han, Zhang, Guo, Yan, Shen and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ma, Qinyuan
Gao, Xiuzhen
Tu, Linna
Han, Qi
Zhang, Xing
Guo, Yabo
Yan, Wenqin
Shen, Yanbing
Wang, Min
Enhanced Chitin Deacetylase Production Ability of Rhodococcus equi CGMCC14861 by Co-culture Fermentation With Staphylococcus sp. MC7
title Enhanced Chitin Deacetylase Production Ability of Rhodococcus equi CGMCC14861 by Co-culture Fermentation With Staphylococcus sp. MC7
title_full Enhanced Chitin Deacetylase Production Ability of Rhodococcus equi CGMCC14861 by Co-culture Fermentation With Staphylococcus sp. MC7
title_fullStr Enhanced Chitin Deacetylase Production Ability of Rhodococcus equi CGMCC14861 by Co-culture Fermentation With Staphylococcus sp. MC7
title_full_unstemmed Enhanced Chitin Deacetylase Production Ability of Rhodococcus equi CGMCC14861 by Co-culture Fermentation With Staphylococcus sp. MC7
title_short Enhanced Chitin Deacetylase Production Ability of Rhodococcus equi CGMCC14861 by Co-culture Fermentation With Staphylococcus sp. MC7
title_sort enhanced chitin deacetylase production ability of rhodococcus equi cgmcc14861 by co-culture fermentation with staphylococcus sp. mc7
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758288/
https://www.ncbi.nlm.nih.gov/pubmed/33362742
http://dx.doi.org/10.3389/fmicb.2020.592477
work_keys_str_mv AT maqinyuan enhancedchitindeacetylaseproductionabilityofrhodococcusequicgmcc14861bycoculturefermentationwithstaphylococcusspmc7
AT gaoxiuzhen enhancedchitindeacetylaseproductionabilityofrhodococcusequicgmcc14861bycoculturefermentationwithstaphylococcusspmc7
AT tulinna enhancedchitindeacetylaseproductionabilityofrhodococcusequicgmcc14861bycoculturefermentationwithstaphylococcusspmc7
AT hanqi enhancedchitindeacetylaseproductionabilityofrhodococcusequicgmcc14861bycoculturefermentationwithstaphylococcusspmc7
AT zhangxing enhancedchitindeacetylaseproductionabilityofrhodococcusequicgmcc14861bycoculturefermentationwithstaphylococcusspmc7
AT guoyabo enhancedchitindeacetylaseproductionabilityofrhodococcusequicgmcc14861bycoculturefermentationwithstaphylococcusspmc7
AT yanwenqin enhancedchitindeacetylaseproductionabilityofrhodococcusequicgmcc14861bycoculturefermentationwithstaphylococcusspmc7
AT shenyanbing enhancedchitindeacetylaseproductionabilityofrhodococcusequicgmcc14861bycoculturefermentationwithstaphylococcusspmc7
AT wangmin enhancedchitindeacetylaseproductionabilityofrhodococcusequicgmcc14861bycoculturefermentationwithstaphylococcusspmc7