Cargando…

Multiple Introductions and Predominance of Rotavirus Group A Genotype G3P[8] in Kilifi, Coastal Kenya, 4 Years after Nationwide Vaccine Introduction

Globally, rotavirus group A (RVA) remains a major cause of severe childhood diarrhea, despite the use of vaccines in more than 100 countries. RVA sequencing for local outbreaks facilitates investigation into strain composition, origins, spread, and vaccine failure. In 2018, we collected 248 stool sa...

Descripción completa

Detalles Bibliográficos
Autores principales: Mwanga, Mike J., Verani, Jennifer R., Omore, Richard, Tate, Jacqueline E., Parashar, Umesh D., Murunga, Nickson, Gicheru, Elijah, Breiman, Robert F., Nokes, D. James, Agoti, Charles N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761311/
https://www.ncbi.nlm.nih.gov/pubmed/33255256
http://dx.doi.org/10.3390/pathogens9120981
_version_ 1783627539001901056
author Mwanga, Mike J.
Verani, Jennifer R.
Omore, Richard
Tate, Jacqueline E.
Parashar, Umesh D.
Murunga, Nickson
Gicheru, Elijah
Breiman, Robert F.
Nokes, D. James
Agoti, Charles N.
author_facet Mwanga, Mike J.
Verani, Jennifer R.
Omore, Richard
Tate, Jacqueline E.
Parashar, Umesh D.
Murunga, Nickson
Gicheru, Elijah
Breiman, Robert F.
Nokes, D. James
Agoti, Charles N.
author_sort Mwanga, Mike J.
collection PubMed
description Globally, rotavirus group A (RVA) remains a major cause of severe childhood diarrhea, despite the use of vaccines in more than 100 countries. RVA sequencing for local outbreaks facilitates investigation into strain composition, origins, spread, and vaccine failure. In 2018, we collected 248 stool samples from children aged less than 13 years admitted with diarrheal illness to Kilifi County Hospital, coastal Kenya. Antigen screening detected RVA in 55 samples (22.2%). Of these, VP7 (G) and VP4 (P) segments were successfully sequenced in 48 (87.3%) and phylogenetic analysis based on the VP7 sequences identified seven genetic clusters with six different GP combinations: G3P[8], G1P[8], G2P[4], G2P[8], G9P[8] and G12P[8]. The G3P[8] strains predominated the season (n = 37, 67.2%) and comprised three distinct G3 genetic clusters that fell within Lineage I and IX (the latter also known as equine-like G3 Lineage). Both the two G3 lineages have been recently detected in several countries. Our study is the first to document African children infected with G3 Lineage IX. These data highlight the global nature of RVA transmission and the importance of increasing global rotavirus vaccine coverage.
format Online
Article
Text
id pubmed-7761311
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77613112020-12-26 Multiple Introductions and Predominance of Rotavirus Group A Genotype G3P[8] in Kilifi, Coastal Kenya, 4 Years after Nationwide Vaccine Introduction Mwanga, Mike J. Verani, Jennifer R. Omore, Richard Tate, Jacqueline E. Parashar, Umesh D. Murunga, Nickson Gicheru, Elijah Breiman, Robert F. Nokes, D. James Agoti, Charles N. Pathogens Article Globally, rotavirus group A (RVA) remains a major cause of severe childhood diarrhea, despite the use of vaccines in more than 100 countries. RVA sequencing for local outbreaks facilitates investigation into strain composition, origins, spread, and vaccine failure. In 2018, we collected 248 stool samples from children aged less than 13 years admitted with diarrheal illness to Kilifi County Hospital, coastal Kenya. Antigen screening detected RVA in 55 samples (22.2%). Of these, VP7 (G) and VP4 (P) segments were successfully sequenced in 48 (87.3%) and phylogenetic analysis based on the VP7 sequences identified seven genetic