Cargando…

Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes

Due to the limited intrinsic healing potential of cartilage, injury to this tissue may lead to osteoarthritis. Human induced pluripotent stem cells (iPSCs), which can be differentiated into chondrocytes, are a promising source of cells for cartilage regenerative therapy. Currently, however, the meth...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Liang, Hanamatsu, Hisatoshi, Homan, Kentaro, Onodera, Tomohiro, Miyazaki, Takuji, Furukawa, Jun-ichi, Hontani, Kazutoshi, Tian, Yuan, Baba, Rikiya, Iwasaki, Norimasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760376/
https://www.ncbi.nlm.nih.gov/pubmed/33271874
http://dx.doi.org/10.3390/biom10121622
_version_ 1783627318258827264
author Xu, Liang
Hanamatsu, Hisatoshi
Homan, Kentaro
Onodera, Tomohiro
Miyazaki, Takuji
Furukawa, Jun-ichi
Hontani, Kazutoshi
Tian, Yuan
Baba, Rikiya
Iwasaki, Norimasa
author_facet Xu, Liang
Hanamatsu, Hisatoshi
Homan, Kentaro
Onodera, Tomohiro
Miyazaki, Takuji
Furukawa, Jun-ichi
Hontani, Kazutoshi
Tian, Yuan
Baba, Rikiya
Iwasaki, Norimasa
author_sort Xu, Liang
collection PubMed
description Due to the limited intrinsic healing potential of cartilage, injury to this tissue may lead to osteoarthritis. Human induced pluripotent stem cells (iPSCs), which can be differentiated into chondrocytes, are a promising source of cells for cartilage regenerative therapy. Currently, however, the methods for evaluating chondrogenic differentiation of iPSCs are very limited; the main techniques are based on the detection of chondrogenic genes and histological analysis of the extracellular matrix. The cell surface is coated with glycocalyx, a layer of glycoconjugates including glycosphingolipids (GSLs) and glycoproteins. The glycans in glycoconjugates play important roles in biological events, and their expression and structure vary widely depending on cell types and conditions. In this study, we performed a quantitative GSL-glycan analysis of human iPSCs, iPSC-derived mesenchymal stem cell like cells (iPS-MSC like cells), iPS-MSC-derived chondrocytes (iPS-MSC-CDs), bone marrow-derived mesenchymal stem cells (BMSCs), and BMSC-derived chondrocytes (BMSC-CDs) using glycoblotting technology. We found that GSL-glycan profiles differed among cell types, and that the GSL-glycome underwent a characteristic alteration during the process of chondrogenic differentiation. Furthermore, we analyzed the GSL-glycome of normal human cartilage and found that it was quite similar to that of iPS-MSC-CDs. This is the first study to evaluate GSL-glycan structures on human iPS-derived cartilaginous particles under micromass culture conditions and those of normal human cartilage. Our results indicate that GSL-glycome analysis is useful for evaluating target cell differentiation and can thus support safe regenerative medicine.
format Online
Article
Text
id pubmed-7760376
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77603762020-12-26 Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes Xu, Liang Hanamatsu, Hisatoshi Homan, Kentaro Onodera, Tomohiro Miyazaki, Takuji Furukawa, Jun-ichi Hontani, Kazutoshi Tian, Yuan Baba, Rikiya Iwasaki, Norimasa Biomolecules Article Due to the limited intrinsic healing potential of cartilage, injury to this tissue may lead to osteoarthritis. Human induced pluripotent stem cells (iPSCs), which can be differentiated into chondrocytes, are a promising source of cells for cartilage regenerative therapy. Currently, however, the methods for evaluating chondrogenic differentiation of iPSCs are very limited; the main techniques are based on the detection of chondrogenic genes and histological analysis of the extracellular matrix. The cell surface is coated with glycocalyx, a layer of glycoconjugates including glycosphingolipids (GSLs) and glycoproteins. The glycans in glycoconjugates play important roles in biological events, and their expression and structure vary widely depending on cell types and conditions. In this study, we performed a quantitative GSL-glycan analysis of human iPSCs, iPSC-derived mesenchymal stem cell like cells (iPS-MSC like cells), iPS-MSC-derived chondrocytes (iPS-MSC-CDs), bone marrow-derived mesenchymal stem cells (BMSCs), and BMSC-derived chondrocytes (BMSC-CDs) using glycoblotting technology. We found that GSL-glycan profiles differed among cell types, and that the GSL-glycome underwent a characteristic alteration during the process of chondrogenic differentiation. Furthermore, we analyzed the GSL-glycome of normal human cartilage and found that it was quite similar to that of iPS-MSC-CDs. This is the first study to evaluate GSL-glycan structures on human iPS-derived cartilaginous particles under micromass culture conditions and those of normal human cartilage. Our results indicate that GSL-glycome analysis is useful for evaluating target cell differentiation and can thus support safe regenerative medicine. MDPI 2020-12-01 /pmc/articles/PMC7760376/ /pubmed/33271874 http://dx.doi.org/10.3390/biom10121622 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Liang
Hanamatsu, Hisatoshi
Homan, Kentaro
Onodera, Tomohiro
Miyazaki, Takuji
Furukawa, Jun-ichi
Hontani, Kazutoshi
Tian, Yuan
Baba, Rikiya
Iwasaki, Norimasa
Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes
title Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes
title_full Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes
title_fullStr Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes
title_full_unstemmed Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes
title_short Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes
title_sort alterations of glycosphingolipid glycans and chondrogenic markers during differentiation of human induced pluripotent stem cells into chondrocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760376/
https://www.ncbi.nlm.nih.gov/pubmed/33271874
http://dx.doi.org/10.3390/biom10121622
work_keys_str_mv AT xuliang alterationsofglycosphingolipidglycansandchondrogenicmarkersduringdifferentiationofhumaninducedpluripotentstemcellsintochondrocytes
AT hanamatsuhisatoshi alterationsofglycosphingolipidglycansandchondrogenicmarkersduringdifferentiationofhumaninducedpluripotentstemcellsintochondrocytes
AT homankentaro alterationsofglycosphingolipidglycansandchondrogenicmarkersduringdifferentiationofhumaninducedpluripotentstemcellsintochondrocytes
AT onoderatomohiro alterationsofglycosphingolipidglycansandchondrogenicmarkersduringdifferentiationofhumaninducedpluripotentstemcellsintochondrocytes
AT miyazakitakuji alterationsofglycosphingolipidglycansandchondrogenicmarkersduringdifferentiationofhumaninducedpluripotentstemcellsintochondrocytes
AT furukawajunichi alterationsofglycosphingolipidglycansandchondrogenicmarkersduringdifferentiationofhumaninducedpluripotentstemcellsintochondrocytes
AT hontanikazutoshi alterationsofglycosphingolipidglycansandchondrogenicmarkersduringdifferentiationofhumaninducedpluripotentstemcellsintochondrocytes
AT tianyuan alterationsofglycosphingolipidglycansandchondrogenicmarkersduringdifferentiationofhumaninducedpluripotentstemcellsintochondrocytes
AT babarikiya alterationsofglycosphingolipidglycansandchondrogenicmarkersduringdifferentiationofhumaninducedpluripotentstemcellsintochondrocytes
AT iwasakinorimasa alterationsofglycosphingolipidglycansandchondrogenicmarkersduringdifferentiationofhumaninducedpluripotentstemcellsintochondrocytes