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Characterization of Novel Erwinia amylovora Jumbo Bacteriophages from Eneladusvirus Genus

Jumbo phages, which have a genome size of more than 200 kb, have recently been reported for the first time. However, limited information is available regarding their characteristics because few jumbo phages have been isolated. Therefore, in this study, we aimed to isolate and characterize other jumb...

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Autores principales: Kim, Sang Guen, Lee, Sung Bin, Giri, Sib Sankar, Kim, Hyoun Joong, Kim, Sang Wha, Kwon, Jun, Park, Jungkum, Roh, Eunjung, Park, Se Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760394/
https://www.ncbi.nlm.nih.gov/pubmed/33266226
http://dx.doi.org/10.3390/v12121373
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author Kim, Sang Guen
Lee, Sung Bin
Giri, Sib Sankar
Kim, Hyoun Joong
Kim, Sang Wha
Kwon, Jun
Park, Jungkum
Roh, Eunjung
Park, Se Chang
author_facet Kim, Sang Guen
Lee, Sung Bin
Giri, Sib Sankar
Kim, Hyoun Joong
Kim, Sang Wha
Kwon, Jun
Park, Jungkum
Roh, Eunjung
Park, Se Chang
author_sort Kim, Sang Guen
collection PubMed
description Jumbo phages, which have a genome size of more than 200 kb, have recently been reported for the first time. However, limited information is available regarding their characteristics because few jumbo phages have been isolated. Therefore, in this study, we aimed to isolate and characterize other jumbo phages. We performed comparative genomic analysis of three Erwinia phages (pEa_SNUABM_12, pEa_SNUABM_47, and pEa_SNUABM_50), each of which had a genome size of approximately 360 kb (32.5% GC content). These phages were predicted to harbor 546, 540, and 540 open reading frames with 32, 34, and 35 tRNAs, respectively. Almost all of the genes in these phages could not be functionally annotated but showed high sequence similarity with genes encoded in Serratia phage BF, a member of Eneladusvirus. The detailed comparative and phylogenetic analyses presented in this study contribute to our understanding of the diversity and evolution of Erwinia phage and the genus Eneladusvirus.
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spelling pubmed-77603942020-12-26 Characterization of Novel Erwinia amylovora Jumbo Bacteriophages from Eneladusvirus Genus Kim, Sang Guen Lee, Sung Bin Giri, Sib Sankar Kim, Hyoun Joong Kim, Sang Wha Kwon, Jun Park, Jungkum Roh, Eunjung Park, Se Chang Viruses Article Jumbo phages, which have a genome size of more than 200 kb, have recently been reported for the first time. However, limited information is available regarding their characteristics because few jumbo phages have been isolated. Therefore, in this study, we aimed to isolate and characterize other jumbo phages. We performed comparative genomic analysis of three Erwinia phages (pEa_SNUABM_12, pEa_SNUABM_47, and pEa_SNUABM_50), each of which had a genome size of approximately 360 kb (32.5% GC content). These phages were predicted to harbor 546, 540, and 540 open reading frames with 32, 34, and 35 tRNAs, respectively. Almost all of the genes in these phages could not be functionally annotated but showed high sequence similarity with genes encoded in Serratia phage BF, a member of Eneladusvirus. The detailed comparative and phylogenetic analyses presented in this study contribute to our understanding of the diversity and evolution of Erwinia phage and the genus Eneladusvirus. MDPI 2020-11-30 /pmc/articles/PMC7760394/ /pubmed/33266226 http://dx.doi.org/10.3390/v12121373 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Sang Guen
Lee, Sung Bin
Giri, Sib Sankar
Kim, Hyoun Joong
Kim, Sang Wha
Kwon, Jun
Park, Jungkum
Roh, Eunjung
Park, Se Chang
Characterization of Novel Erwinia amylovora Jumbo Bacteriophages from Eneladusvirus Genus
title Characterization of Novel Erwinia amylovora Jumbo Bacteriophages from Eneladusvirus Genus
title_full Characterization of Novel Erwinia amylovora Jumbo Bacteriophages from Eneladusvirus Genus
title_fullStr Characterization of Novel Erwinia amylovora Jumbo Bacteriophages from Eneladusvirus Genus
title_full_unstemmed Characterization of Novel Erwinia amylovora Jumbo Bacteriophages from Eneladusvirus Genus
title_short Characterization of Novel Erwinia amylovora Jumbo Bacteriophages from Eneladusvirus Genus
title_sort characterization of novel erwinia amylovora jumbo bacteriophages from eneladusvirus genus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760394/
https://www.ncbi.nlm.nih.gov/pubmed/33266226
http://dx.doi.org/10.3390/v12121373
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