Cargando…

Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats

Type 2 diabetes mellitus, which an outcome of impaired insulin action and its secretion, is concomitantly associated with lipid abnormalities. The study was designed to evaluate the combinational effect of omega-3 fatty acids (flax and fish oil) and glibenclamide on abnormal lipid profiles, increase...

Descripción completa

Detalles Bibliográficos
Autores principales: Khadke, Suresh, Mandave, Pallavi, Kuvalekar, Aniket, Pandit, Vijaya, Karandikar, Manjiri, Mantri, Nitin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760711/
https://www.ncbi.nlm.nih.gov/pubmed/33261004
http://dx.doi.org/10.3390/nu12123652
_version_ 1783627397615058944
author Khadke, Suresh
Mandave, Pallavi
Kuvalekar, Aniket
Pandit, Vijaya
Karandikar, Manjiri
Mantri, Nitin
author_facet Khadke, Suresh
Mandave, Pallavi
Kuvalekar, Aniket
Pandit, Vijaya
Karandikar, Manjiri
Mantri, Nitin
author_sort Khadke, Suresh
collection PubMed
description Type 2 diabetes mellitus, which an outcome of impaired insulin action and its secretion, is concomitantly associated with lipid abnormalities. The study was designed to evaluate the combinational effect of omega-3 fatty acids (flax and fish oil) and glibenclamide on abnormal lipid profiles, increased blood glucose, and impaired liver and kidney functions in a high fat diet with low streptozotocin (STZ)-induced diabetic rats, including its probable mechanism of action. The male Wistar rats (n = 48) were distributed into eight groups. All animal groups except the healthy received a high fat diet (HFD) for 90 days. Further, diabetes was developed by low dose STZ (35 mg/kg). Diabetic animals received, omega-3 fatty acids (500 mg/kg), along with glibenclamide (0.25 mg/kg). Both flax and fish oil intervention decreased (p ≤ 0.001) serum triglycerides and very low density lipoprotein and elevated (p ≤ 0.001) high density lipoprotein levels in diabetic rats. Total cholesterol and low-density lipoprotein level was decreased (p ≤ 0.001) in fish oil-treated rats. However, it remained unaffected in the flax oil treatment group. Both flax and fish oil intervention downregulate the expression of fatty acid metabolism genes, transcription factors (sterol regulatory element-binding proteins-1c and nuclear factor-κβ), and their regulatory genes i.e., acetyl-coA carboxylase alpha, fatty acid synthase, and tumor necrosis factors-α. The peroxisome proliferator-activated receptor gamma gene expression was upregulated (p ≤ 0.001) in the fish oil treatment group. Whereas, carnitine palmitoyltransferase 1 and fatty acid binding protein gene expression were upregulated (p ≤ 0.001) in both flax and fish oil intervention group.
format Online
Article
Text
id pubmed-7760711
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77607112020-12-26 Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats Khadke, Suresh Mandave, Pallavi Kuvalekar, Aniket Pandit, Vijaya Karandikar, Manjiri Mantri, Nitin Nutrients Article Type 2 diabetes mellitus, which an outcome of impaired insulin action and its secretion, is concomitantly associated with lipid abnormalities. The study was designed to evaluate the combinational effect of omega-3 fatty acids (flax and fish oil) and glibenclamide on abnormal lipid profiles, increased blood glucose, and impaired liver and kidney functions in a high fat diet with low streptozotocin (STZ)-induced diabetic rats, including its probable mechanism of action. The male Wistar rats (n = 48) were distributed into eight groups. All animal groups except the healthy received a high fat diet (HFD) for 90 days. Further, diabetes was developed by low dose STZ (35 mg/kg). Diabetic animals received, omega-3 fatty acids (500 mg/kg), along with glibenclamide (0.25 mg/kg). Both flax and fish oil intervention decreased (p ≤ 0.001) serum triglycerides and very low density lipoprotein and elevated (p ≤ 0.001) high density lipoprotein levels in diabetic rats. Total cholesterol and low-density lipoprotein level was decreased (p ≤ 0.001) in fish oil-treated rats. However, it remained unaffected in the flax oil treatment group. Both flax and fish oil intervention downregulate the expression of fatty acid metabolism genes, transcription factors (sterol regulatory element-binding proteins-1c and nuclear factor-κβ), and their regulatory genes i.e., acetyl-coA carboxylase alpha, fatty acid synthase, and tumor necrosis factors-α. The peroxisome proliferator-activated receptor gamma gene expression was upregulated (p ≤ 0.001) in the fish oil treatment group. Whereas, carnitine palmitoyltransferase 1 and fatty acid binding protein gene expression were upregulated (p ≤ 0.001) in both flax and fish oil intervention group. MDPI 2020-11-27 /pmc/articles/PMC7760711/ /pubmed/33261004 http://dx.doi.org/10.3390/nu12123652 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khadke, Suresh
Mandave, Pallavi
Kuvalekar, Aniket
Pandit, Vijaya
Karandikar, Manjiri
Mantri, Nitin
Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats
title Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats
title_full Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats
title_fullStr Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats
title_full_unstemmed Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats
title_short Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats
title_sort synergistic effect of omega-3 fatty acids and oral-hypoglycemic drug on lipid normalization through modulation of hepatic gene expression in high fat diet with low streptozotocin-induced diabetic rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760711/
https://www.ncbi.nlm.nih.gov/pubmed/33261004
http://dx.doi.org/10.3390/nu12123652
work_keys_str_mv AT khadkesuresh synergisticeffectofomega3fattyacidsandoralhypoglycemicdrugonlipidnormalizationthroughmodulationofhepaticgeneexpressioninhighfatdietwithlowstreptozotocininduceddiabeticrats
AT mandavepallavi synergisticeffectofomega3fattyacidsandoralhypoglycemicdrugonlipidnormalizationthroughmodulationofhepaticgeneexpressioninhighfatdietwithlowstreptozotocininduceddiabeticrats
AT kuvalekaraniket synergisticeffectofomega3fattyacidsandoralhypoglycemicdrugonlipidnormalizationthroughmodulationofhepaticgeneexpressioninhighfatdietwithlowstreptozotocininduceddiabeticrats
AT panditvijaya synergisticeffectofomega3fattyacidsandoralhypoglycemicdrugonlipidnormalizationthroughmodulationofhepaticgeneexpressioninhighfatdietwithlowstreptozotocininduceddiabeticrats
AT karandikarmanjiri synergisticeffectofomega3fattyacidsandoralhypoglycemicdrugonlipidnormalizationthroughmodulationofhepaticgeneexpressioninhighfatdietwithlowstreptozotocininduceddiabeticrats
AT mantrinitin synergisticeffectofomega3fattyacidsandoralhypoglycemicdrugonlipidnormalizationthroughmodulationofhepaticgeneexpressioninhighfatdietwithlowstreptozotocininduceddiabeticrats