Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome Editing Outcomes

[Image: see text] Prolonged Cas9 activity can hinder genome engineering as it causes off-target effects, genotoxicity, heterogeneous genome-editing outcomes, immunogenicity, and mosaicism in embryonic editing—issues which could be addressed by controlling the longevity of Cas9. Though some temporal...

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Detalles Bibliográficos
Autores principales: Sreekanth, Vedagopuram, Zhou, Qingxuan, Kokkonda, Praveen, Bermudez-Cabrera, Heysol C., Lim, Donghyun, Law, Benjamin K., Holmes, Benjamin R., Chaudhary, Santosh K., Pergu, Rajaiah, Leger, Brittany S., Walker, James A., Gifford, David K., Sherwood, Richard I., Choudhary, Amit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760466/
https://www.ncbi.nlm.nih.gov/pubmed/33376784
http://dx.doi.org/10.1021/acscentsci.0c00129

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