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Identification of Additive–Epistatic QTLs Conferring Seed Traits in Soybean Using Recombinant Inbred Lines

Seed weight and shape are important agronomic traits that affect soybean quality and yield. In the present study, we used image analysis software to evaluate 100-seed weight and seed shape traits (length, width, perimeter, projection area, length/width, and weight/projection area) of 155 novel recom...

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Autores principales: Li, Meng, Chen, Lingling, Zeng, Jian, Razzaq, Muhammad Khuram, Xu, Xianchao, Xu, Yufei, Wang, Wubin, He, Jianbo, Xing, Guangnan, Gai, Junyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758492/
https://www.ncbi.nlm.nih.gov/pubmed/33362807
http://dx.doi.org/10.3389/fpls.2020.566056
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author Li, Meng
Chen, Lingling
Zeng, Jian
Razzaq, Muhammad Khuram
Xu, Xianchao
Xu, Yufei
Wang, Wubin
He, Jianbo
Xing, Guangnan
Gai, Junyi
author_facet Li, Meng
Chen, Lingling
Zeng, Jian
Razzaq, Muhammad Khuram
Xu, Xianchao
Xu, Yufei
Wang, Wubin
He, Jianbo
Xing, Guangnan
Gai, Junyi
author_sort Li, Meng
collection PubMed
description Seed weight and shape are important agronomic traits that affect soybean quality and yield. In the present study, we used image analysis software to evaluate 100-seed weight and seed shape traits (length, width, perimeter, projection area, length/width, and weight/projection area) of 155 novel recombinant inbred soybean lines (NJRISX) generated by crossing “Su88-M21” and “XYXHD”. We examined quantitative trait loci (QTLs) associated with the six traits (except seed weight per projection area), and identified 42 additive QTLs (5–8 QTLs per trait) accounting for 24.9–37.5% of the phenotypic variation (PV). Meanwhile, 2–4 epistatic QTL pairs per trait out of a total of 18 accounted for 2.5–7.2% of the PV; and unmapped minor QTLs accounted for the remaining 35.0–56.7% of the PV. A total of 28 additive and 11 epistatic QTL pairs were concentrated in nine joint QTL segments (JQSs), indicating that QTLs associated with seed weight and shape are closely related and interacted. An interaction was also detected between additive and epistatic QTL pairs and environment, which made significant contributions of 1.4–9.5% and 0.4–0.8% to the PV, respectively. We annotated 18 candidate genes in the nine JQSs, which were important for interpreting the close relationships among the six traits. These findings indicate that examining the interactions between closely related traits rather than only analyzing individual trait provides more useful insight into the genetic system of the interrelated traits for which there has been limited QTL information.
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spelling pubmed-77584922020-12-25 Identification of Additive–Epistatic QTLs Conferring Seed Traits in Soybean Using Recombinant Inbred Lines Li, Meng Chen, Lingling Zeng, Jian Razzaq, Muhammad Khuram Xu, Xianchao Xu, Yufei Wang, Wubin He, Jianbo Xing, Guangnan Gai, Junyi Front Plant Sci Plant Science Seed weight and shape are important agronomic traits that affect soybean quality and yield. In the present study, we used image analysis software to evaluate 100-seed weight and seed shape traits (length, width, perimeter, projection area, length/width, and weight/projection area) of 155 novel recombinant inbred soybean lines (NJRISX) generated by crossing “Su88-M21” and “XYXHD”. We examined quantitative trait loci (QTLs) associated with the six traits (except seed weight per projection area), and identified 42 additive QTLs (5–8 QTLs per trait) accounting for 24.9–37.5% of the phenotypic variation (PV). Meanwhile, 2–4 epistatic QTL pairs per trait out of a total of 18 accounted for 2.5–7.2% of the PV; and unmapped minor QTLs accounted for the remaining 35.0–56.7% of the PV. A total of 28 additive and 11 epistatic QTL pairs were concentrated in nine joint QTL segments (JQSs), indicating that QTLs associated with seed weight and shape are closely related and interacted. An interaction was also detected between additive and epistatic QTL pairs and environment, which made significant contributions of 1.4–9.5% and 0.4–0.8% to the PV, respectively. We annotated 18 candidate genes in the nine JQSs, which were important for interpreting the close relationships among the six traits. These findings indicate that examining the interactions between closely related traits rather than only analyzing individual trait provides more useful insight into the genetic system of the interrelated traits for which there has been limited QTL information. Frontiers Media S.A. 2020-12-10 /pmc/articles/PMC7758492/ /pubmed/33362807 http://dx.doi.org/10.3389/fpls.2020.566056 Text en Copyright © 2020 Li, Chen, Zeng, Razzaq, Xu, Xu, Wang, He, Xing and Gai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Meng
Chen, Lingling
Zeng, Jian
Razzaq, Muhammad Khuram
Xu, Xianchao
Xu, Yufei
Wang, Wubin
He, Jianbo
Xing, Guangnan
Gai, Junyi
Identification of Additive–Epistatic QTLs Conferring Seed Traits in Soybean Using Recombinant Inbred Lines
title Identification of Additive–Epistatic QTLs Conferring Seed Traits in Soybean Using Recombinant Inbred Lines
title_full Identification of Additive–Epistatic QTLs Conferring Seed Traits in Soybean Using Recombinant Inbred Lines
title_fullStr Identification of Additive–Epistatic QTLs Conferring Seed Traits in Soybean Using Recombinant Inbred Lines
title_full_unstemmed Identification of Additive–Epistatic QTLs Conferring Seed Traits in Soybean Using Recombinant Inbred Lines
title_short Identification of Additive–Epistatic QTLs Conferring Seed Traits in Soybean Using Recombinant Inbred Lines
title_sort identification of additive–epistatic qtls conferring seed traits in soybean using recombinant inbred lines
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758492/
https://www.ncbi.nlm.nih.gov/pubmed/33362807
http://dx.doi.org/10.3389/fpls.2020.566056
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