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Sensing of Proteins by ICD Response of Iron(II) Clathrochelates Functionalized by Carboxyalkylsulfide Groups

Recognition of elements of protein tertiary structure is crucial for biotechnological and biomedical tasks; this makes the development of optical sensors for certain protein surface elements important. Herein, we demonstrated the ability of iron(II) clathrochelates (1–3) functionalized with mono-, d...

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Autores principales: Losytskyy, Mykhaylo, Chornenka, Nina, Vakarov, Serhii, Meier-Menches, Samuel M., Gerner, Christopher, Potocki, Slawomir, Arion, Vladimir B., Gumienna-Kontecka, Elzbieta, Voloshin, Yan, Kovalska, Vladyslava
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759900/
https://www.ncbi.nlm.nih.gov/pubmed/33256144
http://dx.doi.org/10.3390/biom10121602
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author Losytskyy, Mykhaylo
Chornenka, Nina
Vakarov, Serhii
Meier-Menches, Samuel M.
Gerner, Christopher
Potocki, Slawomir
Arion, Vladimir B.
Gumienna-Kontecka, Elzbieta
Voloshin, Yan
Kovalska, Vladyslava
author_facet Losytskyy, Mykhaylo
Chornenka, Nina
Vakarov, Serhii
Meier-Menches, Samuel M.
Gerner, Christopher
Potocki, Slawomir
Arion, Vladimir B.
Gumienna-Kontecka, Elzbieta
Voloshin, Yan
Kovalska, Vladyslava
author_sort Losytskyy, Mykhaylo
collection PubMed
description Recognition of elements of protein tertiary structure is crucial for biotechnological and biomedical tasks; this makes the development of optical sensors for certain protein surface elements important. Herein, we demonstrated the ability of iron(II) clathrochelates (1–3) functionalized with mono-, di- and hexa-carboxyalkylsulfide to induce selective circular dichroism (CD) response upon binding to globular proteins. Thus, inherently CD-silent clathrochelates revealed selective inducing of CD spectra when binding to human serum albumin (HSA) (1, 2), beta-lactoglobuline (2) and bovine serum albumin (BSA) (3). Hence, functionalization of iron(II) clathrochelates with the carboxyalkylsulfide group appears to be a promising tool for the design of CD-probes sensitive to certain surface elements of proteins tertiary structure. Additionally, interaction of 1–3 with proteins was also studied by isothermal titration calorimetry, protein fluorescence quenching, electrospray ionization mass spectrometry (ESI-MS) and computer simulations. Formation of both 1:1 and 1:2 assemblies of HSA with 1–3 was evidenced by ESI-MS. A protein fluorescence quenching study suggests that 3 binds with both BSA and HSA via the sites close to Trp residues. Molecular docking calculations indicate that for both BSA and HSA, binding of 3 to Site I and to an “additional site” is more favorable energetically than binding to Site II.
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spelling pubmed-77599002020-12-26 Sensing of Proteins by ICD Response of Iron(II) Clathrochelates Functionalized by Carboxyalkylsulfide Groups Losytskyy, Mykhaylo Chornenka, Nina Vakarov, Serhii Meier-Menches, Samuel M. Gerner, Christopher Potocki, Slawomir Arion, Vladimir B. Gumienna-Kontecka, Elzbieta Voloshin, Yan Kovalska, Vladyslava Biomolecules Article Recognition of elements of protein tertiary structure is crucial for biotechnological and biomedical tasks; this makes the development of optical sensors for certain protein surface elements important. Herein, we demonstrated the ability of iron(II) clathrochelates (1–3) functionalized with mono-, di- and hexa-carboxyalkylsulfide to induce selective circular dichroism (CD) response upon binding to globular proteins. Thus, inherently CD-silent clathrochelates revealed selective inducing of CD spectra when binding to human serum albumin (HSA) (1, 2), beta-lactoglobuline (2) and bovine serum albumin (BSA) (3). Hence, functionalization of iron(II) clathrochelates with the carboxyalkylsulfide group appears to be a promising tool for the design of CD-probes sensitive to certain surface elements of proteins tertiary structure. Additionally, interaction of 1–3 with proteins was also studied by isothermal titration calorimetry, protein fluorescence quenching, electrospray ionization mass spectrometry (ESI-MS) and computer simulations. Formation of both 1:1 and 1:2 assemblies of HSA with 1–3 was evidenced by ESI-MS. A protein fluorescence quenching study suggests that 3 binds with both BSA and HSA via the sites close to Trp residues. Molecular docking calculations indicate that for both BSA and HSA, binding of 3 to Site I and to an “additional site” is more favorable energetically than binding to Site II. MDPI 2020-11-26 /pmc/articles/PMC7759900/ /pubmed/33256144 http://dx.doi.org/10.3390/biom10121602 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Losytskyy, Mykhaylo
Chornenka, Nina
Vakarov, Serhii
Meier-Menches, Samuel M.
Gerner, Christopher
Potocki, Slawomir
Arion, Vladimir B.
Gumienna-Kontecka, Elzbieta
Voloshin, Yan
Kovalska, Vladyslava
Sensing of Proteins by ICD Response of Iron(II) Clathrochelates Functionalized by Carboxyalkylsulfide Groups
title Sensing of Proteins by ICD Response of Iron(II) Clathrochelates Functionalized by Carboxyalkylsulfide Groups
title_full Sensing of Proteins by ICD Response of Iron(II) Clathrochelates Functionalized by Carboxyalkylsulfide Groups
title_fullStr Sensing of Proteins by ICD Response of Iron(II) Clathrochelates Functionalized by Carboxyalkylsulfide Groups
title_full_unstemmed Sensing of Proteins by ICD Response of Iron(II) Clathrochelates Functionalized by Carboxyalkylsulfide Groups
title_short Sensing of Proteins by ICD Response of Iron(II) Clathrochelates Functionalized by Carboxyalkylsulfide Groups
title_sort sensing of proteins by icd response of iron(ii) clathrochelates functionalized by carboxyalkylsulfide groups
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759900/
https://www.ncbi.nlm.nih.gov/pubmed/33256144
http://dx.doi.org/10.3390/biom10121602
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