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Hypoxia as a Driving Force of Pluripotent Stem Cell Reprogramming and Differentiation to Endothelial Cells

Inadequate supply of oxygen (O(2)) is a hallmark of many diseases, in particular those related to the cardiovascular system. On the other hand, tissue hypoxia is an important factor regulating (normal) embryogenesis and differentiation of stem cells at the early stages of embryonic development. In c...

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Autores principales: Podkalicka, Paulina, Stępniewski, Jacek, Mucha, Olga, Kachamakova-Trojanowska, Neli, Dulak, Józef, Łoboda, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759989/
https://www.ncbi.nlm.nih.gov/pubmed/33260307
http://dx.doi.org/10.3390/biom10121614
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author Podkalicka, Paulina
Stępniewski, Jacek
Mucha, Olga
Kachamakova-Trojanowska, Neli
Dulak, Józef
Łoboda, Agnieszka
author_facet Podkalicka, Paulina
Stępniewski, Jacek
Mucha, Olga
Kachamakova-Trojanowska, Neli
Dulak, Józef
Łoboda, Agnieszka
author_sort Podkalicka, Paulina
collection PubMed
description Inadequate supply of oxygen (O(2)) is a hallmark of many diseases, in particular those related to the cardiovascular system. On the other hand, tissue hypoxia is an important factor regulating (normal) embryogenesis and differentiation of stem cells at the early stages of embryonic development. In culture, hypoxic conditions may facilitate the derivation of embryonic stem cells (ESCs) and the generation of induced pluripotent stem cells (iPSCs), which may serve as a valuable tool for disease modeling. Endothelial cells (ECs), multifunctional components of vascular structures, may be obtained from iPSCs and subsequently used in various (hypoxia-related) disease models to investigate vascular dysfunctions. Although iPSC-ECs demonstrated functionality in vitro and in vivo, ongoing studies are conducted to increase the efficiency of differentiation and to establish the most productive protocols for the application of patient-derived cells in clinics. In this review, we highlight recent discoveries on the role of hypoxia in the derivation of ESCs and the generation of iPSCs. We also summarize the existing protocols of hypoxia-driven differentiation of iPSCs toward ECs and discuss their possible applications in disease modeling and treatment of hypoxia-related disorders.
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spelling pubmed-77599892020-12-26 Hypoxia as a Driving Force of Pluripotent Stem Cell Reprogramming and Differentiation to Endothelial Cells Podkalicka, Paulina Stępniewski, Jacek Mucha, Olga Kachamakova-Trojanowska, Neli Dulak, Józef Łoboda, Agnieszka Biomolecules Review Inadequate supply of oxygen (O(2)) is a hallmark of many diseases, in particular those related to the cardiovascular system. On the other hand, tissue hypoxia is an important factor regulating (normal) embryogenesis and differentiation of stem cells at the early stages of embryonic development. In culture, hypoxic conditions may facilitate the derivation of embryonic stem cells (ESCs) and the generation of induced pluripotent stem cells (iPSCs), which may serve as a valuable tool for disease modeling. Endothelial cells (ECs), multifunctional components of vascular structures, may be obtained from iPSCs and subsequently used in various (hypoxia-related) disease models to investigate vascular dysfunctions. Although iPSC-ECs demonstrated functionality in vitro and in vivo, ongoing studies are conducted to increase the efficiency of differentiation and to establish the most productive protocols for the application of patient-derived cells in clinics. In this review, we highlight recent discoveries on the role of hypoxia in the derivation of ESCs and the generation of iPSCs. We also summarize the existing protocols of hypoxia-driven differentiation of iPSCs toward ECs and discuss their possible applications in disease modeling and treatment of hypoxia-related disorders. MDPI 2020-11-29 /pmc/articles/PMC7759989/ /pubmed/33260307 http://dx.doi.org/10.3390/biom10121614 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Podkalicka, Paulina
Stępniewski, Jacek
Mucha, Olga
Kachamakova-Trojanowska, Neli
Dulak, Józef
Łoboda, Agnieszka
Hypoxia as a Driving Force of Pluripotent Stem Cell Reprogramming and Differentiation to Endothelial Cells
title Hypoxia as a Driving Force of Pluripotent Stem Cell Reprogramming and Differentiation to Endothelial Cells
title_full Hypoxia as a Driving Force of Pluripotent Stem Cell Reprogramming and Differentiation to Endothelial Cells
title_fullStr Hypoxia as a Driving Force of Pluripotent Stem Cell Reprogramming and Differentiation to Endothelial Cells
title_full_unstemmed Hypoxia as a Driving Force of Pluripotent Stem Cell Reprogramming and Differentiation to Endothelial Cells
title_short Hypoxia as a Driving Force of Pluripotent Stem Cell Reprogramming and Differentiation to Endothelial Cells
title_sort hypoxia as a driving force of pluripotent stem cell reprogramming and differentiation to endothelial cells
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759989/
https://www.ncbi.nlm.nih.gov/pubmed/33260307
http://dx.doi.org/10.3390/biom10121614
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