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Sural Nerve Perfusion in Mice
Peripheral nerve function is metabolically demanding and nerve energy failure has been implicated in the onset and development of diabetic peripheral neuropathy and neuropathic pain conditions. Distal peripheral nerve oxygen supply relies on the distribution of red blood cells (RBCs) in just a few,...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758475/ https://www.ncbi.nlm.nih.gov/pubmed/33362454 http://dx.doi.org/10.3389/fnins.2020.579373 |
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author | Dudele, Anete Rasmussen, Peter Mondrup Østergaard, Leif |
author_facet | Dudele, Anete Rasmussen, Peter Mondrup Østergaard, Leif |
author_sort | Dudele, Anete |
collection | PubMed |
description | Peripheral nerve function is metabolically demanding and nerve energy failure has been implicated in the onset and development of diabetic peripheral neuropathy and neuropathic pain conditions. Distal peripheral nerve oxygen supply relies on the distribution of red blood cells (RBCs) in just a few, nearby capillary-sized vessels and is therefore technically challenging to characterize. We developed an approach to characterize distal sural nerve hemodynamics in anesthetized, adult male mice using in vivo two-photon laser scanning microscopy. Our results show that RBC velocities in mouse sural nerve vessels are higher than those previously measured in mouse brain, and are sensitive to hindlimb temperatures. Nerve blood flow, measured as RBC flux, however, was similar to that of mouse brain and unaffected by local temperature. Power spectral density analysis of fluctuations in RBC velocities over short time intervals suggest that the technique is sufficiently sensitive and robust to detect subtle flow oscillations over time scales from 0.1 to tens of seconds. We conclude that in vivo two-photon laser scanning microscopy provides a suitable approach to study peripheral nerve hemodynamics in mice, and that local temperature control is important during such measurements. |
format | Online Article Text |
id | pubmed-7758475 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77584752020-12-25 Sural Nerve Perfusion in Mice Dudele, Anete Rasmussen, Peter Mondrup Østergaard, Leif Front Neurosci Neuroscience Peripheral nerve function is metabolically demanding and nerve energy failure has been implicated in the onset and development of diabetic peripheral neuropathy and neuropathic pain conditions. Distal peripheral nerve oxygen supply relies on the distribution of red blood cells (RBCs) in just a few, nearby capillary-sized vessels and is therefore technically challenging to characterize. We developed an approach to characterize distal sural nerve hemodynamics in anesthetized, adult male mice using in vivo two-photon laser scanning microscopy. Our results show that RBC velocities in mouse sural nerve vessels are higher than those previously measured in mouse brain, and are sensitive to hindlimb temperatures. Nerve blood flow, measured as RBC flux, however, was similar to that of mouse brain and unaffected by local temperature. Power spectral density analysis of fluctuations in RBC velocities over short time intervals suggest that the technique is sufficiently sensitive and robust to detect subtle flow oscillations over time scales from 0.1 to tens of seconds. We conclude that in vivo two-photon laser scanning microscopy provides a suitable approach to study peripheral nerve hemodynamics in mice, and that local temperature control is important during such measurements. Frontiers Media S.A. 2020-12-10 /pmc/articles/PMC7758475/ /pubmed/33362454 http://dx.doi.org/10.3389/fnins.2020.579373 Text en Copyright © 2020 Dudele, Rasmussen and Østergaard. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Dudele, Anete Rasmussen, Peter Mondrup Østergaard, Leif Sural Nerve Perfusion in Mice |
title | Sural Nerve Perfusion in Mice |
title_full | Sural Nerve Perfusion in Mice |
title_fullStr | Sural Nerve Perfusion in Mice |
title_full_unstemmed | Sural Nerve Perfusion in Mice |
title_short | Sural Nerve Perfusion in Mice |
title_sort | sural nerve perfusion in mice |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758475/ https://www.ncbi.nlm.nih.gov/pubmed/33362454 http://dx.doi.org/10.3389/fnins.2020.579373 |
work_keys_str_mv | AT dudeleanete suralnerveperfusioninmice AT rasmussenpetermondrup suralnerveperfusioninmice AT østergaardleif suralnerveperfusioninmice |