Cargando…
Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats
Type 2 diabetes mellitus, which an outcome of impaired insulin action and its secretion, is concomitantly associated with lipid abnormalities. The study was designed to evaluate the combinational effect of omega-3 fatty acids (flax and fish oil) and glibenclamide on abnormal lipid profiles, increase...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760711/ https://www.ncbi.nlm.nih.gov/pubmed/33261004 http://dx.doi.org/10.3390/nu12123652 |
_version_ | 1783627397615058944 |
---|---|
author | Khadke, Suresh Mandave, Pallavi Kuvalekar, Aniket Pandit, Vijaya Karandikar, Manjiri Mantri, Nitin |
author_facet | Khadke, Suresh Mandave, Pallavi Kuvalekar, Aniket Pandit, Vijaya Karandikar, Manjiri Mantri, Nitin |
author_sort | Khadke, Suresh |
collection | PubMed |
description | Type 2 diabetes mellitus, which an outcome of impaired insulin action and its secretion, is concomitantly associated with lipid abnormalities. The study was designed to evaluate the combinational effect of omega-3 fatty acids (flax and fish oil) and glibenclamide on abnormal lipid profiles, increased blood glucose, and impaired liver and kidney functions in a high fat diet with low streptozotocin (STZ)-induced diabetic rats, including its probable mechanism of action. The male Wistar rats (n = 48) were distributed into eight groups. All animal groups except the healthy received a high fat diet (HFD) for 90 days. Further, diabetes was developed by low dose STZ (35 mg/kg). Diabetic animals received, omega-3 fatty acids (500 mg/kg), along with glibenclamide (0.25 mg/kg). Both flax and fish oil intervention decreased (p ≤ 0.001) serum triglycerides and very low density lipoprotein and elevated (p ≤ 0.001) high density lipoprotein levels in diabetic rats. Total cholesterol and low-density lipoprotein level was decreased (p ≤ 0.001) in fish oil-treated rats. However, it remained unaffected in the flax oil treatment group. Both flax and fish oil intervention downregulate the expression of fatty acid metabolism genes, transcription factors (sterol regulatory element-binding proteins-1c and nuclear factor-κβ), and their regulatory genes i.e., acetyl-coA carboxylase alpha, fatty acid synthase, and tumor necrosis factors-α. The peroxisome proliferator-activated receptor gamma gene expression was upregulated (p ≤ 0.001) in the fish oil treatment group. Whereas, carnitine palmitoyltransferase 1 and fatty acid binding protein gene expression were upregulated (p ≤ 0.001) in both flax and fish oil intervention group. |
format | Online Article Text |
id | pubmed-7760711 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77607112020-12-26 Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats Khadke, Suresh Mandave, Pallavi Kuvalekar, Aniket Pandit, Vijaya Karandikar, Manjiri Mantri, Nitin Nutrients Article Type 2 diabetes mellitus, which an outcome of impaired insulin action and its secretion, is concomitantly associated with lipid abnormalities. The study was designed to evaluate the combinational effect of omega-3 fatty acids (flax and fish oil) and glibenclamide on abnormal lipid profiles, increased blood glucose, and impaired liver and kidney functions in a high fat diet with low streptozotocin (STZ)-induced diabetic rats, including its probable mechanism of action. The male Wistar rats (n = 48) were distributed into eight groups. All animal groups except the healthy received a high fat diet (HFD) for 90 days. Further, diabetes was developed by low dose STZ (35 mg/kg). Diabetic animals received, omega-3 fatty acids (500 mg/kg), along with glibenclamide (0.25 mg/kg). Both flax and fish oil intervention decreased (p ≤ 0.001) serum triglycerides and very low density lipoprotein and elevated (p ≤ 0.001) high density lipoprotein levels in diabetic rats. Total cholesterol and low-density lipoprotein level was decreased (p ≤ 0.001) in fish oil-treated rats. However, it remained unaffected in the flax oil treatment group. Both flax and fish oil intervention downregulate the expression of fatty acid metabolism genes, transcription factors (sterol regulatory element-binding proteins-1c and nuclear factor-κβ), and their regulatory genes i.e., acetyl-coA carboxylase alpha, fatty acid synthase, and tumor necrosis factors-α. The peroxisome proliferator-activated receptor gamma gene expression was upregulated (p ≤ 0.001) in the fish oil treatment group. Whereas, carnitine palmitoyltransferase 1 and fatty acid binding protein gene expression were upregulated (p ≤ 0.001) in both flax and fish oil intervention group. MDPI 2020-11-27 /pmc/articles/PMC7760711/ /pubmed/33261004 http://dx.doi.org/10.3390/nu12123652 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Khadke, Suresh Mandave, Pallavi Kuvalekar, Aniket Pandit, Vijaya Karandikar, Manjiri Mantri, Nitin Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats |
title | Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats |
title_full | Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats |
title_fullStr | Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats |
title_full_unstemmed | Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats |
title_short | Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats |
title_sort | synergistic effect of omega-3 fatty acids and oral-hypoglycemic drug on lipid normalization through modulation of hepatic gene expression in high fat diet with low streptozotocin-induced diabetic rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760711/ https://www.ncbi.nlm.nih.gov/pubmed/33261004 http://dx.doi.org/10.3390/nu12123652 |
work_keys_str_mv | AT khadkesuresh synergisticeffectofomega3fattyacidsandoralhypoglycemicdrugonlipidnormalizationthroughmodulationofhepaticgeneexpressioninhighfatdietwithlowstreptozotocininduceddiabeticrats AT mandavepallavi synergisticeffectofomega3fattyacidsandoralhypoglycemicdrugonlipidnormalizationthroughmodulationofhepaticgeneexpressioninhighfatdietwithlowstreptozotocininduceddiabeticrats AT kuvalekaraniket synergisticeffectofomega3fattyacidsandoralhypoglycemicdrugonlipidnormalizationthroughmodulationofhepaticgeneexpressioninhighfatdietwithlowstreptozotocininduceddiabeticrats AT panditvijaya synergisticeffectofomega3fattyacidsandoralhypoglycemicdrugonlipidnormalizationthroughmodulationofhepaticgeneexpressioninhighfatdietwithlowstreptozotocininduceddiabeticrats AT karandikarmanjiri synergisticeffectofomega3fattyacidsandoralhypoglycemicdrugonlipidnormalizationthroughmodulationofhepaticgeneexpressioninhighfatdietwithlowstreptozotocininduceddiabeticrats AT mantrinitin synergisticeffectofomega3fattyacidsandoralhypoglycemicdrugonlipidnormalizationthroughmodulationofhepaticgeneexpressioninhighfatdietwithlowstreptozotocininduceddiabeticrats |