In Vitro Antibacterial Activity of Manuka (Leptospermum scoparium J.R. et G. Forst) and winter Savory (Satureja montana L.) Essential Oils and Their Blends against Pathogenic E. coli Isolates from Pigs

SIMPLE SUMMARY: Neonatal diarrhoea (ND), post-weaning diarrhoea (PWD) and oedema disease (OD) are particularly important in terms of economic losses in pig farming worldwide. Aetiological agents of these diseases belong to Escherichia coli, in particular to a few serogroups of enterotoxigenic Escherichia coli (ETEC) and enteropathogenic Escherichia coli (EPEC). The necessity for a reduction in antibiotic use, related to the growing antibiotic resistance phenomenon, encourages research in the study of alternative antibacterial substances as antibacterial tools. Essential oils could represent a valid solution. ABSTRACT: Neonatal diarrhoea (ND), post-weaning diarrhoea (PWD) and oedema disease (OD) are among the most important diseases affecting pig farming due to economic losses. Among the main aetiological agents, strains of Escherichia coli are identified as the major responsible pathogens involved. Several strategies have been put in place to prevent these infections and, today, research is increasingly studying alternative methods to antibiotics to reduce the antibiotic resistance phenomenon. Essential oils (EOs) are among the alternative tools that are being investigated. In this study, the in vitro effectiveness of winter savory and manuka essential oils and their mixtures in different proportions against strains of E. coli isolated from episodes of disease in pigs was evaluated. The EOs alone demonstrated slight antibacterial effectiveness, whereas the blends, by virtue of their synergistic action, showed remarkable activity, especially the 70%–30% winter savory–manuka blend, showing itself as a potential tool for prevention and therapy.