Cargando…

Sural Nerve Perfusion in Mice

Peripheral nerve function is metabolically demanding and nerve energy failure has been implicated in the onset and development of diabetic peripheral neuropathy and neuropathic pain conditions. Distal peripheral nerve oxygen supply relies on the distribution of red blood cells (RBCs) in just a few,...

Descripción completa

Detalles Bibliográficos
Autores principales: Dudele, Anete, Rasmussen, Peter Mondrup, Østergaard, Leif
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758475/
https://www.ncbi.nlm.nih.gov/pubmed/33362454
http://dx.doi.org/10.3389/fnins.2020.579373
_version_ 1783626948174413824
author Dudele, Anete
Rasmussen, Peter Mondrup
Østergaard, Leif
author_facet Dudele, Anete
Rasmussen, Peter Mondrup
Østergaard, Leif
author_sort Dudele, Anete
collection PubMed
description Peripheral nerve function is metabolically demanding and nerve energy failure has been implicated in the onset and development of diabetic peripheral neuropathy and neuropathic pain conditions. Distal peripheral nerve oxygen supply relies on the distribution of red blood cells (RBCs) in just a few, nearby capillary-sized vessels and is therefore technically challenging to characterize. We developed an approach to characterize distal sural nerve hemodynamics in anesthetized, adult male mice using in vivo two-photon laser scanning microscopy. Our results show that RBC velocities in mouse sural nerve vessels are higher than those previously measured in mouse brain, and are sensitive to hindlimb temperatures. Nerve blood flow, measured as RBC flux, however, was similar to that of mouse brain and unaffected by local temperature. Power spectral density analysis of fluctuations in RBC velocities over short time intervals suggest that the technique is sufficiently sensitive and robust to detect subtle flow oscillations over time scales from 0.1 to tens of seconds. We conclude that in vivo two-photon laser scanning microscopy provides a suitable approach to study peripheral nerve hemodynamics in mice, and that local temperature control is important during such measurements.
format Online
Article
Text
id pubmed-7758475
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-77584752020-12-25 Sural Nerve Perfusion in Mice Dudele, Anete Rasmussen, Peter Mondrup Østergaard, Leif Front Neurosci Neuroscience Peripheral nerve function is metabolically demanding and nerve energy failure has been implicated in the onset and development of diabetic peripheral neuropathy and neuropathic pain conditions. Distal peripheral nerve oxygen supply relies on the distribution of red blood cells (RBCs) in just a few, nearby capillary-sized vessels and is therefore technically challenging to characterize. We developed an approach to characterize distal sural nerve hemodynamics in anesthetized, adult male mice using in vivo two-photon laser scanning microscopy. Our results show that RBC velocities in mouse sural nerve vessels are higher than those previously measured in mouse brain, and are sensitive to hindlimb temperatures. Nerve blood flow, measured as RBC flux, however, was similar to that of mouse brain and unaffected by local temperature. Power spectral density analysis of fluctuations in RBC velocities over short time intervals suggest that the technique is sufficiently sensitive and robust to detect subtle flow oscillations over time scales from 0.1 to tens of seconds. We conclude that in vivo two-photon laser scanning microscopy provides a suitable approach to study peripheral nerve hemodynamics in mice, and that local temperature control is important during such measurements. Frontiers Media S.A. 2020-12-10 /pmc/articles/PMC7758475/ /pubmed/33362454 http://dx.doi.org/10.3389/fnins.2020.579373 Text en Copyright © 2020 Dudele, Rasmussen and Østergaard. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Dudele, Anete
Rasmussen, Peter Mondrup
Østergaard, Leif
Sural Nerve Perfusion in Mice
title Sural Nerve Perfusion in Mice
title_full Sural Nerve Perfusion in Mice
title_fullStr Sural Nerve Perfusion in Mice
title_full_unstemmed Sural Nerve Perfusion in Mice
title_short Sural Nerve Perfusion in Mice
title_sort sural nerve perfusion in mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758475/
https://www.ncbi.nlm.nih.gov/pubmed/33362454
http://dx.doi.org/10.3389/fnins.2020.579373
work_keys_str_mv AT dudeleanete suralnerveperfusioninmice
AT rasmussenpetermondrup suralnerveperfusioninmice
AT østergaardleif suralnerveperfusioninmice