Cargando…

Shotgun Metagenomic Sequencing Identifies Dysbiosis in Triplet Sibling with Gastrointestinal Symptoms and ASD

The gut microbiome profile of a child with autism spectrum disorder (ASD) and co-occurring gastrointestinal (GI) symptoms was compared to that of her healthy triplet siblings to determine if she exhibited intestinal dysbiosis. Shotgun metagenomic sequencing was performed in individual fecal samples,...

Descripción completa

Detalles Bibliográficos
Autores principales: Hazan, Sabine, Spradling-Reeves, Kimberly D., Papoutsis, Andreas, Walker, Stephen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759978/
https://www.ncbi.nlm.nih.gov/pubmed/33255785
http://dx.doi.org/10.3390/children7120255
_version_ 1783627223841898496
author Hazan, Sabine
Spradling-Reeves, Kimberly D.
Papoutsis, Andreas
Walker, Stephen J.
author_facet Hazan, Sabine
Spradling-Reeves, Kimberly D.
Papoutsis, Andreas
Walker, Stephen J.
author_sort Hazan, Sabine
collection PubMed
description The gut microbiome profile of a child with autism spectrum disorder (ASD) and co-occurring gastrointestinal (GI) symptoms was compared to that of her healthy triplet siblings to determine if she exhibited intestinal dysbiosis. Shotgun metagenomic sequencing was performed in individual fecal samples, and relative microbial abundance and diversity was determined. Microbial diversity was lower in sibling #3, coupled with a higher Bacteroidetes/Firmicutes ratio, a lower relative abundance of Actinobacteria, and an increased relative abundance of Proteobacteria. Our findings are suggestive of gut dysbiosis in a child with ASD and co-occurring GI symptoms, compared to her two healthy triplet siblings.
format Online
Article
Text
id pubmed-7759978
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77599782020-12-26 Shotgun Metagenomic Sequencing Identifies Dysbiosis in Triplet Sibling with Gastrointestinal Symptoms and ASD Hazan, Sabine Spradling-Reeves, Kimberly D. Papoutsis, Andreas Walker, Stephen J. Children (Basel) Brief Report The gut microbiome profile of a child with autism spectrum disorder (ASD) and co-occurring gastrointestinal (GI) symptoms was compared to that of her healthy triplet siblings to determine if she exhibited intestinal dysbiosis. Shotgun metagenomic sequencing was performed in individual fecal samples, and relative microbial abundance and diversity was determined. Microbial diversity was lower in sibling #3, coupled with a higher Bacteroidetes/Firmicutes ratio, a lower relative abundance of Actinobacteria, and an increased relative abundance of Proteobacteria. Our findings are suggestive of gut dysbiosis in a child with ASD and co-occurring GI symptoms, compared to her two healthy triplet siblings. MDPI 2020-11-25 /pmc/articles/PMC7759978/ /pubmed/33255785 http://dx.doi.org/10.3390/children7120255 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Hazan, Sabine
Spradling-Reeves, Kimberly D.
Papoutsis, Andreas
Walker, Stephen J.
Shotgun Metagenomic Sequencing Identifies Dysbiosis in Triplet Sibling with Gastrointestinal Symptoms and ASD
title Shotgun Metagenomic Sequencing Identifies Dysbiosis in Triplet Sibling with Gastrointestinal Symptoms and ASD
title_full Shotgun Metagenomic Sequencing Identifies Dysbiosis in Triplet Sibling with Gastrointestinal Symptoms and ASD
title_fullStr Shotgun Metagenomic Sequencing Identifies Dysbiosis in Triplet Sibling with Gastrointestinal Symptoms and ASD
title_full_unstemmed Shotgun Metagenomic Sequencing Identifies Dysbiosis in Triplet Sibling with Gastrointestinal Symptoms and ASD
title_short Shotgun Metagenomic Sequencing Identifies Dysbiosis in Triplet Sibling with Gastrointestinal Symptoms and ASD
title_sort shotgun metagenomic sequencing identifies dysbiosis in triplet sibling with gastrointestinal symptoms and asd
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759978/
https://www.ncbi.nlm.nih.gov/pubmed/33255785
http://dx.doi.org/10.3390/children7120255
work_keys_str_mv AT hazansabine shotgunmetagenomicsequencingidentifiesdysbiosisintripletsiblingwithgastrointestinalsymptomsandasd
AT spradlingreeveskimberlyd shotgunmetagenomicsequencingidentifiesdysbiosisintripletsiblingwithgastrointestinalsymptomsandasd
AT papoutsisandreas shotgunmetagenomicsequencingidentifiesdysbiosisintripletsiblingwithgastrointestinalsymptomsandasd
AT walkerstephenj shotgunmetagenomicsequencingidentifiesdysbiosisintripletsiblingwithgastrointestinalsymptomsandasd