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Pharmacological Characterization of the Stick Insect Carausius morosus Allatostatin-C Receptor with Its Endogenous Agonist

[Image: see text] G protein-coupled receptors (GPCRs) play a pivotal role in regulating key physiological events in all animal species. Recent advances in collective analysis of genes and proteins revealed numerous potential neuropeptides and GPCRs from insect species, allowing for the characterizat...

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Autores principales: Işbilir, Ali, Duan Sahbaz, Burcin, Tuncgenc, Gunes, Bünemann, Moritz, Lohse, Martin J., Birgül-Iyison, Necla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758886/
https://www.ncbi.nlm.nih.gov/pubmed/33376856
http://dx.doi.org/10.1021/acsomega.0c03382
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author Işbilir, Ali
Duan Sahbaz, Burcin
Tuncgenc, Gunes
Bünemann, Moritz
Lohse, Martin J.
Birgül-Iyison, Necla
author_facet Işbilir, Ali
Duan Sahbaz, Burcin
Tuncgenc, Gunes
Bünemann, Moritz
Lohse, Martin J.
Birgül-Iyison, Necla
author_sort Işbilir, Ali
collection PubMed
description [Image: see text] G protein-coupled receptors (GPCRs) play a pivotal role in regulating key physiological events in all animal species. Recent advances in collective analysis of genes and proteins revealed numerous potential neuropeptides and GPCRs from insect species, allowing for the characterization of peptide–receptor pairs. In this work, we used fluorescence resonance energy transfer (FRET)-based genetically encoded biosensors in intact mammalian cells to study the pharmacological features of the cognate GPCR of the type-C allatostatin (AST-C) peptide from the stick insect, Carausius morosus. Analysis of multiple downstream pathways revealed that AST-C can activate the human Gi(2) protein, and not Gs or Gq, through AST-C receptor (AlstRC). Activated AlstRC recruits β-arrestin2 independent of the Gi protein but stimulates ERK phosphorylation in a Gi protein-dependent manner. Identification of Gαi-, arrestin-, and GRK-like transcripts from C. morosus revealed high evolutionary conservation at the G protein level, while β-arrestins and GRKs displayed less conservation. In conclusion, our study provides experimental and homology-based evidence on the functionality of vertebrate G proteins and downstream signaling biosensors to characterize early signaling steps of an insect GPCR. These results may serve as a scaffold for developing assays to characterize pharmacological and structural aspects of other insect GPCRs and can be used in deorphanization and pesticide studies.
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spelling pubmed-77588862020-12-28 Pharmacological Characterization of the Stick Insect Carausius morosus Allatostatin-C Receptor with Its Endogenous Agonist Işbilir, Ali Duan Sahbaz, Burcin Tuncgenc, Gunes Bünemann, Moritz Lohse, Martin J. Birgül-Iyison, Necla ACS Omega [Image: see text] G protein-coupled receptors (GPCRs) play a pivotal role in regulating key physiological events in all animal species. Recent advances in collective analysis of genes and proteins revealed numerous potential neuropeptides and GPCRs from insect species, allowing for the characterization of peptide–receptor pairs. In this work, we used fluorescence resonance energy transfer (FRET)-based genetically encoded biosensors in intact mammalian cells to study the pharmacological features of the cognate GPCR of the type-C allatostatin (AST-C) peptide from the stick insect, Carausius morosus. Analysis of multiple downstream pathways revealed that AST-C can activate the human Gi(2) protein, and not Gs or Gq, through AST-C receptor (AlstRC). Activated AlstRC recruits β-arrestin2 independent of the Gi protein but stimulates ERK phosphorylation in a Gi protein-dependent manner. Identification of Gαi-, arrestin-, and GRK-like transcripts from C. morosus revealed high evolutionary conservation at the G protein level, while β-arrestins and GRKs displayed less conservation. In conclusion, our study provides experimental and homology-based evidence on the functionality of vertebrate G proteins and downstream signaling biosensors to characterize early signaling steps of an insect GPCR. These results may serve as a scaffold for developing assays to characterize pharmacological and structural aspects of other insect GPCRs and can be used in deorphanization and pesticide studies. American Chemical Society 2020-12-08 /pmc/articles/PMC7758886/ /pubmed/33376856 http://dx.doi.org/10.1021/acsomega.0c03382 Text en © 2020 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Işbilir, Ali
Duan Sahbaz, Burcin
Tuncgenc, Gunes
Bünemann, Moritz
Lohse, Martin J.
Birgül-Iyison, Necla
Pharmacological Characterization of the Stick Insect Carausius morosus Allatostatin-C Receptor with Its Endogenous Agonist
title Pharmacological Characterization of the Stick Insect Carausius morosus Allatostatin-C Receptor with Its Endogenous Agonist
title_full Pharmacological Characterization of the Stick Insect Carausius morosus Allatostatin-C Receptor with Its Endogenous Agonist
title_fullStr Pharmacological Characterization of the Stick Insect Carausius morosus Allatostatin-C Receptor with Its Endogenous Agonist
title_full_unstemmed Pharmacological Characterization of the Stick Insect Carausius morosus Allatostatin-C Receptor with Its Endogenous Agonist
title_short Pharmacological Characterization of the Stick Insect Carausius morosus Allatostatin-C Receptor with Its Endogenous Agonist
title_sort pharmacological characterization of the stick insect carausius morosus allatostatin-c receptor with its endogenous agonist
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758886/
https://www.ncbi.nlm.nih.gov/pubmed/33376856
http://dx.doi.org/10.1021/acsomega.0c03382
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