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HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica

Erythritol is a polyol produced by Yarrowia lipolytica under hyperosmotic stress. In this study, the osmo-sensitive strain Y. lipolytica yl-hog1Δ was subjected to stress, triggered by a high concentration of carbon sources. The strain thrived on 0.75 M erythritol medium, while the same concentration...

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Autores principales: Rzechonek, Dorota A., Szczepańczyk, Mateusz, Wang, Guokun, Borodina, Irina, Mirończuk, Aleksandra M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761004/
https://www.ncbi.nlm.nih.gov/pubmed/33261148
http://dx.doi.org/10.3390/genes11121424
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author Rzechonek, Dorota A.
Szczepańczyk, Mateusz
Wang, Guokun
Borodina, Irina
Mirończuk, Aleksandra M.
author_facet Rzechonek, Dorota A.
Szczepańczyk, Mateusz
Wang, Guokun
Borodina, Irina
Mirończuk, Aleksandra M.
author_sort Rzechonek, Dorota A.
collection PubMed
description Erythritol is a polyol produced by Yarrowia lipolytica under hyperosmotic stress. In this study, the osmo-sensitive strain Y. lipolytica yl-hog1Δ was subjected to stress, triggered by a high concentration of carbon sources. The strain thrived on 0.75 M erythritol medium, while the same concentrations of glucose and glycerol proved to be lethal. The addition of 0.1 M erythritol to the medium containing 0.75 M glucose or glycerol allowed the growth of yl-hog1Δ. Supplementation with other potential osmolytes such as mannitol or L-proline did not have a similar effect. To examine whether the osmoprotective effect might be related to erythritol accumulation, we deleted two genes involved in erythritol utilization, the transcription factor Euf1 and the enzyme erythritol dehydrogenase Eyd1. The strain eyd1Δ yl hog1Δ, which lacked the erythritol utilization enzyme, reacted to the erythritol supplementation significantly better than yl-hog1Δ. On the other hand, the strain euf1Δ yl-hog1Δ became insensitive to supplementation, and the addition of erythritol could no longer improve the growth of this strain in hyperosmotic conditions. This indicates that Euf1 regulates additional, still unknown genes involved in erythritol metabolism.
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spelling pubmed-77610042020-12-26 HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica Rzechonek, Dorota A. Szczepańczyk, Mateusz Wang, Guokun Borodina, Irina Mirończuk, Aleksandra M. Genes (Basel) Article Erythritol is a polyol produced by Yarrowia lipolytica under hyperosmotic stress. In this study, the osmo-sensitive strain Y. lipolytica yl-hog1Δ was subjected to stress, triggered by a high concentration of carbon sources. The strain thrived on 0.75 M erythritol medium, while the same concentrations of glucose and glycerol proved to be lethal. The addition of 0.1 M erythritol to the medium containing 0.75 M glucose or glycerol allowed the growth of yl-hog1Δ. Supplementation with other potential osmolytes such as mannitol or L-proline did not have a similar effect. To examine whether the osmoprotective effect might be related to erythritol accumulation, we deleted two genes involved in erythritol utilization, the transcription factor Euf1 and the enzyme erythritol dehydrogenase Eyd1. The strain eyd1Δ yl hog1Δ, which lacked the erythritol utilization enzyme, reacted to the erythritol supplementation significantly better than yl-hog1Δ. On the other hand, the strain euf1Δ yl-hog1Δ became insensitive to supplementation, and the addition of erythritol could no longer improve the growth of this strain in hyperosmotic conditions. This indicates that Euf1 regulates additional, still unknown genes involved in erythritol metabolism. MDPI 2020-11-27 /pmc/articles/PMC7761004/ /pubmed/33261148 http://dx.doi.org/10.3390/genes11121424 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rzechonek, Dorota A.
Szczepańczyk, Mateusz
Wang, Guokun
Borodina, Irina
Mirończuk, Aleksandra M.
HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica
title HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica
title_full HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica
title_fullStr HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica
title_full_unstemmed HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica
title_short HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica
title_sort hog-independent osmoprotection by erythritol in yeast yarrowia lipolytica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761004/
https://www.ncbi.nlm.nih.gov/pubmed/33261148
http://dx.doi.org/10.3390/genes11121424
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