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Well Defined Poly(Methyl Methacrylate)-Fe(3)O(4)/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells

The objective of this work is to develop and characterize polymeric nanoparticles with core–shell morphology through miniemulsion polymerization combined with seeded emulsion polymerization, aiming at the application in the treatment of vascular tumors via intravascular embolization. The synthesis o...

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Detalles Bibliográficos
Autores principales: Resende, Graciane, Dutra, Gabriel V. S., Neta, Maria S. B., Araújo, Olacir A., Chaves, Sacha B., Machado, Fabricio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760038/
https://www.ncbi.nlm.nih.gov/pubmed/33266092
http://dx.doi.org/10.3390/polym12122868
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author Resende, Graciane
Dutra, Gabriel V. S.
Neta, Maria S. B.
Araújo, Olacir A.
Chaves, Sacha B.
Machado, Fabricio
author_facet Resende, Graciane
Dutra, Gabriel V. S.
Neta, Maria S. B.
Araújo, Olacir A.
Chaves, Sacha B.
Machado, Fabricio
author_sort Resende, Graciane
collection PubMed
description The objective of this work is to develop and characterize polymeric nanoparticles with core–shell morphology through miniemulsion polymerization combined with seeded emulsion polymerization, aiming at the application in the treatment of vascular tumors via intravascular embolization. The synthesis of the core–shell nanocomposites was divided into two main steps: (i) Formation of the core structure, consisting of poly(methyl methacrylate)/magnetic oxide coated with oleic acid (OM-OA) via miniemulsion and (ii) shell structure produced through seeded emulsion polymerization of vinyl pivalate. Nanocomposites containing about 8 wt.% of OM-OA showed high colloidal stability, mean diameter of 216.8 nm, spherical morphology, saturation magnetization (M(s)) of 4.65 emu·g(−1) (57.41 emu·g(−1) of Fe(3)O(4)), preserved superparamagnetic behavior and glass transition temperature (T(g)) of 111.8 °C. TEM micrographs confirmed the obtaining of uniformly dispersed magnetic nanoparticles in the PMMA and that the core–shell structure was obtained by seeded emulsion with M(s) of 1.35 emu·g(−1) (56.25 emu·g(−1) of Fe(3)O(4)) and T(g) of 114.7 °C. In vitro cytotoxicity assays against murine tumor of melanoma (B16F10) and human Keratinocytes (HaCaT) cell lines were carried out showing that the core–shell magnetic polymeric materials (a core, consisting of poly(methyl methacrylate)/Fe(3)O(4) and, a shell, formed by poly(vinyl pivalate)) presented high cell viabilities for both murine melanoma tumor cell lines, B16F10, and human keratinocyte cells, HaCaT.
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spelling pubmed-77600382020-12-26 Well Defined Poly(Methyl Methacrylate)-Fe(3)O(4)/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells Resende, Graciane Dutra, Gabriel V. S. Neta, Maria S. B. Araújo, Olacir A. Chaves, Sacha B. Machado, Fabricio Polymers (Basel) Article The objective of this work is to develop and characterize polymeric nanoparticles with core–shell morphology through miniemulsion polymerization combined with seeded emulsion polymerization, aiming at the application in the treatment of vascular tumors via intravascular embolization. The synthesis of the core–shell nanocomposites was divided into two main steps: (i) Formation of the core structure, consisting of poly(methyl methacrylate)/magnetic oxide coated with oleic acid (OM-OA) via miniemulsion and (ii) shell structure produced through seeded emulsion polymerization of vinyl pivalate. Nanocomposites containing about 8 wt.% of OM-OA showed high colloidal stability, mean diameter of 216.8 nm, spherical morphology, saturation magnetization (M(s)) of 4.65 emu·g(−1) (57.41 emu·g(−1) of Fe(3)O(4)), preserved superparamagnetic behavior and glass transition temperature (T(g)) of 111.8 °C. TEM micrographs confirmed the obtaining of uniformly dispersed magnetic nanoparticles in the PMMA and that the core–shell structure was obtained by seeded emulsion with M(s) of 1.35 emu·g(−1) (56.25 emu·g(−1) of Fe(3)O(4)) and T(g) of 114.7 °C. In vitro cytotoxicity assays against murine tumor of melanoma (B16F10) and human Keratinocytes (HaCaT) cell lines were carried out showing that the core–shell magnetic polymeric materials (a core, consisting of poly(methyl methacrylate)/Fe(3)O(4) and, a shell, formed by poly(vinyl pivalate)) presented high cell viabilities for both murine melanoma tumor cell lines, B16F10, and human keratinocyte cells, HaCaT. MDPI 2020-11-30 /pmc/articles/PMC7760038/ /pubmed/33266092 http://dx.doi.org/10.3390/polym12122868 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Resende, Graciane
Dutra, Gabriel V. S.
Neta, Maria S. B.
Araújo, Olacir A.
Chaves, Sacha B.
Machado, Fabricio
Well Defined Poly(Methyl Methacrylate)-Fe(3)O(4)/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells
title Well Defined Poly(Methyl Methacrylate)-Fe(3)O(4)/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells
title_full Well Defined Poly(Methyl Methacrylate)-Fe(3)O(4)/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells
title_fullStr Well Defined Poly(Methyl Methacrylate)-Fe(3)O(4)/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells
title_full_unstemmed Well Defined Poly(Methyl Methacrylate)-Fe(3)O(4)/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells
title_short Well Defined Poly(Methyl Methacrylate)-Fe(3)O(4)/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells
title_sort well defined poly(methyl methacrylate)-fe(3)o(4)/poly(vinyl pivalate) core–shell superparamagnetic nanoparticles: design and evaluation of in vitro cytotoxicity activity against cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760038/
https://www.ncbi.nlm.nih.gov/pubmed/33266092
http://dx.doi.org/10.3390/polym12122868
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