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Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus)
SIMPLE SUMMARY: The study of chicken embryonic stem cells (ESCs) and primordial germ cells (PGCs) showed the potential application of developmental biology and translational medicine. However, the difference between amphoteric ESCs and PGCs is still elusive, limiting the accuracy of correlative rese...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760303/ https://www.ncbi.nlm.nih.gov/pubmed/33261034 http://dx.doi.org/10.3390/ani10122228 |
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author | Jin, Kai Zhou, Jing Zuo, Qisheng Song, Jiuzhou Zhang, Yani Chang, Guobing Chen, Guohong Li, Bichun |
author_facet | Jin, Kai Zhou, Jing Zuo, Qisheng Song, Jiuzhou Zhang, Yani Chang, Guobing Chen, Guohong Li, Bichun |
author_sort | Jin, Kai |
collection | PubMed |
description | SIMPLE SUMMARY: The study of chicken embryonic stem cells (ESCs) and primordial germ cells (PGCs) showed the potential application of developmental biology and translational medicine. However, the difference between amphoteric ESCs and PGCs is still elusive, limiting the accuracy of correlative research. In this paper, chicken amphoteric ESCs and PGCs were isolated, separated, and sequenced to explore their dynamic transcriptomes. Our results provide a knowledge base of transcriptomes in chicken amphoteric ESCs and PGCs, which will help other researchers interested in studying relative biological processes. ABSTRACT: Chicken (Gallus gallus) pluripotent embryonic stem cells (ESCs) and primordial germ cells (PGCs) can be broadly applied in the research of developmental and embryonic biology, but the difference between amphoteric ESCs and PGCs is still elusive. This study determined the sex of collected samples by identifying specific sex markers via polymerase chain reaction (PCR) and fluorescence activated cell sorter (FACS). RNA-seq was utilized to investigate the transcriptomic profile of amphoteric ESCs and PGCs in chicken. The results showed no significant differentially expressed genes (DEGs) in amphoteric ESCs and 227 DEGs exhibited in amphoteric PGCs. Moreover, those 227 DEGs were mainly enriched in 17 gene ontology (GO) terms and 27 pathways according to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Furthermore, qRT-PCR was performed to verify RNA-seq results, and the results demonstrated that Notch1 was highly expressed in male PGCs. In summary, our results provided a knowledge base of chicken amphoteric ESCs and PGCs, which is helpful for future research in relevant biological processes. |
format | Online Article Text |
id | pubmed-7760303 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77603032020-12-26 Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus) Jin, Kai Zhou, Jing Zuo, Qisheng Song, Jiuzhou Zhang, Yani Chang, Guobing Chen, Guohong Li, Bichun Animals (Basel) Article SIMPLE SUMMARY: The study of chicken embryonic stem cells (ESCs) and primordial germ cells (PGCs) showed the potential application of developmental biology and translational medicine. However, the difference between amphoteric ESCs and PGCs is still elusive, limiting the accuracy of correlative research. In this paper, chicken amphoteric ESCs and PGCs were isolated, separated, and sequenced to explore their dynamic transcriptomes. Our results provide a knowledge base of transcriptomes in chicken amphoteric ESCs and PGCs, which will help other researchers interested in studying relative biological processes. ABSTRACT: Chicken (Gallus gallus) pluripotent embryonic stem cells (ESCs) and primordial germ cells (PGCs) can be broadly applied in the research of developmental and embryonic biology, but the difference between amphoteric ESCs and PGCs is still elusive. This study determined the sex of collected samples by identifying specific sex markers via polymerase chain reaction (PCR) and fluorescence activated cell sorter (FACS). RNA-seq was utilized to investigate the transcriptomic profile of amphoteric ESCs and PGCs in chicken. The results showed no significant differentially expressed genes (DEGs) in amphoteric ESCs and 227 DEGs exhibited in amphoteric PGCs. Moreover, those 227 DEGs were mainly enriched in 17 gene ontology (GO) terms and 27 pathways according to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Furthermore, qRT-PCR was performed to verify RNA-seq results, and the results demonstrated that Notch1 was highly expressed in male PGCs. In summary, our results provided a knowledge base of chicken amphoteric ESCs and PGCs, which is helpful for future research in relevant biological processes. MDPI 2020-11-27 /pmc/articles/PMC7760303/ /pubmed/33261034 http://dx.doi.org/10.3390/ani10122228 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jin, Kai Zhou, Jing Zuo, Qisheng Song, Jiuzhou Zhang, Yani Chang, Guobing Chen, Guohong Li, Bichun Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus) |
title | Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus) |
title_full | Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus) |
title_fullStr | Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus) |
title_full_unstemmed | Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus) |
title_short | Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus) |
title_sort | transcriptome sequencing and comparative analysis of amphoteric escs and pgcs in chicken (gallus gallus) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760303/ https://www.ncbi.nlm.nih.gov/pubmed/33261034 http://dx.doi.org/10.3390/ani10122228 |
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