Dnajb8, a target gene of SOX30, is dispensable for male fertility in mice

BACKGROUND: The DNAJ family of molecular chaperones maintains protein homeostasis in mitotic and postmeiotic cells, especially germ cells. Recently, we found that the transcription factor SOX30 initiates transcription of Dnajb8 during late meiosis and spermiogenesis in mouse testes. METHODS: We used...

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Autores principales: Wang, Fengsong, Kong, Shuai, Hu, Xuechun, Li, Xin, Xu, Bo, Yue, Qiuling, Fu, Kaiqiang, Ye, Lan, Bai, Shun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759119/
https://www.ncbi.nlm.nih.gov/pubmed/33391882
http://dx.doi.org/10.7717/peerj.10582
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author Wang, Fengsong
Kong, Shuai
Hu, Xuechun
Li, Xin
Xu, Bo
Yue, Qiuling
Fu, Kaiqiang
Ye, Lan
Bai, Shun
author_facet Wang, Fengsong
Kong, Shuai
Hu, Xuechun
Li, Xin
Xu, Bo
Yue, Qiuling
Fu, Kaiqiang
Ye, Lan
Bai, Shun
author_sort Wang, Fengsong
collection PubMed
description BACKGROUND: The DNAJ family of molecular chaperones maintains protein homeostasis in mitotic and postmeiotic cells, especially germ cells. Recently, we found that the transcription factor SOX30 initiates transcription of Dnajb8 during late meiosis and spermiogenesis in mouse testes. METHODS: We used the CRISPR/Cas9 system to generate Dnajb8 mutant mice and analyze the phenotype of the Dnajb8 mutants. RESULTS: AlthoughDnajb8 is an evolutionarily conserved gene, it is not essential for spermatogenesis and male fertility. We provide this phenotypic information, which could prevent duplicative work by other groups.
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spelling pubmed-77591192020-12-31 Dnajb8, a target gene of SOX30, is dispensable for male fertility in mice Wang, Fengsong Kong, Shuai Hu, Xuechun Li, Xin Xu, Bo Yue, Qiuling Fu, Kaiqiang Ye, Lan Bai, Shun PeerJ Developmental Biology BACKGROUND: The DNAJ family of molecular chaperones maintains protein homeostasis in mitotic and postmeiotic cells, especially germ cells. Recently, we found that the transcription factor SOX30 initiates transcription of Dnajb8 during late meiosis and spermiogenesis in mouse testes. METHODS: We used the CRISPR/Cas9 system to generate Dnajb8 mutant mice and analyze the phenotype of the Dnajb8 mutants. RESULTS: AlthoughDnajb8 is an evolutionarily conserved gene, it is not essential for spermatogenesis and male fertility. We provide this phenotypic information, which could prevent duplicative work by other groups. PeerJ Inc. 2020-12-21 /pmc/articles/PMC7759119/ /pubmed/33391882 http://dx.doi.org/10.7717/peerj.10582 Text en ©2020 Wang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Developmental Biology
Wang, Fengsong
Kong, Shuai
Hu, Xuechun
Li, Xin
Xu, Bo
Yue, Qiuling
Fu, Kaiqiang
Ye, Lan
Bai, Shun
Dnajb8, a target gene of SOX30, is dispensable for male fertility in mice
title Dnajb8, a target gene of SOX30, is dispensable for male fertility in mice
title_full Dnajb8, a target gene of SOX30, is dispensable for male fertility in mice
title_fullStr Dnajb8, a target gene of SOX30, is dispensable for male fertility in mice
title_full_unstemmed Dnajb8, a target gene of SOX30, is dispensable for male fertility in mice
title_short Dnajb8, a target gene of SOX30, is dispensable for male fertility in mice
title_sort dnajb8, a target gene of sox30, is dispensable for male fertility in mice
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759119/
https://www.ncbi.nlm.nih.gov/pubmed/33391882
http://dx.doi.org/10.7717/peerj.10582
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