Triatoma rosai sp. nov. (Hemiptera, Triatominae): A New Species of Argentinian Chagas Disease Vector Described Based on Integrative Taxonomy

SIMPLE SUMMARY: Although all triatomines are potential vectors of Chagas' disease, there are species with greater or lesser vectorial importance. Therefore, the correct identification of triatomines species is essential for the vector control programs. In general, triatomines are identified by external morphological characters. However, some species are very similar or even morphologically identical, being important the use of complementary analyses for the correct identification of species. For this reason, this study focused on the use of morphological, morphometric, molecular data, and experimental crosses to describe Triatoma rosai sp. nov., a new species of Argentinian Chagas disease vector. Significant morphological and morphometric differences, associated with phylogenetic support and high mortality rate of the hybrids made it possible to confirm the specific status of T. rosai sp. nov., emphasizing the importance of integrative analyses for the taxonomy of triatomines. ABSTRACT: Chagas disease is the most prevalent neglected tropical disease in the Americas and makes an important contribution to morbidity and mortality rates in countries where it is endemic since 30 to 40% of patients develop cardiac diseases, gastrointestinal disorders, or both. In this paper, a new species of the genus Triatoma is described based on specimens collected in the Department San Miguel, Province of Corrientes, Argentina. Triatoma rosai sp. nov. is closely related to T. sordida (Stål, 1859), and was characterized based on integrative taxonomy using morphological, morphometric, molecular data, and experimental crosses. These analyses, combined with data from the literature (cytogenetics, electrophoresis pattern, molecular analyses, cuticular hydrocarbons pattern, geometric morphometry, cycle, and average time of life as well as geographic distribution) confirm the specific status of T. rosai sp. nov. Natural Trypanosoma cruzi infection, coupled with its presence mostly in peridomestic habitats, indicates that this species can be considered as an important Chagas disease vector from Argentina.