Cargando…
Vasorelaxant Effect of Boesenbergia rotunda and Its Active Ingredients on an Isolated Coronary Artery
Cardiovascular diseases are a major cause of death in developed countries. The regulation of vascular tone is a major approach to prevent and ameliorate vascular diseases. As part of our ongoing screening for cardioprotective natural compounds, we investigated the vasorelaxant effect of rhizomes fro...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760037/ https://www.ncbi.nlm.nih.gov/pubmed/33271853 http://dx.doi.org/10.3390/plants9121688 |
_version_ | 1783627237727141888 |
---|---|
author | Adhikari, Deepak Gong, Dal-Seong Oh, Se Hee Sung, Eun Hee Lee, Seung On Kim, Dong-Wook Oak, Min-Ho Kim, Hyun Jung |
author_facet | Adhikari, Deepak Gong, Dal-Seong Oh, Se Hee Sung, Eun Hee Lee, Seung On Kim, Dong-Wook Oak, Min-Ho Kim, Hyun Jung |
author_sort | Adhikari, Deepak |
collection | PubMed |
description | Cardiovascular diseases are a major cause of death in developed countries. The regulation of vascular tone is a major approach to prevent and ameliorate vascular diseases. As part of our ongoing screening for cardioprotective natural compounds, we investigated the vasorelaxant effect of rhizomes from Boesenbergia rotunda (L.) Mansf. [Boesenbergia pandurata (Roxb.) Schltr.] used as a spice and herbal medicine in Asian countries. The methanol extract of B. rotunda rhizomes (BRE) exhibited significant vasorelaxation effects ex vivo at EC(50) values of 13.4 ± 6.1 μg/mL and 40.9 ± 7.9 μg/mL, respectively, with and without endothelium in the porcine coronary artery ring. The intrinsic mechanism was evaluated by treating with specific inhibitors or activators that typically affect vascular reactivity. The results suggested that BRE induced relaxation in the coronary artery rings via an endothelium-dependent pathway involving NO-cGMP, and also via an endothelium-independent pathway involving the blockade of Ca(2+) channels. Vasorelaxant principles in BRE were identified by subsequent chromatographic methods, which revealed that flavonoids regulate vasorelaxant activity in BRE. One of the flavonoids was a Diels-Alder type adduct, 4-hydroxypanduratin A, which showed the most potent vasorelaxant effect on porcine coronary artery with an EC(50) of 17.8 ± 2.5 μM. Our results suggest that rhizomes of B. rotunda might be of interest as herbal medicine against cardiovascular diseases. |
format | Online Article Text |
id | pubmed-7760037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77600372020-12-26 Vasorelaxant Effect of Boesenbergia rotunda and Its Active Ingredients on an Isolated Coronary Artery Adhikari, Deepak Gong, Dal-Seong Oh, Se Hee Sung, Eun Hee Lee, Seung On Kim, Dong-Wook Oak, Min-Ho Kim, Hyun Jung Plants (Basel) Article Cardiovascular diseases are a major cause of death in developed countries. The regulation of vascular tone is a major approach to prevent and ameliorate vascular diseases. As part of our ongoing screening for cardioprotective natural compounds, we investigated the vasorelaxant effect of rhizomes from Boesenbergia rotunda (L.) Mansf. [Boesenbergia pandurata (Roxb.) Schltr.] used as a spice and herbal medicine in Asian countries. The methanol extract of B. rotunda rhizomes (BRE) exhibited significant vasorelaxation effects ex vivo at EC(50) values of 13.4 ± 6.1 μg/mL and 40.9 ± 7.9 μg/mL, respectively, with and without endothelium in the porcine coronary artery ring. The intrinsic mechanism was evaluated by treating with specific inhibitors or activators that typically affect vascular reactivity. The results suggested that BRE induced relaxation in the coronary artery rings via an endothelium-dependent pathway involving NO-cGMP, and also via an endothelium-independent pathway involving the blockade of Ca(2+) channels. Vasorelaxant principles in BRE were identified by subsequent chromatographic methods, which revealed that flavonoids regulate vasorelaxant activity in BRE. One of the flavonoids was a Diels-Alder type adduct, 4-hydroxypanduratin A, which showed the most potent vasorelaxant effect on porcine coronary artery with an EC(50) of 17.8 ± 2.5 μM. Our results suggest that rhizomes of B. rotunda might be of interest as herbal medicine against cardiovascular diseases. MDPI 2020-12-01 /pmc/articles/PMC7760037/ /pubmed/33271853 http://dx.doi.org/10.3390/plants9121688 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Adhikari, Deepak Gong, Dal-Seong Oh, Se Hee Sung, Eun Hee Lee, Seung On Kim, Dong-Wook Oak, Min-Ho Kim, Hyun Jung Vasorelaxant Effect of Boesenbergia rotunda and Its Active Ingredients on an Isolated Coronary Artery |
title | Vasorelaxant Effect of Boesenbergia rotunda and Its Active Ingredients on an Isolated Coronary Artery |
title_full | Vasorelaxant Effect of Boesenbergia rotunda and Its Active Ingredients on an Isolated Coronary Artery |
title_fullStr | Vasorelaxant Effect of Boesenbergia rotunda and Its Active Ingredients on an Isolated Coronary Artery |
title_full_unstemmed | Vasorelaxant Effect of Boesenbergia rotunda and Its Active Ingredients on an Isolated Coronary Artery |
title_short | Vasorelaxant Effect of Boesenbergia rotunda and Its Active Ingredients on an Isolated Coronary Artery |
title_sort | vasorelaxant effect of boesenbergia rotunda and its active ingredients on an isolated coronary artery |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760037/ https://www.ncbi.nlm.nih.gov/pubmed/33271853 http://dx.doi.org/10.3390/plants9121688 |
work_keys_str_mv | AT adhikarideepak vasorelaxanteffectofboesenbergiarotundaanditsactiveingredientsonanisolatedcoronaryartery AT gongdalseong vasorelaxanteffectofboesenbergiarotundaanditsactiveingredientsonanisolatedcoronaryartery AT ohsehee vasorelaxanteffectofboesenbergiarotundaanditsactiveingredientsonanisolatedcoronaryartery AT sungeunhee vasorelaxanteffectofboesenbergiarotundaanditsactiveingredientsonanisolatedcoronaryartery AT leeseungon vasorelaxanteffectofboesenbergiarotundaanditsactiveingredientsonanisolatedcoronaryartery AT kimdongwook vasorelaxanteffectofboesenbergiarotundaanditsactiveingredientsonanisolatedcoronaryartery AT oakminho vasorelaxanteffectofboesenbergiarotundaanditsactiveingredientsonanisolatedcoronaryartery AT kimhyunjung vasorelaxanteffectofboesenbergiarotundaanditsactiveingredientsonanisolatedcoronaryartery |