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Fucoidans of Moroccan Brown Seaweed as Elicitors of Natural Defenses in Date Palm Roots

Fucoidans from Moroccan brown seaweed Bifurcaria bifurcata and Fucus spiralis were tested for their elicitor activity after their purification and complete characterization. The fucoidans of B. bifurcata (BBF) and of F. spiralis (FSF) were extracted and purified then characterized by infrared spectr...

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Autores principales: Bouissil, Soukaina, Alaoui-Talibi, Zainab El, Pierre, Guillaume, Rchid, Halima, Michaud, Philippe, Delattre, Cédric, El Modafar, Cherkaoui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761206/
https://www.ncbi.nlm.nih.gov/pubmed/33256188
http://dx.doi.org/10.3390/md18120596
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author Bouissil, Soukaina
Alaoui-Talibi, Zainab El
Pierre, Guillaume
Rchid, Halima
Michaud, Philippe
Delattre, Cédric
El Modafar, Cherkaoui
author_facet Bouissil, Soukaina
Alaoui-Talibi, Zainab El
Pierre, Guillaume
Rchid, Halima
Michaud, Philippe
Delattre, Cédric
El Modafar, Cherkaoui
author_sort Bouissil, Soukaina
collection PubMed
description Fucoidans from Moroccan brown seaweed Bifurcaria bifurcata and Fucus spiralis were tested for their elicitor activity after their purification and complete characterization. The fucoidans of B. bifurcata (BBF) and of F. spiralis (FSF) were extracted and purified then characterized by infrared spectroscopy, proton nuclear magnetic resonance spectroscopy and size exclusion chromatography. The results show that BBF and FSF are mainly sulfated with 45.49 and 49.53% (w/w) sulfate, respectively. Analysis of neutral sugars determined by gas chromatography–mass spectrometry showed that FSF and BBF were mainly composed of 64% and 91% fucose and 20% and 6% galactose, respectively, with a few other sugars such as glucose (8% in FSF), rhamnose (1% in BBF) and mannose (8% in FSF and, 2% in BBF). The eliciting activity of these sulfated polysaccharides in stimulating the natural defenses of the date palm was evaluated through the activity of phenylalanine ammonia-lyase (PAL), and the increase in phenols and lignin content in the roots. The results obtained clearly show that the two fucoidans early and intensely stimulate the natural defenses of the date palm after 24 h of treatments. This remarkable elicitor effect seems to be linked to the sulfated groups compared to non-sulfate alginates extracted from the same algae. These results open promising perspectives for a biological control approach against date palm diseases.
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spelling pubmed-77612062020-12-26 Fucoidans of Moroccan Brown Seaweed as Elicitors of Natural Defenses in Date Palm Roots Bouissil, Soukaina Alaoui-Talibi, Zainab El Pierre, Guillaume Rchid, Halima Michaud, Philippe Delattre, Cédric El Modafar, Cherkaoui Mar Drugs Article Fucoidans from Moroccan brown seaweed Bifurcaria bifurcata and Fucus spiralis were tested for their elicitor activity after their purification and complete characterization. The fucoidans of B. bifurcata (BBF) and of F. spiralis (FSF) were extracted and purified then characterized by infrared spectroscopy, proton nuclear magnetic resonance spectroscopy and size exclusion chromatography. The results show that BBF and FSF are mainly sulfated with 45.49 and 49.53% (w/w) sulfate, respectively. Analysis of neutral sugars determined by gas chromatography–mass spectrometry showed that FSF and BBF were mainly composed of 64% and 91% fucose and 20% and 6% galactose, respectively, with a few other sugars such as glucose (8% in FSF), rhamnose (1% in BBF) and mannose (8% in FSF and, 2% in BBF). The eliciting activity of these sulfated polysaccharides in stimulating the natural defenses of the date palm was evaluated through the activity of phenylalanine ammonia-lyase (PAL), and the increase in phenols and lignin content in the roots. The results obtained clearly show that the two fucoidans early and intensely stimulate the natural defenses of the date palm after 24 h of treatments. This remarkable elicitor effect seems to be linked to the sulfated groups compared to non-sulfate alginates extracted from the same algae. These results open promising perspectives for a biological control approach against date palm diseases. MDPI 2020-11-26 /pmc/articles/PMC7761206/ /pubmed/33256188 http://dx.doi.org/10.3390/md18120596 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bouissil, Soukaina
Alaoui-Talibi, Zainab El
Pierre, Guillaume
Rchid, Halima
Michaud, Philippe
Delattre, Cédric
El Modafar, Cherkaoui
Fucoidans of Moroccan Brown Seaweed as Elicitors of Natural Defenses in Date Palm Roots
title Fucoidans of Moroccan Brown Seaweed as Elicitors of Natural Defenses in Date Palm Roots
title_full Fucoidans of Moroccan Brown Seaweed as Elicitors of Natural Defenses in Date Palm Roots
title_fullStr Fucoidans of Moroccan Brown Seaweed as Elicitors of Natural Defenses in Date Palm Roots
title_full_unstemmed Fucoidans of Moroccan Brown Seaweed as Elicitors of Natural Defenses in Date Palm Roots
title_short Fucoidans of Moroccan Brown Seaweed as Elicitors of Natural Defenses in Date Palm Roots
title_sort fucoidans of moroccan brown seaweed as elicitors of natural defenses in date palm roots
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761206/
https://www.ncbi.nlm.nih.gov/pubmed/33256188
http://dx.doi.org/10.3390/md18120596
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