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Magnesium in Combinatorial With Valproic Acid Suppressed the Proliferation and Migration of Human Bladder Cancer Cells
Magnesium, the second most predominant intracellular cation, plays a crucial role in many physiological functions; magnesium-based biomaterials have been widely used in clinical application. In a variety of cancer types, the high intracellular concentration of magnesium contributes to cancer initiat...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759627/ https://www.ncbi.nlm.nih.gov/pubmed/33363019 http://dx.doi.org/10.3389/fonc.2020.589112 |
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author | Li, Tianye Yu, Yang Shi, Hang Cao, Yuhua Liu, Xiangfu Hao, Zhenzhen Ren, Yuping Qin, Gaowu Huang, Yongye Wang, Bing |
author_facet | Li, Tianye Yu, Yang Shi, Hang Cao, Yuhua Liu, Xiangfu Hao, Zhenzhen Ren, Yuping Qin, Gaowu Huang, Yongye Wang, Bing |
author_sort | Li, Tianye |
collection | PubMed |
description | Magnesium, the second most predominant intracellular cation, plays a crucial role in many physiological functions; magnesium-based biomaterials have been widely used in clinical application. In a variety of cancer types, the high intracellular concentration of magnesium contributes to cancer initiation and progression. Therefore, we initiated this study to investigate the likelihood of confounding magnesium with cancer therapy. In this study, the anti-tumor activity of magnesium and underlying mechanisms were assessed in bladder cancer both in vitro and in vivo. The results indicated that the proliferation of bladder cancer cells was inhibited by treatment with a high concentration of MgCl(2) or MgSO(4). The apoptosis, G0/G1 cell cycle arrest, autophagy, and ER stress were promoted following treatment with MgCl(2). However, the migratory ability of MgCl(2) treated cells was similar to that of control cells, as revealed by the trans-well assay. Besides, no significant difference was observed in the proportion of CD44 or CD133 positive cells between the control and MgCl(2) treated cells. Thus, to improve the therapeutic effect of magnesium, VPA was used to treat cancer cells in combination with MgCl(2). As expected, combination treatment with MgCl(2) and VPA could markedly reduce proliferation, migration, and in vivo tumorigenicity of UC3 cells. Moreover, the Wnt signaling was down-regulated, and ERK signaling was activated in the cells treated with combination treatment. In conclusion, the accurate utilization of MgCl(2) in targeting autophagy might be beneficial in cancer therapy. Although further studies are warranted, the combination treatment of MgCl(2) with VPA is an effective strategy to improve the outcome of chemotherapy. |
format | Online Article Text |
id | pubmed-7759627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77596272020-12-26 Magnesium in Combinatorial With Valproic Acid Suppressed the Proliferation and Migration of Human Bladder Cancer Cells Li, Tianye Yu, Yang Shi, Hang Cao, Yuhua Liu, Xiangfu Hao, Zhenzhen Ren, Yuping Qin, Gaowu Huang, Yongye Wang, Bing Front Oncol Oncology Magnesium, the second most predominant intracellular cation, plays a crucial role in many physiological functions; magnesium-based biomaterials have been widely used in clinical application. In a variety of cancer types, the high intracellular concentration of magnesium contributes to cancer initiation and progression. Therefore, we initiated this study to investigate the likelihood of confounding magnesium with cancer therapy. In this study, the anti-tumor activity of magnesium and underlying mechanisms were assessed in bladder cancer both in vitro and in vivo. The results indicated that the proliferation of bladder cancer cells was inhibited by treatment with a high concentration of MgCl(2) or MgSO(4). The apoptosis, G0/G1 cell cycle arrest, autophagy, and ER stress were promoted following treatment with MgCl(2). However, the migratory ability of MgCl(2) treated cells was similar to that of control cells, as revealed by the trans-well assay. Besides, no significant difference was observed in the proportion of CD44 or CD133 positive cells between the control and MgCl(2) treated cells. Thus, to improve the therapeutic effect of magnesium, VPA was used to treat cancer cells in combination with MgCl(2). As expected, combination treatment with MgCl(2) and VPA could markedly reduce proliferation, migration, and in vivo tumorigenicity of UC3 cells. Moreover, the Wnt signaling was down-regulated, and ERK signaling was activated in the cells treated with combination treatment. In conclusion, the accurate utilization of MgCl(2) in targeting autophagy might be beneficial in cancer therapy. Although further studies are warranted, the combination treatment of MgCl(2) with VPA is an effective strategy to improve the outcome of chemotherapy. Frontiers Media S.A. 2020-12-11 /pmc/articles/PMC7759627/ /pubmed/33363019 http://dx.doi.org/10.3389/fonc.2020.589112 Text en Copyright © 2020 Li, Yu, Shi, Cao, Liu, Hao, Ren, Qin, Huang and Wang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Li, Tianye Yu, Yang Shi, Hang Cao, Yuhua Liu, Xiangfu Hao, Zhenzhen Ren, Yuping Qin, Gaowu Huang, Yongye Wang, Bing Magnesium in Combinatorial With Valproic Acid Suppressed the Proliferation and Migration of Human Bladder Cancer Cells |
title | Magnesium in Combinatorial With Valproic Acid Suppressed the Proliferation and Migration of Human Bladder Cancer Cells |
title_full | Magnesium in Combinatorial With Valproic Acid Suppressed the Proliferation and Migration of Human Bladder Cancer Cells |
title_fullStr | Magnesium in Combinatorial With Valproic Acid Suppressed the Proliferation and Migration of Human Bladder Cancer Cells |
title_full_unstemmed | Magnesium in Combinatorial With Valproic Acid Suppressed the Proliferation and Migration of Human Bladder Cancer Cells |
title_short | Magnesium in Combinatorial With Valproic Acid Suppressed the Proliferation and Migration of Human Bladder Cancer Cells |
title_sort | magnesium in combinatorial with valproic acid suppressed the proliferation and migration of human bladder cancer cells |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759627/ https://www.ncbi.nlm.nih.gov/pubmed/33363019 http://dx.doi.org/10.3389/fonc.2020.589112 |
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