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Metabolic Profiling of Hybrids Generated from Pummelo and Citrus latipes in Relation to Their Attraction to Diaphorina citri, the Vector of Huanglongbing

The citrus industry at present is severely affected by huanglongbing disease (HLB). HLB is caused by the supposed bacterial pathogen “Candidatus Liberibacter asiaticus” and is transmitted by the insect vector, the Asian citrus psyllid, Diaphorina citri Kuwayama. Developing new citrus hybrids to impr...

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Autores principales: Killiny, Nabil, Jones, Shelley E., Hijaz, Faraj, Kishk, Abdelaziz, Santos-Ortega, Yulica, Nehela, Yasser, Omar, Ahmad A., Yu, Qibin, Gmitter, Fred G., Grosser, Jude W., Dutt, Manjul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760127/
https://www.ncbi.nlm.nih.gov/pubmed/33255226
http://dx.doi.org/10.3390/metabo10120477
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author Killiny, Nabil
Jones, Shelley E.
Hijaz, Faraj
Kishk, Abdelaziz
Santos-Ortega, Yulica
Nehela, Yasser
Omar, Ahmad A.
Yu, Qibin
Gmitter, Fred G.
Grosser, Jude W.
Dutt, Manjul
author_facet Killiny, Nabil
Jones, Shelley E.
Hijaz, Faraj
Kishk, Abdelaziz
Santos-Ortega, Yulica
Nehela, Yasser
Omar, Ahmad A.
Yu, Qibin
Gmitter, Fred G.
Grosser, Jude W.
Dutt, Manjul
author_sort Killiny, Nabil
collection PubMed
description The citrus industry at present is severely affected by huanglongbing disease (HLB). HLB is caused by the supposed bacterial pathogen “Candidatus Liberibacter asiaticus” and is transmitted by the insect vector, the Asian citrus psyllid, Diaphorina citri Kuwayama. Developing new citrus hybrids to improve HLB management is much needed. In this study, we investigated the metabolomic profiles of three new hybrids produced from the cross of C2-5-12 Pummelo (Citrus maxima (L.) Osbeck) × pollen from Citrus latipes. The hybrids were selected based on leaf morphology and seedling vigor. The selected hybrids exhibited compact and upright tree architecture as seen in C. latipes. Hybrids were verified by simple sequence repeat markers, and were subjected to metabolomic analysis using gas chromatography-mass spectrometry. The volatile organic compounds (VOCs) and polar metabolites profiling also showed that the new hybrids were different from their parents. Interestingly, the levels of stored VOCs in hybrid II were higher than those observed in its parents and other hybrids. The level of most VOCs released by hybrid II was also higher than that released from its parents. Additionally, the preference assay showed that hybrid II was more attractive to D. citri than its parents and other hybrids. The leaf morphology, compact and upright architecture of hybrid II, and its attraction to D. citri suggest that it could be used as a windbreak and trap tree for D. citri (double duty), once its tolerance to HLB disease is confirmed. Our results showed that metabolomic analysis could be successfully used to understand the biochemical mechanisms controlling the interaction of D. citri with its host plants.
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spelling pubmed-77601272020-12-26 Metabolic Profiling of Hybrids Generated from Pummelo and Citrus latipes in Relation to Their Attraction to Diaphorina citri, the Vector of Huanglongbing Killiny, Nabil Jones, Shelley E. Hijaz, Faraj Kishk, Abdelaziz Santos-Ortega, Yulica Nehela, Yasser Omar, Ahmad A. Yu, Qibin Gmitter, Fred G. Grosser, Jude W. Dutt, Manjul Metabolites Article The citrus industry at present is severely affected by huanglongbing disease (HLB). HLB is caused by the supposed bacterial pathogen “Candidatus Liberibacter asiaticus” and is transmitted by the insect vector, the Asian citrus psyllid, Diaphorina citri Kuwayama. Developing new citrus hybrids to improve HLB management is much needed. In this study, we investigated the metabolomic profiles of three new hybrids produced from the cross of C2-5-12 Pummelo (Citrus maxima (L.) Osbeck) × pollen from Citrus latipes. The hybrids were selected based on leaf morphology and seedling vigor. The selected hybrids exhibited compact and upright tree architecture as seen in C. latipes. Hybrids were verified by simple sequence repeat markers, and were subjected to metabolomic analysis using gas chromatography-mass spectrometry. The volatile organic compounds (VOCs) and polar metabolites profiling also showed that the new hybrids were different from their parents. Interestingly, the levels of stored VOCs in hybrid II were higher than those observed in its parents and other hybrids. The level of most VOCs released by hybrid II was also higher than that released from its parents. Additionally, the preference assay showed that hybrid II was more attractive to D. citri than its parents and other hybrids. The leaf morphology, compact and upright architecture of hybrid II, and its attraction to D. citri suggest that it could be used as a windbreak and trap tree for D. citri (double duty), once its tolerance to HLB disease is confirmed. Our results showed that metabolomic analysis could be successfully used to understand the biochemical mechanisms controlling the interaction of D. citri with its host plants. MDPI 2020-11-24 /pmc/articles/PMC7760127/ /pubmed/33255226 http://dx.doi.org/10.3390/metabo10120477 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Killiny, Nabil
Jones, Shelley E.
Hijaz, Faraj
Kishk, Abdelaziz
Santos-Ortega, Yulica
Nehela, Yasser
Omar, Ahmad A.
Yu, Qibin
Gmitter, Fred G.
Grosser, Jude W.
Dutt, Manjul
Metabolic Profiling of Hybrids Generated from Pummelo and Citrus latipes in Relation to Their Attraction to Diaphorina citri, the Vector of Huanglongbing
title Metabolic Profiling of Hybrids Generated from Pummelo and Citrus latipes in Relation to Their Attraction to Diaphorina citri, the Vector of Huanglongbing
title_full Metabolic Profiling of Hybrids Generated from Pummelo and Citrus latipes in Relation to Their Attraction to Diaphorina citri, the Vector of Huanglongbing
title_fullStr Metabolic Profiling of Hybrids Generated from Pummelo and Citrus latipes in Relation to Their Attraction to Diaphorina citri, the Vector of Huanglongbing
title_full_unstemmed Metabolic Profiling of Hybrids Generated from Pummelo and Citrus latipes in Relation to Their Attraction to Diaphorina citri, the Vector of Huanglongbing
title_short Metabolic Profiling of Hybrids Generated from Pummelo and Citrus latipes in Relation to Their Attraction to Diaphorina citri, the Vector of Huanglongbing
title_sort metabolic profiling of hybrids generated from pummelo and citrus latipes in relation to their attraction to diaphorina citri, the vector of huanglongbing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760127/
https://www.ncbi.nlm.nih.gov/pubmed/33255226
http://dx.doi.org/10.3390/metabo10120477
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