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Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71

Subunit vaccines consist of non-genetic material, such as peptides or proteins. They are considered safe because they have fewer side effects; however, they have low immunogenicity when used alone. We aimed to enhance the immune response of peptide-based vaccines by using self-assembled multimeric p...

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Autores principales: Kim, Yu-Gyeong, Lee, Yunsu, Kim, Joo Hee, Chang, Sun-Young, Jung, Jong-Wha, Chung, Woo-Jae, Jin, Hyo-Eon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760352/
https://www.ncbi.nlm.nih.gov/pubmed/33255791
http://dx.doi.org/10.3390/nano10122342
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author Kim, Yu-Gyeong
Lee, Yunsu
Kim, Joo Hee
Chang, Sun-Young
Jung, Jong-Wha
Chung, Woo-Jae
Jin, Hyo-Eon
author_facet Kim, Yu-Gyeong
Lee, Yunsu
Kim, Joo Hee
Chang, Sun-Young
Jung, Jong-Wha
Chung, Woo-Jae
Jin, Hyo-Eon
author_sort Kim, Yu-Gyeong
collection PubMed
description Subunit vaccines consist of non-genetic material, such as peptides or proteins. They are considered safe because they have fewer side effects; however, they have low immunogenicity when used alone. We aimed to enhance the immune response of peptide-based vaccines by using self-assembled multimeric peptide amphiphiles (PAs). We designed two epitope PAs by conjugating epitope peptides from Enterovirus 71 (EV71) virus particle (VP) 1 and VP3 capsid proteins with different fatty acid chain lengths (VP1PA and VP3PA). These PAs self-assembled into supramolecular structures at a physiological pH, and the resulting structures were characterized using atomic force microscopy. Multi-epitope PAs (m-PAs) consisted of a 1:1 mixture of VP1PA and VP3PA solutions. To evaluate immunogenicity, m-PA constructs were injected with adjuvant subcutaneously into female Balb/c mice. Levels of antigen-specific immunoglobulin G (IgG) and IgG1 in m-PA-injected mice serum samples were analyzed using ELISA and Western blotting. Additionally, cytokine production stimulated by each antigen was measured in splenocytes cultured from immunized mice groups. We found that m-PA showed improved humoral and cellular immune responses compared to the control and peptide groups. The sera from m-PA immunized mice group could neutralize EV71 infection and protect host cells. Thus, self-assembled m-PAs can promote a protective immune response and can be developed as a potential platform technology to produce peptide vaccines against infectious viral diseases.
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spelling pubmed-77603522020-12-26 Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71 Kim, Yu-Gyeong Lee, Yunsu Kim, Joo Hee Chang, Sun-Young Jung, Jong-Wha Chung, Woo-Jae Jin, Hyo-Eon Nanomaterials (Basel) Article Subunit vaccines consist of non-genetic material, such as peptides or proteins. They are considered safe because they have fewer side effects; however, they have low immunogenicity when used alone. We aimed to enhance the immune response of peptide-based vaccines by using self-assembled multimeric peptide amphiphiles (PAs). We designed two epitope PAs by conjugating epitope peptides from Enterovirus 71 (EV71) virus particle (VP) 1 and VP3 capsid proteins with different fatty acid chain lengths (VP1PA and VP3PA). These PAs self-assembled into supramolecular structures at a physiological pH, and the resulting structures were characterized using atomic force microscopy. Multi-epitope PAs (m-PAs) consisted of a 1:1 mixture of VP1PA and VP3PA solutions. To evaluate immunogenicity, m-PA constructs were injected with adjuvant subcutaneously into female Balb/c mice. Levels of antigen-specific immunoglobulin G (IgG) and IgG1 in m-PA-injected mice serum samples were analyzed using ELISA and Western blotting. Additionally, cytokine production stimulated by each antigen was measured in splenocytes cultured from immunized mice groups. We found that m-PA showed improved humoral and cellular immune responses compared to the control and peptide groups. The sera from m-PA immunized mice group could neutralize EV71 infection and protect host cells. Thus, self-assembled m-PAs can promote a protective immune response and can be developed as a potential platform technology to produce peptide vaccines against infectious viral diseases. MDPI 2020-11-25 /pmc/articles/PMC7760352/ /pubmed/33255791 http://dx.doi.org/10.3390/nano10122342 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Yu-Gyeong
Lee, Yunsu
Kim, Joo Hee
Chang, Sun-Young
Jung, Jong-Wha
Chung, Woo-Jae
Jin, Hyo-Eon
Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71
title Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71
title_full Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71
title_fullStr Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71
title_full_unstemmed Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71
title_short Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71
title_sort self-assembled multi-epitope peptide amphiphiles enhance the immune response against enterovirus 71
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760352/
https://www.ncbi.nlm.nih.gov/pubmed/33255791
http://dx.doi.org/10.3390/nano10122342
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