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Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage
Acinetobacter baumannii isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of A. baumannii, while, until recently, a complete genome of this strain was not available. Here, we p...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760358/ https://www.ncbi.nlm.nih.gov/pubmed/33255319 http://dx.doi.org/10.3390/microorganisms8121851 |
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author | Hamidian, Mohammad Blasco, Lucia Tillman, Lauren N. To, Joyce Tomas, María Myers, Garry S. A. |
author_facet | Hamidian, Mohammad Blasco, Lucia Tillman, Lauren N. To, Joyce Tomas, María Myers, Garry S. A. |
author_sort | Hamidian, Mohammad |
collection | PubMed |
description | Acinetobacter baumannii isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of A. baumannii, while, until recently, a complete genome of this strain was not available. Here, we present an analysis of the complete 3.91-Mbp genome sequence, generated via a combination of short-read sequencing (Illumina) and long-read sequencing (MinION), and show it contains two small cryptic plasmids and a novel complete prophage of size 41.2 kb. We also characterised several regions of the ATCC 19606 genome, leading to the identification of a novel cadmium/mercury transposon, which was named Tn6551. ATCC 19606 is an antibiotic-sensitive strain, but a comparative analysis of all publicly available ST52 strains predicts a resistance to modern antibiotics by the accumulation of antibiotic-resistance genes via plasmids in recent isolates that belong to this sequence type. |
format | Online Article Text |
id | pubmed-7760358 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77603582020-12-26 Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage Hamidian, Mohammad Blasco, Lucia Tillman, Lauren N. To, Joyce Tomas, María Myers, Garry S. A. Microorganisms Article Acinetobacter baumannii isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of A. baumannii, while, until recently, a complete genome of this strain was not available. Here, we present an analysis of the complete 3.91-Mbp genome sequence, generated via a combination of short-read sequencing (Illumina) and long-read sequencing (MinION), and show it contains two small cryptic plasmids and a novel complete prophage of size 41.2 kb. We also characterised several regions of the ATCC 19606 genome, leading to the identification of a novel cadmium/mercury transposon, which was named Tn6551. ATCC 19606 is an antibiotic-sensitive strain, but a comparative analysis of all publicly available ST52 strains predicts a resistance to modern antibiotics by the accumulation of antibiotic-resistance genes via plasmids in recent isolates that belong to this sequence type. MDPI 2020-11-24 /pmc/articles/PMC7760358/ /pubmed/33255319 http://dx.doi.org/10.3390/microorganisms8121851 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hamidian, Mohammad Blasco, Lucia Tillman, Lauren N. To, Joyce Tomas, María Myers, Garry S. A. Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage |
title | Analysis of Complete Genome Sequence of Acinetobacter
baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage |
title_full | Analysis of Complete Genome Sequence of Acinetobacter
baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage |
title_fullStr | Analysis of Complete Genome Sequence of Acinetobacter
baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage |
title_full_unstemmed | Analysis of Complete Genome Sequence of Acinetobacter
baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage |
title_short | Analysis of Complete Genome Sequence of Acinetobacter
baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage |
title_sort | analysis of complete genome sequence of acinetobacter
baumannii strain atcc 19606 reveals novel mobile genetic elements and novel prophage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760358/ https://www.ncbi.nlm.nih.gov/pubmed/33255319 http://dx.doi.org/10.3390/microorganisms8121851 |
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