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Determination of Ochratoxin A and Ochratoxin B in Archived Tokaj Wines (Vintage 1959–2017) Using On-Line Solid Phase Extraction Coupled to Liquid Chromatography

According to the EU legislation, ochratoxin A contamination is controlled in wines. Tokaj wine is a special type of sweet wine produced from botrytized grapes infected by “noble rot” Botrytis cinerea. Although a high contamination was reported in sweet wines and noble rot grapes could be susceptible...

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Autores principales: Kholová, Aneta, Lhotská, Ivona, Uhrová, Adéla, Špánik, Ivan, Machyňáková, Andrea, Solich, Petr, Švec, František, Šatínský, Dalibor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761308/
https://www.ncbi.nlm.nih.gov/pubmed/33255273
http://dx.doi.org/10.3390/toxins12120739
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author Kholová, Aneta
Lhotská, Ivona
Uhrová, Adéla
Špánik, Ivan
Machyňáková, Andrea
Solich, Petr
Švec, František
Šatínský, Dalibor
author_facet Kholová, Aneta
Lhotská, Ivona
Uhrová, Adéla
Špánik, Ivan
Machyňáková, Andrea
Solich, Petr
Švec, František
Šatínský, Dalibor
author_sort Kholová, Aneta
collection PubMed
description According to the EU legislation, ochratoxin A contamination is controlled in wines. Tokaj wine is a special type of sweet wine produced from botrytized grapes infected by “noble rot” Botrytis cinerea. Although a high contamination was reported in sweet wines and noble rot grapes could be susceptible to coinfection with other fungi, including ochratoxigenic species, no screening of Tokaj wines for mycotoxin contamination has been carried out so far. Therefore, we developed an analytical method for the determination of ochratoxin A (OTA) and ochratoxin B (OTB) involving online SPE coupled to HPLC-FD using column switching to achieve the fast and sensitive control of mycotoxin contamination. The method was validated with recoveries ranging from 91.6% to 99.1% with an RSD less than 2%. The limits of quantification were 0.1 and 0.2 µg L(−1) for OTA and OTB, respectively. The total analysis time of the online SPE-HPLC-FD method was a mere 6 min. This high throughput enables routine analysis. Finally, we carried out an extensive investigation of the ochratoxin contamination in 59 Slovak Tokaj wines of 1959–2017 vintage. Only a few positives were detected. The OTA content in most of the checked wines did not exceed the EU maximum tolerable limit of 2 µg L(−1), indicating a good quality of winegrowing and storing.
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spelling pubmed-77613082020-12-26 Determination of Ochratoxin A and Ochratoxin B in Archived Tokaj Wines (Vintage 1959–2017) Using On-Line Solid Phase Extraction Coupled to Liquid Chromatography Kholová, Aneta Lhotská, Ivona Uhrová, Adéla Špánik, Ivan Machyňáková, Andrea Solich, Petr Švec, František Šatínský, Dalibor Toxins (Basel) Article According to the EU legislation, ochratoxin A contamination is controlled in wines. Tokaj wine is a special type of sweet wine produced from botrytized grapes infected by “noble rot” Botrytis cinerea. Although a high contamination was reported in sweet wines and noble rot grapes could be susceptible to coinfection with other fungi, including ochratoxigenic species, no screening of Tokaj wines for mycotoxin contamination has been carried out so far. Therefore, we developed an analytical method for the determination of ochratoxin A (OTA) and ochratoxin B (OTB) involving online SPE coupled to HPLC-FD using column switching to achieve the fast and sensitive control of mycotoxin contamination. The method was validated with recoveries ranging from 91.6% to 99.1% with an RSD less than 2%. The limits of quantification were 0.1 and 0.2 µg L(−1) for OTA and OTB, respectively. The total analysis time of the online SPE-HPLC-FD method was a mere 6 min. This high throughput enables routine analysis. Finally, we carried out an extensive investigation of the ochratoxin contamination in 59 Slovak Tokaj wines of 1959–2017 vintage. Only a few positives were detected. The OTA content in most of the checked wines did not exceed the EU maximum tolerable limit of 2 µg L(−1), indicating a good quality of winegrowing and storing. MDPI 2020-11-24 /pmc/articles/PMC7761308/ /pubmed/33255273 http://dx.doi.org/10.3390/toxins12120739 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kholová, Aneta
Lhotská, Ivona
Uhrová, Adéla
Špánik, Ivan
Machyňáková, Andrea
Solich, Petr
Švec, František
Šatínský, Dalibor
Determination of Ochratoxin A and Ochratoxin B in Archived Tokaj Wines (Vintage 1959–2017) Using On-Line Solid Phase Extraction Coupled to Liquid Chromatography
title Determination of Ochratoxin A and Ochratoxin B in Archived Tokaj Wines (Vintage 1959–2017) Using On-Line Solid Phase Extraction Coupled to Liquid Chromatography
title_full Determination of Ochratoxin A and Ochratoxin B in Archived Tokaj Wines (Vintage 1959–2017) Using On-Line Solid Phase Extraction Coupled to Liquid Chromatography
title_fullStr Determination of Ochratoxin A and Ochratoxin B in Archived Tokaj Wines (Vintage 1959–2017) Using On-Line Solid Phase Extraction Coupled to Liquid Chromatography
title_full_unstemmed Determination of Ochratoxin A and Ochratoxin B in Archived Tokaj Wines (Vintage 1959–2017) Using On-Line Solid Phase Extraction Coupled to Liquid Chromatography
title_short Determination of Ochratoxin A and Ochratoxin B in Archived Tokaj Wines (Vintage 1959–2017) Using On-Line Solid Phase Extraction Coupled to Liquid Chromatography
title_sort determination of ochratoxin a and ochratoxin b in archived tokaj wines (vintage 1959–2017) using on-line solid phase extraction coupled to liquid chromatography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761308/
https://www.ncbi.nlm.nih.gov/pubmed/33255273
http://dx.doi.org/10.3390/toxins12120739
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