Low Genetic Variability in Bemisia tabaci MEAM1 Populations within Farmscapes of Georgia, USA

SIMPLE SUMMARY: Sweetpotato whitefly, Bemisia tabaci Gennadius, is a serious pest of many agricultural crops worldwide. Numerous studies have examined the genetic structure of whitefly populations separated by geographical barriers; however, very few have assessed the population structure of B. tabaci at a farmscape level. A farmscape in this study is defined as heterogenous habitat with crop and non-crop areas spanning approximately 8 square kilometers. To assess the roles of farmscapes as drivers of B. tabaci genetic variation, thirty-five populations of the sweetpotato whitefly were collected from crop and non-crop plant species from fifteen farmscapes. Using mitochondrial COI gene sequences (mtCOI) and six nuclear microsatellite markers, the genetic diversity and genetic differentiation among collected B. tabaci MEAM1 populations were examined. Haplotype analysis using mtCOI sequences revealed the presence of a single B. tabaci MEAM1 haplotype across farmscapes of Georgia. Results from microsatellite markers further showed no significant genetic structuring among populations that corresponded to plant species or farmscapes from which they were collected. Annual whitefly population explosions and subsequent dispersal might have facilitated the persistence of a single panmictic B. tabaci population over all sampled farmscapes in this region. ABSTRACT: Bemisia tabaci is a whitefly species complex comprising important phloem feeding insect pests and plant virus vectors of many agricultural crops. Middle East–Asia Minor 1 (MEAM1) and Mediterranean (MED) are the two most invasive members of the B. tabaci species complex worldwide. The diversity of agroecosystems invaded by B. tabaci could potentially influence their population structure, but this has not been assessed at a farmscape level. A farmscape in this study is defined as heterogenous habitat with crop and non-crop areas spanning ~8 square kilometers. In this study, mitochondrial COI gene (mtCOI) sequences and six microsatellite markers were used to examine the population structure of B. tabaci MEAM1 colonizing different plant species at a farmscape level in Georgia, United States. Thirty-five populations of adult whiteflies on row and vegetable crops and weeds across major agricultural regions of Georgia were collected from fifteen farmscapes. Based on morphological features and mtCOI sequences, five species/cryptic species of whiteflies (B. tabaci MEAM1, B. tabaci MED, Dialeurodes citri, Trialeurodes abutiloneus, T. vaporariorum) were found. Analysis of 102 mtCOI sequences revealed the presence of a single B. tabaci MEAM1 haplotype across farmscapes in Georgia. Population genetics analyses (AMOVA, PCA and STRUCTURE) of B. tabaci MEAM1 (microsatellite data) revealed only minimal genetic differences among collected populations within and among farmscapes. Overall, our results suggest that there is a high level of gene flow among B. tabaci MEAM1 populations among farmscapes in Georgia. Frequent whitefly population explosions driven by a single or a few major whitefly-suitable hosts planted on a wide spatial scale may be the key factor behind the persistence of a single panmictic population over Georgia’s farmscapes. These population structuring effects are useful for delineating the spatial scale at which whiteflies must be managed and predicting the speed at which alleles associated with insecticide resistance might spread.