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Arbuscular mycorrhizal fungi alter antifungal potential of lemongrass essential oil against Fusarium solani, causing root rot in common bean (Phaseolus vulgaris L.)

The variation in chemical composition of essential oils (EOs) as affected by plant phenology and environmental factors is a crucial limitation in standardization of EOs and thus their effective implementation into novel organic farming. The cultivation of medicinal and aromatic plants (MAP) using bi...

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Autores principales: Eke, Pierre, Adamou, Souleymanou, Fokom, Raymond, Dinango Nya, Vanessa, Tsouh Fokou, Patrick Valere, Nana Wakam, Louise, Nwaga, Dieudonné, Fekam Boyom, Fabrice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758371/
https://www.ncbi.nlm.nih.gov/pubmed/33376819
http://dx.doi.org/10.1016/j.heliyon.2020.e05737
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author Eke, Pierre
Adamou, Souleymanou
Fokom, Raymond
Dinango Nya, Vanessa
Tsouh Fokou, Patrick Valere
Nana Wakam, Louise
Nwaga, Dieudonné
Fekam Boyom, Fabrice
author_facet Eke, Pierre
Adamou, Souleymanou
Fokom, Raymond
Dinango Nya, Vanessa
Tsouh Fokou, Patrick Valere
Nana Wakam, Louise
Nwaga, Dieudonné
Fekam Boyom, Fabrice
author_sort Eke, Pierre
collection PubMed
description The variation in chemical composition of essential oils (EOs) as affected by plant phenology and environmental factors is a crucial limitation in standardization of EOs and thus their effective implementation into novel organic farming. The cultivation of medicinal and aromatic plants (MAP) using bio-elicitors has been advocated as genuine tool to sustainably assure higher quantity and quality EOs. Herein, a field trial and laboratory bioassays were undergone to decipher the impact of a local arbuscular mycorrhizal fungi (AMF) inoculation on agro-morphological yield, EO output and differential suppression of common bean (Phaseolus vulgaris L.) root rot incited by Fusarium solani. The field experiment was laid out following a completely randomized block design (CRBD) with two treatments; non-inoculated control and AMF-inoculation in triplicates. The effects of the EOs were tested against mycelial growth, conidia and common bean seeds germination. Promising concentrations were thereafter assessed for their suppressive effect against Fusariun solani in planta. A significantly higher collar diameter, hypocotyl branching and subsequent Mycorrhizal Dependency (MD) in mycorrhizal lemongrass [(+)AMF] compared to non-inoculated counterpart [(-)AMF]. The resulting EOs consistently repressed F. solani Fs4 conidia germination and mycelium deployment in a dose-dependent manner, with minimum inhibitory concentration (MIC) of 500 ppm for both the EOs, and 125 ppm for the reference fungicide (Ridomil Plus 44 WP). The EO from mycorrhizal lemongrass protected the common bean plantlets from infection by F. solani Fs4 both in laboratory and greenhouse conditions, leading to healthier and vigorous plantlets. The best protection rate was once more obtained with EO from AMF-primed lemongrass at the concentration of 1000 ppm (65.0%) while the lowest disease severity was obtained with Ridomil Plus 44 WP (63.13%). Overall, AMF inoculation shifted the lemongrass biochemical processes with subsequent impact on growth, and an enhanced suppression in Fusarium root rot under greenhouse conditions.
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spelling pubmed-77583712020-12-28 Arbuscular mycorrhizal fungi alter antifungal potential of lemongrass essential oil against Fusarium solani, causing root rot in common bean (Phaseolus vulgaris L.) Eke, Pierre Adamou, Souleymanou Fokom, Raymond Dinango Nya, Vanessa Tsouh Fokou, Patrick Valere Nana Wakam, Louise Nwaga, Dieudonné Fekam Boyom, Fabrice Heliyon Research Article The variation in chemical composition of essential oils (EOs) as affected by plant phenology and environmental factors is a crucial limitation in standardization of EOs and thus their effective implementation into novel organic farming. The cultivation of medicinal and aromatic plants (MAP) using bio-elicitors has been advocated as genuine tool to sustainably assure higher quantity and quality EOs. Herein, a field trial and laboratory bioassays were undergone to decipher the impact of a local arbuscular mycorrhizal fungi (AMF) inoculation on agro-morphological yield, EO output and differential suppression of common bean (Phaseolus vulgaris L.) root rot incited by Fusarium solani. The field experiment was laid out following a completely randomized block design (CRBD) with two treatments; non-inoculated control and AMF-inoculation in triplicates. The effects of the EOs were tested against mycelial growth, conidia and common bean seeds germination. Promising concentrations were thereafter assessed for their suppressive effect against Fusariun solani in planta. A significantly higher collar diameter, hypocotyl branching and subsequent Mycorrhizal Dependency (MD) in mycorrhizal lemongrass [(+)AMF] compared to non-inoculated counterpart [(-)AMF]. The resulting EOs consistently repressed F. solani Fs4 conidia germination and mycelium deployment in a dose-dependent manner, with minimum inhibitory concentration (MIC) of 500 ppm for both the EOs, and 125 ppm for the reference fungicide (Ridomil Plus 44 WP). The EO from mycorrhizal lemongrass protected the common bean plantlets from infection by F. solani Fs4 both in laboratory and greenhouse conditions, leading to healthier and vigorous plantlets. The best protection rate was once more obtained with EO from AMF-primed lemongrass at the concentration of 1000 ppm (65.0%) while the lowest disease severity was obtained with Ridomil Plus 44 WP (63.13%). Overall, AMF inoculation shifted the lemongrass biochemical processes with subsequent impact on growth, and an enhanced suppression in Fusarium root rot under greenhouse conditions. Elsevier 2020-12-19 /pmc/articles/PMC7758371/ /pubmed/33376819 http://dx.doi.org/10.1016/j.heliyon.2020.e05737 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Eke, Pierre
Adamou, Souleymanou
Fokom, Raymond
Dinango Nya, Vanessa
Tsouh Fokou, Patrick Valere
Nana Wakam, Louise
Nwaga, Dieudonné
Fekam Boyom, Fabrice
Arbuscular mycorrhizal fungi alter antifungal potential of lemongrass essential oil against Fusarium solani, causing root rot in common bean (Phaseolus vulgaris L.)
title Arbuscular mycorrhizal fungi alter antifungal potential of lemongrass essential oil against Fusarium solani, causing root rot in common bean (Phaseolus vulgaris L.)
title_full Arbuscular mycorrhizal fungi alter antifungal potential of lemongrass essential oil against Fusarium solani, causing root rot in common bean (Phaseolus vulgaris L.)
title_fullStr Arbuscular mycorrhizal fungi alter antifungal potential of lemongrass essential oil against Fusarium solani, causing root rot in common bean (Phaseolus vulgaris L.)
title_full_unstemmed Arbuscular mycorrhizal fungi alter antifungal potential of lemongrass essential oil against Fusarium solani, causing root rot in common bean (Phaseolus vulgaris L.)
title_short Arbuscular mycorrhizal fungi alter antifungal potential of lemongrass essential oil against Fusarium solani, causing root rot in common bean (Phaseolus vulgaris L.)
title_sort arbuscular mycorrhizal fungi alter antifungal potential of lemongrass essential oil against fusarium solani, causing root rot in common bean (phaseolus vulgaris l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758371/
https://www.ncbi.nlm.nih.gov/pubmed/33376819
http://dx.doi.org/10.1016/j.heliyon.2020.e05737
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