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Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage

Acinetobacter baumannii isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of A. baumannii, while, until recently, a complete genome of this strain was not available. Here, we p...

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Autores principales: Hamidian, Mohammad, Blasco, Lucia, Tillman, Lauren N., To, Joyce, Tomas, María, Myers, Garry S. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760358/
https://www.ncbi.nlm.nih.gov/pubmed/33255319
http://dx.doi.org/10.3390/microorganisms8121851
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author Hamidian, Mohammad
Blasco, Lucia
Tillman, Lauren N.
To, Joyce
Tomas, María
Myers, Garry S. A.
author_facet Hamidian, Mohammad
Blasco, Lucia
Tillman, Lauren N.
To, Joyce
Tomas, María
Myers, Garry S. A.
author_sort Hamidian, Mohammad
collection PubMed
description Acinetobacter baumannii isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of A. baumannii, while, until recently, a complete genome of this strain was not available. Here, we present an analysis of the complete 3.91-Mbp genome sequence, generated via a combination of short-read sequencing (Illumina) and long-read sequencing (MinION), and show it contains two small cryptic plasmids and a novel complete prophage of size 41.2 kb. We also characterised several regions of the ATCC 19606 genome, leading to the identification of a novel cadmium/mercury transposon, which was named Tn6551. ATCC 19606 is an antibiotic-sensitive strain, but a comparative analysis of all publicly available ST52 strains predicts a resistance to modern antibiotics by the accumulation of antibiotic-resistance genes via plasmids in recent isolates that belong to this sequence type.
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spelling pubmed-77603582020-12-26 Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage Hamidian, Mohammad Blasco, Lucia Tillman, Lauren N. To, Joyce Tomas, María Myers, Garry S. A. Microorganisms Article Acinetobacter baumannii isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of A. baumannii, while, until recently, a complete genome of this strain was not available. Here, we present an analysis of the complete 3.91-Mbp genome sequence, generated via a combination of short-read sequencing (Illumina) and long-read sequencing (MinION), and show it contains two small cryptic plasmids and a novel complete prophage of size 41.2 kb. We also characterised several regions of the ATCC 19606 genome, leading to the identification of a novel cadmium/mercury transposon, which was named Tn6551. ATCC 19606 is an antibiotic-sensitive strain, but a comparative analysis of all publicly available ST52 strains predicts a resistance to modern antibiotics by the accumulation of antibiotic-resistance genes via plasmids in recent isolates that belong to this sequence type. MDPI 2020-11-24 /pmc/articles/PMC7760358/ /pubmed/33255319 http://dx.doi.org/10.3390/microorganisms8121851 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hamidian, Mohammad
Blasco, Lucia
Tillman, Lauren N.
To, Joyce
Tomas, María
Myers, Garry S. A.
Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage
title Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage
title_full Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage
title_fullStr Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage
title_full_unstemmed Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage
title_short Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage
title_sort analysis of complete genome sequence of acinetobacter baumannii strain atcc 19606 reveals novel mobile genetic elements and novel prophage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760358/
https://www.ncbi.nlm.nih.gov/pubmed/33255319
http://dx.doi.org/10.3390/microorganisms8121851
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