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New Mitochondrial Gene Rearrangement in Psyttalia concolor, P. humilis and P. lounsburyi (Hymenoptera: Braconidae), Three Parasitoid Species of Economic Interest
SIMPLE SUMMARY: Parasitoid wasps in the family Braconidae are generally highly specialized and can be used as agents for biological control of arthropod pests. Psyttalia concolor, Psyttalia humilis and Psyttalia lounsburyi parasitize the larvae of the olive fruit fly (Bactrocera oleae), the most dam...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761351/ https://www.ncbi.nlm.nih.gov/pubmed/33276418 http://dx.doi.org/10.3390/insects11120854 |
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author | Powell, Chanté Caleca, Virgilio Rhode, Clint Teixeira da Costa, Luis van Asch, Barbara |
author_facet | Powell, Chanté Caleca, Virgilio Rhode, Clint Teixeira da Costa, Luis van Asch, Barbara |
author_sort | Powell, Chanté |
collection | PubMed |
description | SIMPLE SUMMARY: Parasitoid wasps in the family Braconidae are generally highly specialized and can be used as agents for biological control of arthropod pests. Psyttalia concolor, Psyttalia humilis and Psyttalia lounsburyi parasitize the larvae of the olive fruit fly (Bactrocera oleae), the most damaging pest of cultivated olives in the world. Psyttalia concolor is native to the Mediterranean, and P. humilis and P. lounsburyi are native to sub-Saharan Africa. Despite their potential for agricultural pest control, these species have been poorly characterized at the genetic level. We sequenced the mitochondrial genome of the three species and compared its organization with other Braconidae. Psyttalia had a unique gene rearrangement involving the positions of transfer RNA genes. We also present a phylogenetic reconstruction of the Braconidae and confirm the phylogenetic placement of Psyttalia in the subfamily Opiinae. ABSTRACT: The family Braconidae consists mostly of specialized parasitoids, some of which hold potential in biocontrol of agricultural pests. Psyttalia concolor, Psyttalia humilis and Psyttalia lounsburyi are parasitoids associated with Bactrocera oleae, a major pest of cultivated olives. The native range of Psyttalia concolor is the Mediterranean, and P. humilis and P. lounsburyi are native to sub-Saharan Africa. This study reports the mitochondrial genomes of the three species, thus laying the foundation for mitogenomic analyses in the genus Psyttalia. Comparative mitogenomics within Braconidae showed a novel gene arrangement in Psyttalia in involving translocation and inversion of transfer RNA genes. The placement of Psyttalia in the subfamily Opiinae was well-supported, and the divergence between Psyttalia and its closest relative (Diachasmimorpha longicaudata) was at ~55 MYA [95% highest posterior density (HPD): 34–83 MYA]. Psyttalia lounsburyi occupied the most basal position among the three Psyttalia, having diverged from the other two species ~11 MYA (95% HPD: 6–17 MYA). Psyttalia concolor and P. humilis were recovered as sister species diverged at ~2 MYA (95% HPD: 1.1–3.6 MYA). This phylogeny combining new sequences and a set of 31 other cyclostomes and non-cyclostomes highlights the importance of a comprehensive taxonomic coverage of Braconidae mitogenomes to overcome the lack of robustness in the placement of several subfamilies. |
format | Online Article Text |
id | pubmed-7761351 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77613512020-12-26 New Mitochondrial Gene Rearrangement in Psyttalia concolor, P. humilis and P. lounsburyi (Hymenoptera: Braconidae), Three Parasitoid Species of Economic Interest Powell, Chanté Caleca, Virgilio Rhode, Clint Teixeira da Costa, Luis van Asch, Barbara Insects Article SIMPLE SUMMARY: Parasitoid wasps in the family Braconidae are generally highly specialized and can be used as agents for biological control of arthropod pests. Psyttalia concolor, Psyttalia humilis and Psyttalia lounsburyi parasitize the larvae of the olive fruit fly (Bactrocera oleae), the most damaging pest of cultivated olives in the world. Psyttalia concolor is native to the Mediterranean, and P. humilis and P. lounsburyi are native to sub-Saharan Africa. Despite their potential for agricultural pest control, these species have been poorly characterized at the genetic level. We sequenced the mitochondrial genome of the three species and compared its organization with other Braconidae. Psyttalia had a unique gene rearrangement involving the positions of transfer RNA genes. We also present a phylogenetic reconstruction of the Braconidae and confirm the phylogenetic placement of Psyttalia in the subfamily Opiinae. ABSTRACT: The family Braconidae consists mostly of specialized parasitoids, some of which hold potential in biocontrol of agricultural pests. Psyttalia concolor, Psyttalia humilis and Psyttalia lounsburyi are parasitoids associated with Bactrocera oleae, a major pest of cultivated olives. The native range of Psyttalia concolor is the Mediterranean, and P. humilis and P. lounsburyi are native to sub-Saharan Africa. This study reports the mitochondrial genomes of the three species, thus laying the foundation for mitogenomic analyses in the genus Psyttalia. Comparative mitogenomics within Braconidae showed a novel gene arrangement in Psyttalia in involving translocation and inversion of transfer RNA genes. The placement of Psyttalia in the subfamily Opiinae was well-supported, and the divergence between Psyttalia and its closest relative (Diachasmimorpha longicaudata) was at ~55 MYA [95% highest posterior density (HPD): 34–83 MYA]. Psyttalia lounsburyi occupied the most basal position among the three Psyttalia, having diverged from the other two species ~11 MYA (95% HPD: 6–17 MYA). Psyttalia concolor and P. humilis were recovered as sister species diverged at ~2 MYA (95% HPD: 1.1–3.6 MYA). This phylogeny combining new sequences and a set of 31 other cyclostomes and non-cyclostomes highlights the importance of a comprehensive taxonomic coverage of Braconidae mitogenomes to overcome the lack of robustness in the placement of several subfamilies. MDPI 2020-12-02 /pmc/articles/PMC7761351/ /pubmed/33276418 http://dx.doi.org/10.3390/insects11120854 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Powell, Chanté Caleca, Virgilio Rhode, Clint Teixeira da Costa, Luis van Asch, Barbara New Mitochondrial Gene Rearrangement in Psyttalia concolor, P. humilis and P. lounsburyi (Hymenoptera: Braconidae), Three Parasitoid Species of Economic Interest |
title | New Mitochondrial Gene Rearrangement in Psyttalia concolor, P. humilis and P. lounsburyi (Hymenoptera: Braconidae), Three Parasitoid Species of Economic Interest |
title_full | New Mitochondrial Gene Rearrangement in Psyttalia concolor, P. humilis and P. lounsburyi (Hymenoptera: Braconidae), Three Parasitoid Species of Economic Interest |
title_fullStr | New Mitochondrial Gene Rearrangement in Psyttalia concolor, P. humilis and P. lounsburyi (Hymenoptera: Braconidae), Three Parasitoid Species of Economic Interest |
title_full_unstemmed | New Mitochondrial Gene Rearrangement in Psyttalia concolor, P. humilis and P. lounsburyi (Hymenoptera: Braconidae), Three Parasitoid Species of Economic Interest |
title_short | New Mitochondrial Gene Rearrangement in Psyttalia concolor, P. humilis and P. lounsburyi (Hymenoptera: Braconidae), Three Parasitoid Species of Economic Interest |
title_sort | new mitochondrial gene rearrangement in psyttalia concolor, p. humilis and p. lounsburyi (hymenoptera: braconidae), three parasitoid species of economic interest |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761351/ https://www.ncbi.nlm.nih.gov/pubmed/33276418 http://dx.doi.org/10.3390/insects11120854 |
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