Cargando…

Apophysomyces jiangsuensis sp. nov., a Salt Tolerant and Phosphate-Solubilizing Fungus from the Tidelands of Jiangsu Province of China

A newly isolated phosphate-solubilizing fungus from the topsoil of Spartina alterniflora habitats in Yancheng coastal salt marsh was cultivated. Scanning electron microscopy observation revealed that the sporangia are nearly spherical, peach-shaped, and the spores formed on the top of sporangia. The...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Siyu, Han, Ruiming, Zhang, Huanshi, Song, Yongchun, Zhao, Fugeng, Qin, Pei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760925/
https://www.ncbi.nlm.nih.gov/pubmed/33256164
http://dx.doi.org/10.3390/microorganisms8121868
_version_ 1783627447661494272
author Li, Siyu
Han, Ruiming
Zhang, Huanshi
Song, Yongchun
Zhao, Fugeng
Qin, Pei
author_facet Li, Siyu
Han, Ruiming
Zhang, Huanshi
Song, Yongchun
Zhao, Fugeng
Qin, Pei
author_sort Li, Siyu
collection PubMed
description A newly isolated phosphate-solubilizing fungus from the topsoil of Spartina alterniflora habitats in Yancheng coastal salt marsh was cultivated. Scanning electron microscopy observation revealed that the sporangia are nearly spherical, peach-shaped, and the spores formed on the top of sporangia. The spores are ellipsoidal with raised white nubbins on the surface. Based on a polyphasic study and the genetic distance analysis referring to the sequence analysis of ITS (ITS1 + 5.8S + ITS2) and 28S rDNA (D1/D2 domains) genes, the novel species belongs to the genus Apophysomyces and is named as A. jiangsuensis. The optimum growth temperature and salinity of the new species were 28 °C and 1.15% NaCl, respectively. A study of its phosphate-solubilizing ability revealed that the fungus had an obvious decomposition effect on lecithin, Ca(3)(PO(4))(2), and AlPO(3), respectively. The pH of the fermented liquid progressively decreased from 6.85 to 2.27 after 7 days of incubation, indicating that the low molecular weight organic acids excreted into the culture liquor were oxalic, succinic, and malic acids and a trace amount of citric acid. Among these, oxalic acid was the major organic acid, and its amount reached 652.5 mg/L. These results indicated that the main mechanism underlying the dissolved phosphorus was related to the secretion of large amounts of organic acids.
format Online
Article
Text
id pubmed-7760925
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77609252020-12-26 Apophysomyces jiangsuensis sp. nov., a Salt Tolerant and Phosphate-Solubilizing Fungus from the Tidelands of Jiangsu Province of China Li, Siyu Han, Ruiming Zhang, Huanshi Song, Yongchun Zhao, Fugeng Qin, Pei Microorganisms Article A newly isolated phosphate-solubilizing fungus from the topsoil of Spartina alterniflora habitats in Yancheng coastal salt marsh was cultivated. Scanning electron microscopy observation revealed that the sporangia are nearly spherical, peach-shaped, and the spores formed on the top of sporangia. The spores are ellipsoidal with raised white nubbins on the surface. Based on a polyphasic study and the genetic distance analysis referring to the sequence analysis of ITS (ITS1 + 5.8S + ITS2) and 28S rDNA (D1/D2 domains) genes, the novel species belongs to the genus Apophysomyces and is named as A. jiangsuensis. The optimum growth temperature and salinity of the new species were 28 °C and 1.15% NaCl, respectively. A study of its phosphate-solubilizing ability revealed that the fungus had an obvious decomposition effect on lecithin, Ca(3)(PO(4))(2), and AlPO(3), respectively. The pH of the fermented liquid progressively decreased from 6.85 to 2.27 after 7 days of incubation, indicating that the low molecular weight organic acids excreted into the culture liquor were oxalic, succinic, and malic acids and a trace amount of citric acid. Among these, oxalic acid was the major organic acid, and its amount reached 652.5 mg/L. These results indicated that the main mechanism underlying the dissolved phosphorus was related to the secretion of large amounts of organic acids. MDPI 2020-11-26 /pmc/articles/PMC7760925/ /pubmed/33256164 http://dx.doi.org/10.3390/microorganisms8121868 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Siyu
Han, Ruiming
Zhang, Huanshi
Song, Yongchun
Zhao, Fugeng
Qin, Pei
Apophysomyces jiangsuensis sp. nov., a Salt Tolerant and Phosphate-Solubilizing Fungus from the Tidelands of Jiangsu Province of China
title Apophysomyces jiangsuensis sp. nov., a Salt Tolerant and Phosphate-Solubilizing Fungus from the Tidelands of Jiangsu Province of China
title_full Apophysomyces jiangsuensis sp. nov., a Salt Tolerant and Phosphate-Solubilizing Fungus from the Tidelands of Jiangsu Province of China
title_fullStr Apophysomyces jiangsuensis sp. nov., a Salt Tolerant and Phosphate-Solubilizing Fungus from the Tidelands of Jiangsu Province of China
title_full_unstemmed Apophysomyces jiangsuensis sp. nov., a Salt Tolerant and Phosphate-Solubilizing Fungus from the Tidelands of Jiangsu Province of China
title_short Apophysomyces jiangsuensis sp. nov., a Salt Tolerant and Phosphate-Solubilizing Fungus from the Tidelands of Jiangsu Province of China
title_sort apophysomyces jiangsuensis sp. nov., a salt tolerant and phosphate-solubilizing fungus from the tidelands of jiangsu province of china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760925/
https://www.ncbi.nlm.nih.gov/pubmed/33256164
http://dx.doi.org/10.3390/microorganisms8121868
work_keys_str_mv AT lisiyu apophysomycesjiangsuensisspnovasalttolerantandphosphatesolubilizingfungusfromthetidelandsofjiangsuprovinceofchina
AT hanruiming apophysomycesjiangsuensisspnovasalttolerantandphosphatesolubilizingfungusfromthetidelandsofjiangsuprovinceofchina
AT zhanghuanshi apophysomycesjiangsuensisspnovasalttolerantandphosphatesolubilizingfungusfromthetidelandsofjiangsuprovinceofchina
AT songyongchun apophysomycesjiangsuensisspnovasalttolerantandphosphatesolubilizingfungusfromthetidelandsofjiangsuprovinceofchina
AT zhaofugeng apophysomycesjiangsuensisspnovasalttolerantandphosphatesolubilizingfungusfromthetidelandsofjiangsuprovinceofchina
AT qinpei apophysomycesjiangsuensisspnovasalttolerantandphosphatesolubilizingfungusfromthetidelandsofjiangsuprovinceofchina