Cargando…

A Novel Bioreactor System Capable of Simulating the In Vivo Conditions of Synovial Joints

Any significant in vitro evaluation of cartilage tissue engineering and cartilage repair strategies has to be performed under the harsh conditions encountered in vivo within synovial joints. To this end, we have developed a novel automated physiological robot reactor system (PRRS) that is capable of...

Descripción completa

Detalles Bibliográficos
Autores principales: Tekari, Adel, Egli, Rainer J., Schmid, Veit, Justiz, Joern, Luginbuehl, Reto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759289/
https://www.ncbi.nlm.nih.gov/pubmed/33267725
http://dx.doi.org/10.1089/ten.tec.2020.0161
_version_ 1783627092420722688
author Tekari, Adel
Egli, Rainer J.
Schmid, Veit
Justiz, Joern
Luginbuehl, Reto
author_facet Tekari, Adel
Egli, Rainer J.
Schmid, Veit
Justiz, Joern
Luginbuehl, Reto
author_sort Tekari, Adel
collection PubMed
description Any significant in vitro evaluation of cartilage tissue engineering and cartilage repair strategies has to be performed under the harsh conditions encountered in vivo within synovial joints. To this end, we have developed a novel automated physiological robot reactor system (PRRS) that is capable of recapitulating complex physiological motions and load patterns within an environment similar to that found in the human knee. The PRRS consists of a mechanical stimulation unit (MSU) and an automatic sample changer (ASC) within an environment control box in which the humidity, temperature, and gas composition are tightly regulated. The MSU has three linear (orthogonal) axes and one rotational degree of freedom (around the z-axis). The ASC provides space for up to 24 samples, which can be allocated to individual stimulation patterns. Cell-seeded scaffolds and ex vivo tissue culture systems were established to demonstrate the applicability of the PRRS to the investigation of the effect of load and environmental conditions on engineering and maintenance of articular cartilage in vitro. The bioreactor is a flexible system that has the potential to be applied for culturing connective tissues other than cartilage, such as bone and intervertebral disc tissue, even though the mechanical and environmental parameters are very different. IMPACT STATEMENT: The success of engineered cartilage tissues depends on the biological/biochemical stimulations parameters, which should be as close as possible to the conditions observed in vivo. The design of bioreactors should be, therefore, inspired from the in vivo conditions, rather than the application of one or two degree of freedom loading cycles.
format Online
Article
Text
id pubmed-7759289
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Mary Ann Liebert, Inc., publishers
record_format MEDLINE/PubMed
spelling pubmed-77592892020-12-28 A Novel Bioreactor System Capable of Simulating the In Vivo Conditions of Synovial Joints Tekari, Adel Egli, Rainer J. Schmid, Veit Justiz, Joern Luginbuehl, Reto Tissue Eng Part C Methods Methods Articles Any significant in vitro evaluation of cartilage tissue engineering and cartilage repair strategies has to be performed under the harsh conditions encountered in vivo within synovial joints. To this end, we have developed a novel automated physiological robot reactor system (PRRS) that is capable of recapitulating complex physiological motions and load patterns within an environment similar to that found in the human knee. The PRRS consists of a mechanical stimulation unit (MSU) and an automatic sample changer (ASC) within an environment control box in which the humidity, temperature, and gas composition are tightly regulated. The MSU has three linear (orthogonal) axes and one rotational degree of freedom (around the z-axis). The ASC provides space for up to 24 samples, which can be allocated to individual stimulation patterns. Cell-seeded scaffolds and ex vivo tissue culture systems were established to demonstrate the applicability of the PRRS to the investigation of the effect of load and environmental conditions on engineering and maintenance of articular cartilage in vitro. The bioreactor is a flexible system that has the potential to be applied for culturing connective tissues other than cartilage, such as bone and intervertebral disc tissue, even though the mechanical and environmental parameters are very different. IMPACT STATEMENT: The success of engineered cartilage tissues depends on the biological/biochemical stimulations parameters, which should be as close as possible to the conditions observed in vivo. The design of bioreactors should be, therefore, inspired from the in vivo conditions, rather than the application of one or two degree of freedom loading cycles. Mary Ann Liebert, Inc., publishers 2020-12-01 2020-12-16 /pmc/articles/PMC7759289/ /pubmed/33267725 http://dx.doi.org/10.1089/ten.tec.2020.0161 Text en © Adel Tekari, et al., 2020; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methods Articles
Tekari, Adel
Egli, Rainer J.
Schmid, Veit
Justiz, Joern
Luginbuehl, Reto
A Novel Bioreactor System Capable of Simulating the In Vivo Conditions of Synovial Joints
title A Novel Bioreactor System Capable of Simulating the In Vivo Conditions of Synovial Joints
title_full A Novel Bioreactor System Capable of Simulating the In Vivo Conditions of Synovial Joints
title_fullStr A Novel Bioreactor System Capable of Simulating the In Vivo Conditions of Synovial Joints
title_full_unstemmed A Novel Bioreactor System Capable of Simulating the In Vivo Conditions of Synovial Joints
title_short A Novel Bioreactor System Capable of Simulating the In Vivo Conditions of Synovial Joints
title_sort novel bioreactor system capable of simulating the in vivo conditions of synovial joints
topic Methods Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759289/
https://www.ncbi.nlm.nih.gov/pubmed/33267725
http://dx.doi.org/10.1089/ten.tec.2020.0161
work_keys_str_mv AT tekariadel anovelbioreactorsystemcapableofsimulatingtheinvivoconditionsofsynovialjoints
AT eglirainerj anovelbioreactorsystemcapableofsimulatingtheinvivoconditionsofsynovialjoints
AT schmidveit anovelbioreactorsystemcapableofsimulatingtheinvivoconditionsofsynovialjoints
AT justizjoern anovelbioreactorsystemcapableofsimulatingtheinvivoconditionsofsynovialjoints
AT luginbuehlreto anovelbioreactorsystemcapableofsimulatingtheinvivoconditionsofsynovialjoints
AT tekariadel novelbioreactorsystemcapableofsimulatingtheinvivoconditionsofsynovialjoints
AT eglirainerj novelbioreactorsystemcapableofsimulatingtheinvivoconditionsofsynovialjoints
AT schmidveit novelbioreactorsystemcapableofsimulatingtheinvivoconditionsofsynovialjoints
AT justizjoern novelbioreactorsystemcapableofsimulatingtheinvivoconditionsofsynovialjoints
AT luginbuehlreto novelbioreactorsystemcapableofsimulatingtheinvivoconditionsofsynovialjoints