clusters with six different GP combinations: G3P[8], G1P[8], G2P[4], G2P[8], G9P[8] and G12P[8]. The G3P[8] strains predominated the season (n = 37, 67.2%) and comprised three distinct G3 genetic clusters that fell within Lineage I and IX (the latter also known as equine-like G3 Lineage). Both the two G3 lineages have been recently detected in several countries. Our study is the first to document African children infected with G3 Lineage IX. These data highlight the global nature of RVA transmission and the importance of increasing global rotavirus vaccine coverage. MDPI 2020-11-24 /pmc/articles/PMC7761311/ /pubmed/33255256 http://dx.doi.org/10.3390/pathogens9120981 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mwanga, Mike J.
Verani, Jennifer R.
Omore, Richard
Tate, Jacqueline E.
Parashar, Umesh D.
Murunga, Nickson
Gicheru, Elijah
Breiman, Robert F.
Nokes, D. James
Agoti, Charles N.
Multiple Introductions and Predominance of Rotavirus Group A Genotype G3P[8] in Kilifi, Coastal Kenya, 4 Years after Nationwide Vaccine Introduction
title Multiple Introductions and Predominance of Rotavirus Group A Genotype G3P[8] in Kilifi, Coastal Kenya, 4 Years after Nationwide Vaccine Introduction
title_full Multiple Introductions and Predominance of Rotavirus Group A Genotype G3P[8] in Kilifi, Coastal Kenya, 4 Years after Nationwide Vaccine Introduction
title_fullStr Multiple Introductions and Predominance of Rotavirus Group A Genotype G3P[8] in Kilifi, Coastal Kenya, 4 Years after Nationwide Vaccine Introduction
title_full_unstemmed Multiple Introductions and Predominance of Rotavirus Group A Genotype G3P[8] in Kilifi, Coastal Kenya, 4 Years after Nationwide Vaccine Introduction
title_short Multiple Introductions and Predominance of Rotavirus Group A Genotype G3P[8] in Kilifi, Coastal Kenya, 4 Years after Nationwide Vaccine Introduction
title_sort multiple introductions and predominance of rotavirus group a genotype g3p[8] in kilifi, coastal kenya, 4 years after nationwide vaccine introduction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761311/
https://www.ncbi.nlm.nih.gov/pubmed/33255256
http://dx.doi.org/10.3390/pathogens9120981
work_keys_str_mv AT mwangamikej multipleintroductionsandpredominanceofrotavirusgroupagenotypeg3p8inkilificoastalkenya4yearsafternationwidevaccineintroduction
AT veranijenniferr multipleintroductionsandpredominanceofrotavirusgroupagenotypeg3p8inkilificoastalkenya4yearsafternationwidevaccineintroduction
AT omorerichard multipleintroductionsandpredominanceofrotavirusgroupagenotypeg3p8inkilificoastalkenya4yearsafternationwidevaccineintroduction
AT tatejacquelinee multipleintroductionsandpredominanceofrotavirusgroupagenotypeg3p8inkilificoastalkenya4yearsafternationwidevaccineintroduction
AT parasharumeshd multipleintroductionsandpredominanceofrotavirusgroupagenotypeg3p8inkilificoastalkenya4yearsafternationwidevaccineintroduction
AT murunganickson multipleintroductionsandpredominanceofrotavirusgroupagenotypeg3p8inkilificoastalkenya4yearsafternationwidevaccineintroduction
AT gicheruelijah multipleintroductionsandpredominanceofrotavirusgroupagenotypeg3p8inkilificoastalkenya4yearsafternationwidevaccineintroduction
AT breimanrobertf multipleintroductionsandpredominanceofrotavirusgroupagenotypeg3p8inkilificoastalkenya4yearsafternationwidevaccineintroduction
AT nokesdjames multipleintroductionsandpredominanceofrotavirusgroupagenotypeg3p8inkilificoastalkenya4yearsafternationwidevaccineintroduction
AT agoticharlesn multipleintroductionsandpredominanceofrotavirusgroupagenotypeg3p8inkilificoastalkenya4yearsafternationwidevaccineintroduction