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Rapid and Accurate Visualization of Breast Tumors with a Fluorescent Probe Targeting α-Mannosidase 2C1
[Image: see text] Accurate detection of breast tumors and discrimination of tumor from normal tissues during breast-conserving surgery are essential to reduce the risk of misdiagnosis or recurrence. However, existing probes show substantial background signals in normal breast tissues. In this study,...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760471/ https://www.ncbi.nlm.nih.gov/pubmed/33376783 http://dx.doi.org/10.1021/acscentsci.0c01189 |
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author | Fujita, Kyohhei Kamiya, Mako Yoshioka, Takafusa Ogasawara, Akira Hino, Rumi Kojima, Ryosuke Ueo, Hiroaki Urano, Yasuteru |
author_facet | Fujita, Kyohhei Kamiya, Mako Yoshioka, Takafusa Ogasawara, Akira Hino, Rumi Kojima, Ryosuke Ueo, Hiroaki Urano, Yasuteru |
author_sort | Fujita, Kyohhei |
collection | PubMed |
description | [Image: see text] Accurate detection of breast tumors and discrimination of tumor from normal tissues during breast-conserving surgery are essential to reduce the risk of misdiagnosis or recurrence. However, existing probes show substantial background signals in normal breast tissues. In this study, we focus on glycosidase activities in breast tumors. We synthesized a series of 12 fluorescent probes and performed imaging-based evaluation on surgically resected human breast specimens. Among them, the α-mannosidase-reactive fluorescent probe HMRef-αMan detected breast cancer with 90% sensitivity and 100% specificity. We identified α-mannosidase 2C1 as the target enzyme and confirmed its overexpression in various breast tumors. We found that fibroadenoma, the most common benign breast lesion in young woman, tends to have higher α-mannosidase 2C1 activity than malignant cancer. Combined application of green-emitting HMRef-αMan and a red-emitting γ-glutamyltranspeptidase probe enabled efficient dual-color, dual-target optical discrimination of malignant and benign tumors. |
format | Online Article Text |
id | pubmed-7760471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-77604712020-12-28 Rapid and Accurate Visualization of Breast Tumors with a Fluorescent Probe Targeting α-Mannosidase 2C1 Fujita, Kyohhei Kamiya, Mako Yoshioka, Takafusa Ogasawara, Akira Hino, Rumi Kojima, Ryosuke Ueo, Hiroaki Urano, Yasuteru ACS Cent Sci [Image: see text] Accurate detection of breast tumors and discrimination of tumor from normal tissues during breast-conserving surgery are essential to reduce the risk of misdiagnosis or recurrence. However, existing probes show substantial background signals in normal breast tissues. In this study, we focus on glycosidase activities in breast tumors. We synthesized a series of 12 fluorescent probes and performed imaging-based evaluation on surgically resected human breast specimens. Among them, the α-mannosidase-reactive fluorescent probe HMRef-αMan detected breast cancer with 90% sensitivity and 100% specificity. We identified α-mannosidase 2C1 as the target enzyme and confirmed its overexpression in various breast tumors. We found that fibroadenoma, the most common benign breast lesion in young woman, tends to have higher α-mannosidase 2C1 activity than malignant cancer. Combined application of green-emitting HMRef-αMan and a red-emitting γ-glutamyltranspeptidase probe enabled efficient dual-color, dual-target optical discrimination of malignant and benign tumors. American Chemical Society 2020-10-29 2020-12-23 /pmc/articles/PMC7760471/ /pubmed/33376783 http://dx.doi.org/10.1021/acscentsci.0c01189 Text en © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Fujita, Kyohhei Kamiya, Mako Yoshioka, Takafusa Ogasawara, Akira Hino, Rumi Kojima, Ryosuke Ueo, Hiroaki Urano, Yasuteru Rapid and Accurate Visualization of Breast Tumors with a Fluorescent Probe Targeting α-Mannosidase 2C1 |
title | Rapid and Accurate Visualization of Breast Tumors
with a Fluorescent Probe Targeting α-Mannosidase 2C1 |
title_full | Rapid and Accurate Visualization of Breast Tumors
with a Fluorescent Probe Targeting α-Mannosidase 2C1 |
title_fullStr | Rapid and Accurate Visualization of Breast Tumors
with a Fluorescent Probe Targeting α-Mannosidase 2C1 |
title_full_unstemmed | Rapid and Accurate Visualization of Breast Tumors
with a Fluorescent Probe Targeting α-Mannosidase 2C1 |
title_short | Rapid and Accurate Visualization of Breast Tumors
with a Fluorescent Probe Targeting α-Mannosidase 2C1 |
title_sort | rapid and accurate visualization of breast tumors
with a fluorescent probe targeting α-mannosidase 2c1 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760471/ https://www.ncbi.nlm.nih.gov/pubmed/33376783 http://dx.doi.org/10.1021/acscentsci.0c01189 |
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