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Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress of Bacillus anthracis htrA-Disrupted and Parental Wild Type Strains
We previously demonstrated that the HtrA (High Temperature Requirement A) protease/chaperone active in the quality control of protein synthesis, represents an important virulence determinant of Bacillus anthracis. Virulence attenuation of htrA-disrupted Bacillus anthracis strains was attributed to s...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760947/ https://www.ncbi.nlm.nih.gov/pubmed/33265965 http://dx.doi.org/10.3390/microorganisms8121896 |
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author | Zaide, Galia Elia, Uri Cohen-Gihon, Inbar Israeli, Ma’ayan Rotem, Shahar Israeli, Ofir Ehrlich, Sharon Cohen, Hila Lazar, Shirley Beth-Din, Adi Shafferman, Avigdor Zvi, Anat Cohen, Ofer Chitlaru, Theodor |
author_facet | Zaide, Galia Elia, Uri Cohen-Gihon, Inbar Israeli, Ma’ayan Rotem, Shahar Israeli, Ofir Ehrlich, Sharon Cohen, Hila Lazar, Shirley Beth-Din, Adi Shafferman, Avigdor Zvi, Anat Cohen, Ofer Chitlaru, Theodor |
author_sort | Zaide, Galia |
collection | PubMed |
description | We previously demonstrated that the HtrA (High Temperature Requirement A) protease/chaperone active in the quality control of protein synthesis, represents an important virulence determinant of Bacillus anthracis. Virulence attenuation of htrA-disrupted Bacillus anthracis strains was attributed to susceptibility of ΔhtrA strains to stress insults, as evidenced by affected growth under various stress conditions. Here, we report a comparative RNA-seq transcriptomic study generating a database of differentially expressed genes in the B. anthracis htrA-disrupted and wild type parental strains under oxidative stress. The study demonstrates that, apart from protease and chaperone activities, HtrA exerts a regulatory role influencing expression of more than 1000 genes under stress. Functional analysis of groups or individual genes exhibiting strain-specific modulation, evidenced (i) massive downregulation in the ΔhtrA and upregulation in the WT strains of various transcriptional regulators, (ii) downregulation of translation processes in the WT strain, and (iii) downregulation of metal ion binding functions and upregulation of sporulation-associated functions in the ΔhtrA strain. These modulated functions are extensively discussed. Fifteen genes uniquely upregulated in the wild type strain were further interrogated for their modulation in response to other stress regimens. Overexpression of one of these genes, encoding for MazG (a nucleoside triphosphate pyrophosphohydrolase involved in various stress responses in other bacteria), in the ΔhtrA strain resulted in partial alleviation of the H(2)O(2)-sensitive phenotype. |
format | Online Article Text |
id | pubmed-7760947 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77609472020-12-26 Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress of Bacillus anthracis htrA-Disrupted and Parental Wild Type Strains Zaide, Galia Elia, Uri Cohen-Gihon, Inbar Israeli, Ma’ayan Rotem, Shahar Israeli, Ofir Ehrlich, Sharon Cohen, Hila Lazar, Shirley Beth-Din, Adi Shafferman, Avigdor Zvi, Anat Cohen, Ofer Chitlaru, Theodor Microorganisms Article We previously demonstrated that the HtrA (High Temperature Requirement A) protease/chaperone active in the quality control of protein synthesis, represents an important virulence determinant of Bacillus anthracis. Virulence attenuation of htrA-disrupted Bacillus anthracis strains was attributed to susceptibility of ΔhtrA strains to stress insults, as evidenced by affected growth under various stress conditions. Here, we report a comparative RNA-seq transcriptomic study generating a database of differentially expressed genes in the B. anthracis htrA-disrupted and wild type parental strains under oxidative stress. The study demonstrates that, apart from protease and chaperone activities, HtrA exerts a regulatory role influencing expression of more than 1000 genes under stress. Functional analysis of groups or individual genes exhibiting strain-specific modulation, evidenced (i) massive downregulation in the ΔhtrA and upregulation in the WT strains of various transcriptional regulators, (ii) downregulation of translation processes in the WT strain, and (iii) downregulation of metal ion binding functions and upregulation of sporulation-associated functions in the ΔhtrA strain. These modulated functions are extensively discussed. Fifteen genes uniquely upregulated in the wild type strain were further interrogated for their modulation in response to other stress regimens. Overexpression of one of these genes, encoding for MazG (a nucleoside triphosphate pyrophosphohydrolase involved in various stress responses in other bacteria), in the ΔhtrA strain resulted in partial alleviation of the H(2)O(2)-sensitive phenotype. MDPI 2020-11-30 /pmc/articles/PMC7760947/ /pubmed/33265965 http://dx.doi.org/10.3390/microorganisms8121896 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zaide, Galia Elia, Uri Cohen-Gihon, Inbar Israeli, Ma’ayan Rotem, Shahar Israeli, Ofir Ehrlich, Sharon Cohen, Hila Lazar, Shirley Beth-Din, Adi Shafferman, Avigdor Zvi, Anat Cohen, Ofer Chitlaru, Theodor Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress of Bacillus anthracis htrA-Disrupted and Parental Wild Type Strains |
title | Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress of Bacillus anthracis
htrA-Disrupted and Parental Wild Type Strains |
title_full | Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress of Bacillus anthracis
htrA-Disrupted and Parental Wild Type Strains |
title_fullStr | Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress of Bacillus anthracis
htrA-Disrupted and Parental Wild Type Strains |
title_full_unstemmed | Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress of Bacillus anthracis
htrA-Disrupted and Parental Wild Type Strains |
title_short | Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress of Bacillus anthracis
htrA-Disrupted and Parental Wild Type Strains |
title_sort | comparative analysis of the global transcriptomic response to oxidative stress of bacillus anthracis
htra-disrupted and parental wild type strains |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760947/ https://www.ncbi.nlm.nih.gov/pubmed/33265965 http://dx.doi.org/10.3390/microorganisms8121896 |
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