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Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis

Biomaterials, especially when coated with adhesive polymers, are a key tool for restorative medicine, being biocompatible and supportive for cell adherence, growth, and function. Aragonite skeletons of corals are biomaterials that support survival and growth of a range of cell types, including neuro...

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Autores principales: Morad, Tzachy, Hendler, Roni Mina, Canji, Eyal, Weiss, Orly Eva, Sion, Guy, Minnes, Refael, Polaq, Ania Hava Grushchenko, Merfeld, Ido, Dubinsky, Zvy, Nesher, Elimelech, Baranes, Danny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760860/
https://www.ncbi.nlm.nih.gov/pubmed/33260420
http://dx.doi.org/10.3390/polym12122850
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author Morad, Tzachy
Hendler, Roni Mina
Canji, Eyal
Weiss, Orly Eva
Sion, Guy
Minnes, Refael
Polaq, Ania Hava Grushchenko
Merfeld, Ido
Dubinsky, Zvy
Nesher, Elimelech
Baranes, Danny
author_facet Morad, Tzachy
Hendler, Roni Mina
Canji, Eyal
Weiss, Orly Eva
Sion, Guy
Minnes, Refael
Polaq, Ania Hava Grushchenko
Merfeld, Ido
Dubinsky, Zvy
Nesher, Elimelech
Baranes, Danny
author_sort Morad, Tzachy
collection PubMed
description Biomaterials, especially when coated with adhesive polymers, are a key tool for restorative medicine, being biocompatible and supportive for cell adherence, growth, and function. Aragonite skeletons of corals are biomaterials that support survival and growth of a range of cell types, including neurons and glia. However, it is not known if this scaffold affects neural cell migration or elongation of neuronal and astrocytic processes, prerequisites for initiating repair of damage in the nervous system. To address this, hippocampal cells were aggregated into neurospheres and cultivated on aragonite skeleton of the coral Trachyphyllia geoffroyi (Coral Skeleton (CS)), on naturally occurring aragonite (Geological Aragonite (GA)), and on glass, all pre-coated with the oligomer poly-D-lysine (PDL). The two aragonite matrices promoted equally strong cell migration (4.8 and 4.3-fold above glass-PDL, respectively) and axonal sprouting (1.96 and 1.95-fold above glass-PDL, respectively). However, CS-PDL had a stronger effect than GA-PDL on the promotion of astrocytic processes elongation (1.7 vs. 1.2-fold above glass-PDL, respectively) and expression of the glial fibrillary acidic protein (3.8 vs. and 1.8-fold above glass-PDL, respectively). These differences are likely to emerge from a reaction of astrocytes to the degree of roughness of the surface of the scaffold, which is higher on CS than on GA. Hence, CS-PDL and GA-PDL are scaffolds of strong capacity to derive neural cell movements and growth required for regeneration, while controlling the extent of astrocytic involvement. As such, implants of PDL-aragonites have significant potential as tools for damage repair and the reduction of scar formation in the brain following trauma or disease.
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spelling pubmed-77608602020-12-26 Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis Morad, Tzachy Hendler, Roni Mina Canji, Eyal Weiss, Orly Eva Sion, Guy Minnes, Refael Polaq, Ania Hava Grushchenko Merfeld, Ido Dubinsky, Zvy Nesher, Elimelech Baranes, Danny Polymers (Basel) Article Biomaterials, especially when coated with adhesive polymers, are a key tool for restorative medicine, being biocompatible and supportive for cell adherence, growth, and function. Aragonite skeletons of corals are biomaterials that support survival and growth of a range of cell types, including neurons and glia. However, it is not known if this scaffold affects neural cell migration or elongation of neuronal and astrocytic processes, prerequisites for initiating repair of damage in the nervous system. To address this, hippocampal cells were aggregated into neurospheres and cultivated on aragonite skeleton of the coral Trachyphyllia geoffroyi (Coral Skeleton (CS)), on naturally occurring aragonite (Geological Aragonite (GA)), and on glass, all pre-coated with the oligomer poly-D-lysine (PDL). The two aragonite matrices promoted equally strong cell migration (4.8 and 4.3-fold above glass-PDL, respectively) and axonal sprouting (1.96 and 1.95-fold above glass-PDL, respectively). However, CS-PDL had a stronger effect than GA-PDL on the promotion of astrocytic processes elongation (1.7 vs. 1.2-fold above glass-PDL, respectively) and expression of the glial fibrillary acidic protein (3.8 vs. and 1.8-fold above glass-PDL, respectively). These differences are likely to emerge from a reaction of astrocytes to the degree of roughness of the surface of the scaffold, which is higher on CS than on GA. Hence, CS-PDL and GA-PDL are scaffolds of strong capacity to derive neural cell movements and growth required for regeneration, while controlling the extent of astrocytic involvement. As such, implants of PDL-aragonites have significant potential as tools for damage repair and the reduction of scar formation in the brain following trauma or disease. MDPI 2020-11-29 /pmc/articles/PMC7760860/ /pubmed/33260420 http://dx.doi.org/10.3390/polym12122850 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Morad, Tzachy
Hendler, Roni Mina
Canji, Eyal
Weiss, Orly Eva
Sion, Guy
Minnes, Refael
Polaq, Ania Hava Grushchenko
Merfeld, Ido
Dubinsky, Zvy
Nesher, Elimelech
Baranes, Danny
Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis
title Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis
title_full Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis
title_fullStr Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis
title_full_unstemmed Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis
title_short Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis
title_sort aragonite-polylysine: neuro-regenerative scaffolds with diverse effects on astrogliosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760860/
https://www.ncbi.nlm.nih.gov/pubmed/33260420
http://dx.doi.org/10.3390/polym12122850
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