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Genome-Wide Identification of the Vacuolar H(+)-ATPase Gene Family in Five Rosaceae Species and Expression Analysis in Pear (Pyrus bretschneideri)

Vacuolar H(+)-ATPases (V-ATPase) are multi-subunit complexes that function as ATP hydrolysis-driven proton pumps. They play pivotal roles in physiological processes, such as development, metabolism, stress, and growth. However, there have been very few studies on the characterisation of V-ATPase (VH...

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Autores principales: Zhou, Hongsheng, Huang, Wen, Luo, Shufen, Hu, Huali, Zhang, Yingtong, Zhang, Leigang, Li, Pengxia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761284/
https://www.ncbi.nlm.nih.gov/pubmed/33261053
http://dx.doi.org/10.3390/plants9121661
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author Zhou, Hongsheng
Huang, Wen
Luo, Shufen
Hu, Huali
Zhang, Yingtong
Zhang, Leigang
Li, Pengxia
author_facet Zhou, Hongsheng
Huang, Wen
Luo, Shufen
Hu, Huali
Zhang, Yingtong
Zhang, Leigang
Li, Pengxia
author_sort Zhou, Hongsheng
collection PubMed
description Vacuolar H(+)-ATPases (V-ATPase) are multi-subunit complexes that function as ATP hydrolysis-driven proton pumps. They play pivotal roles in physiological processes, such as development, metabolism, stress, and growth. However, there have been very few studies on the characterisation of V-ATPase (VHA) genes in Rosaceae species. Therefore, in the present study, we performed a genome-wide analysis and identified VHA gene family members in five Rosaceae species (Pyrus bretschneideri, Malus domestica, Prunus persica, Fragaria vesca, and Prunus mume). A total of 159 VHA genes were identified, and were classified into 13 subfamilies according to the phylogenetic analysis. The structure of VHA proteins revealed high similarity among different VHA genes within the same subgroup. Gene duplication event analysis revealed that whole-genome duplications represented the major pathway for expansion of the Pyrus bretschneideri VHA genes (PbrVHA genes). The tissue-specific expression analysis of the pear showed that 36 PbrVHA genes were expressed in major tissues. Seven PbrVHA genes were significantly downregulated when the pollen tube growth stopped. Moreover, many PbrVHA genes were differentially expressed during fruit development and storage, suggesting that VHA genes play specific roles in development and senescence. The present study provides fundamental information for further elucidating the potential roles of VHA genes during development and senescence.
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spelling pubmed-77612842020-12-26 Genome-Wide Identification of the Vacuolar H(+)-ATPase Gene Family in Five Rosaceae Species and Expression Analysis in Pear (Pyrus bretschneideri) Zhou, Hongsheng Huang, Wen Luo, Shufen Hu, Huali Zhang, Yingtong Zhang, Leigang Li, Pengxia Plants (Basel) Article Vacuolar H(+)-ATPases (V-ATPase) are multi-subunit complexes that function as ATP hydrolysis-driven proton pumps. They play pivotal roles in physiological processes, such as development, metabolism, stress, and growth. However, there have been very few studies on the characterisation of V-ATPase (VHA) genes in Rosaceae species. Therefore, in the present study, we performed a genome-wide analysis and identified VHA gene family members in five Rosaceae species (Pyrus bretschneideri, Malus domestica, Prunus persica, Fragaria vesca, and Prunus mume). A total of 159 VHA genes were identified, and were classified into 13 subfamilies according to the phylogenetic analysis. The structure of VHA proteins revealed high similarity among different VHA genes within the same subgroup. Gene duplication event analysis revealed that whole-genome duplications represented the major pathway for expansion of the Pyrus bretschneideri VHA genes (PbrVHA genes). The tissue-specific expression analysis of the pear showed that 36 PbrVHA genes were expressed in major tissues. Seven PbrVHA genes were significantly downregulated when the pollen tube growth stopped. Moreover, many PbrVHA genes were differentially expressed during fruit development and storage, suggesting that VHA genes play specific roles in development and senescence. The present study provides fundamental information for further elucidating the potential roles of VHA genes during development and senescence. MDPI 2020-11-27 /pmc/articles/PMC7761284/ /pubmed/33261053 http://dx.doi.org/10.3390/plants9121661 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Hongsheng
Huang, Wen
Luo, Shufen
Hu, Huali
Zhang, Yingtong
Zhang, Leigang
Li, Pengxia
Genome-Wide Identification of the Vacuolar H(+)-ATPase Gene Family in Five Rosaceae Species and Expression Analysis in Pear (Pyrus bretschneideri)
title Genome-Wide Identification of the Vacuolar H(+)-ATPase Gene Family in Five Rosaceae Species and Expression Analysis in Pear (Pyrus bretschneideri)
title_full Genome-Wide Identification of the Vacuolar H(+)-ATPase Gene Family in Five Rosaceae Species and Expression Analysis in Pear (Pyrus bretschneideri)
title_fullStr Genome-Wide Identification of the Vacuolar H(+)-ATPase Gene Family in Five Rosaceae Species and Expression Analysis in Pear (Pyrus bretschneideri)
title_full_unstemmed Genome-Wide Identification of the Vacuolar H(+)-ATPase Gene Family in Five Rosaceae Species and Expression Analysis in Pear (Pyrus bretschneideri)
title_short Genome-Wide Identification of the Vacuolar H(+)-ATPase Gene Family in Five Rosaceae Species and Expression Analysis in Pear (Pyrus bretschneideri)
title_sort genome-wide identification of the vacuolar h(+)-atpase gene family in five rosaceae species and expression analysis in pear (pyrus bretschneideri)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761284/
https://www.ncbi.nlm.nih.gov/pubmed/33261053
http://dx.doi.org/10.3390/plants9121661
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