Cargando…
Synthetic Extracellular Matrices as a Toolbox to Tune Stem Cell Secretome
[Image: see text] The application of stem cell-derived secretome in regenerative therapies offers the key advantage that instead of the stem cells, only their effective paracrine compounds are in vivo delivered. Ideally, the secretome can be steered by the culture conditions of the stem cells. So fa...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2020
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760093/ https://www.ncbi.nlm.nih.gov/pubmed/33305561 http://dx.doi.org/10.1021/acsami.0c16208 |
_version_ | 1783627250970656768 |
---|---|
author | Liu, Kaizheng Veenendaal, Tomas Wiendels, Maury Ruiz-Zapata, Alejandra M. van Laar, Justin Kyranas, Rafail Enting, Hilde van Cranenbroek, Bram Koenen, Hans J. P. M. Mihaila, Silvia M. Oosterwijk, Egbert Kouwer, Paul H. J. |
author_facet | Liu, Kaizheng Veenendaal, Tomas Wiendels, Maury Ruiz-Zapata, Alejandra M. van Laar, Justin Kyranas, Rafail Enting, Hilde van Cranenbroek, Bram Koenen, Hans J. P. M. Mihaila, Silvia M. Oosterwijk, Egbert Kouwer, Paul H. J. |
author_sort | Liu, Kaizheng |
collection | PubMed |
description | [Image: see text] The application of stem cell-derived secretome in regenerative therapies offers the key advantage that instead of the stem cells, only their effective paracrine compounds are in vivo delivered. Ideally, the secretome can be steered by the culture conditions of the stem cells. So far, most studies use stem cells cultured on stiff plastic substrates, not representative of their native 3D environment. In this study, cells are cultured inside synthetic polyisocyanide (PIC)-based hydrogels, which are minimal, tailorable, and highly reproducible biomimetic matrices. Secretome analysis of human adipose-derived stem cells (multiplex, ELISA) displays that matrix manipulation is a powerful tool to direct the secretome composition. As an example, cells in nonadherent PIC gels secrete increased levels of IL-10 and the conditioned media from 3D culture accelerate wound closure. In all, our PIC-based approach opens the door to dedicated matrix design to engineer the secretome for custom applications. |
format | Online Article Text |
id | pubmed-7760093 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-77600932020-12-28 Synthetic Extracellular Matrices as a Toolbox to Tune Stem Cell Secretome Liu, Kaizheng Veenendaal, Tomas Wiendels, Maury Ruiz-Zapata, Alejandra M. van Laar, Justin Kyranas, Rafail Enting, Hilde van Cranenbroek, Bram Koenen, Hans J. P. M. Mihaila, Silvia M. Oosterwijk, Egbert Kouwer, Paul H. J. ACS Appl Mater Interfaces [Image: see text] The application of stem cell-derived secretome in regenerative therapies offers the key advantage that instead of the stem cells, only their effective paracrine compounds are in vivo delivered. Ideally, the secretome can be steered by the culture conditions of the stem cells. So far, most studies use stem cells cultured on stiff plastic substrates, not representative of their native 3D environment. In this study, cells are cultured inside synthetic polyisocyanide (PIC)-based hydrogels, which are minimal, tailorable, and highly reproducible biomimetic matrices. Secretome analysis of human adipose-derived stem cells (multiplex, ELISA) displays that matrix manipulation is a powerful tool to direct the secretome composition. As an example, cells in nonadherent PIC gels secrete increased levels of IL-10 and the conditioned media from 3D culture accelerate wound closure. In all, our PIC-based approach opens the door to dedicated matrix design to engineer the secretome for custom applications. American Chemical Society 2020-12-11 2020-12-23 /pmc/articles/PMC7760093/ /pubmed/33305561 http://dx.doi.org/10.1021/acsami.0c16208 Text en © 2020 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
spellingShingle | Liu, Kaizheng Veenendaal, Tomas Wiendels, Maury Ruiz-Zapata, Alejandra M. van Laar, Justin Kyranas, Rafail Enting, Hilde van Cranenbroek, Bram Koenen, Hans J. P. M. Mihaila, Silvia M. Oosterwijk, Egbert Kouwer, Paul H. J. Synthetic Extracellular Matrices as a Toolbox to Tune Stem Cell Secretome |
title | Synthetic
Extracellular Matrices as a Toolbox to Tune
Stem Cell Secretome |
title_full | Synthetic
Extracellular Matrices as a Toolbox to Tune
Stem Cell Secretome |
title_fullStr | Synthetic
Extracellular Matrices as a Toolbox to Tune
Stem Cell Secretome |
title_full_unstemmed | Synthetic
Extracellular Matrices as a Toolbox to Tune
Stem Cell Secretome |
title_short | Synthetic
Extracellular Matrices as a Toolbox to Tune
Stem Cell Secretome |
title_sort | synthetic
extracellular matrices as a toolbox to tune
stem cell secretome |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760093/ https://www.ncbi.nlm.nih.gov/pubmed/33305561 http://dx.doi.org/10.1021/acsami.0c16208 |
work_keys_str_mv | AT liukaizheng syntheticextracellularmatricesasatoolboxtotunestemcellsecretome AT veenendaaltomas syntheticextracellularmatricesasatoolboxtotunestemcellsecretome AT wiendelsmaury syntheticextracellularmatricesasatoolboxtotunestemcellsecretome AT ruizzapataalejandram syntheticextracellularmatricesasatoolboxtotunestemcellsecretome AT vanlaarjustin syntheticextracellularmatricesasatoolboxtotunestemcellsecretome AT kyranasrafail syntheticextracellularmatricesasatoolboxtotunestemcellsecretome AT entinghilde syntheticextracellularmatricesasatoolboxtotunestemcellsecretome AT vancranenbroekbram syntheticextracellularmatricesasatoolboxtotunestemcellsecretome AT koenenhansjpm syntheticextracellularmatricesasatoolboxtotunestemcellsecretome AT mihailasilviam syntheticextracellularmatricesasatoolboxtotunestemcellsecretome AT oosterwijkegbert syntheticextracellularmatricesasatoolboxtotunestemcellsecretome AT kouwerpaulhj syntheticextracellularmatricesasatoolboxtotunestemcellsecretome |