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FDM 3D Printed Composites for Bone Tissue Engineering Based on Plasticized Poly(3-hydroxybutyrate)/poly(d,l-lactide) Blends

Tissue engineering is a current trend in the regenerative medicine putting pressure on scientists to develop highly functional materials and methods for scaffolds’ preparation. In this paper, the calibrated filaments for Fused Deposition Modeling (FDM) based on plasticized poly(3-hydroxybutyrate)/po...

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Autores principales: Melčová, Veronika, Svoradová, Kateřina, Menčík, Přemysl, Kontárová, Soňa, Rampichová, Michala, Hedvičáková, Věra, Sovková, Věra, Přikryl, Radek, Vojtová, Lucy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761374/
https://www.ncbi.nlm.nih.gov/pubmed/33260879
http://dx.doi.org/10.3390/polym12122806
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author Melčová, Veronika
Svoradová, Kateřina
Menčík, Přemysl
Kontárová, Soňa
Rampichová, Michala
Hedvičáková, Věra
Sovková, Věra
Přikryl, Radek
Vojtová, Lucy
author_facet Melčová, Veronika
Svoradová, Kateřina
Menčík, Přemysl
Kontárová, Soňa
Rampichová, Michala
Hedvičáková, Věra
Sovková, Věra
Přikryl, Radek
Vojtová, Lucy
author_sort Melčová, Veronika
collection PubMed
description Tissue engineering is a current trend in the regenerative medicine putting pressure on scientists to develop highly functional materials and methods for scaffolds’ preparation. In this paper, the calibrated filaments for Fused Deposition Modeling (FDM) based on plasticized poly(3-hydroxybutyrate)/poly(d,l-lactide) 70/30 blend modified with tricalcium phosphate bioceramics were prepared. Two different plasticizers, Citroflex (n-Butyryl tri-n-hexyl citrate) and Syncroflex (oligomeric adipate ester), both used in the amount of 12 wt%, were compared. The printing parameters for these materials were optimized and the printability was evaluated by recently published warping test. The samples were studied with respect to their thermal and mechanical properties, followed by biological in vitro tests including proliferation, viability, and osteogenic differentiation of human mesenchymal stem cells. According to the results from differential scanning calorimetry and tensile measurements, the Citroflex-based plasticizer showed very good softening effect at the expense of worse printability and unsatisfactory performance during biological testing. On the other hand, the samples with Syncroflex demonstrated lower warping tendency compared to commercial polylactide filament with the warping coefficient one third lower. Moreover, the Syncroflex-based samples exhibited the non-cytotoxicity and promising biocompatibility.
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spelling pubmed-77613742020-12-26 FDM 3D Printed Composites for Bone Tissue Engineering Based on Plasticized Poly(3-hydroxybutyrate)/poly(d,l-lactide) Blends Melčová, Veronika Svoradová, Kateřina Menčík, Přemysl Kontárová, Soňa Rampichová, Michala Hedvičáková, Věra Sovková, Věra Přikryl, Radek Vojtová, Lucy Polymers (Basel) Article Tissue engineering is a current trend in the regenerative medicine putting pressure on scientists to develop highly functional materials and methods for scaffolds’ preparation. In this paper, the calibrated filaments for Fused Deposition Modeling (FDM) based on plasticized poly(3-hydroxybutyrate)/poly(d,l-lactide) 70/30 blend modified with tricalcium phosphate bioceramics were prepared. Two different plasticizers, Citroflex (n-Butyryl tri-n-hexyl citrate) and Syncroflex (oligomeric adipate ester), both used in the amount of 12 wt%, were compared. The printing parameters for these materials were optimized and the printability was evaluated by recently published warping test. The samples were studied with respect to their thermal and mechanical properties, followed by biological in vitro tests including proliferation, viability, and osteogenic differentiation of human mesenchymal stem cells. According to the results from differential scanning calorimetry and tensile measurements, the Citroflex-based plasticizer showed very good softening effect at the expense of worse printability and unsatisfactory performance during biological testing. On the other hand, the samples with Syncroflex demonstrated lower warping tendency compared to commercial polylactide filament with the warping coefficient one third lower. Moreover, the Syncroflex-based samples exhibited the non-cytotoxicity and promising biocompatibility. MDPI 2020-11-27 /pmc/articles/PMC7761374/ /pubmed/33260879 http://dx.doi.org/10.3390/polym12122806 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Melčová, Veronika
Svoradová, Kateřina
Menčík, Přemysl
Kontárová, Soňa
Rampichová, Michala
Hedvičáková, Věra
Sovková, Věra
Přikryl, Radek
Vojtová, Lucy
FDM 3D Printed Composites for Bone Tissue Engineering Based on Plasticized Poly(3-hydroxybutyrate)/poly(d,l-lactide) Blends
title FDM 3D Printed Composites for Bone Tissue Engineering Based on Plasticized Poly(3-hydroxybutyrate)/poly(d,l-lactide) Blends
title_full FDM 3D Printed Composites for Bone Tissue Engineering Based on Plasticized Poly(3-hydroxybutyrate)/poly(d,l-lactide) Blends
title_fullStr FDM 3D Printed Composites for Bone Tissue Engineering Based on Plasticized Poly(3-hydroxybutyrate)/poly(d,l-lactide) Blends
title_full_unstemmed FDM 3D Printed Composites for Bone Tissue Engineering Based on Plasticized Poly(3-hydroxybutyrate)/poly(d,l-lactide) Blends
title_short FDM 3D Printed Composites for Bone Tissue Engineering Based on Plasticized Poly(3-hydroxybutyrate)/poly(d,l-lactide) Blends
title_sort fdm 3d printed composites for bone tissue engineering based on plasticized poly(3-hydroxybutyrate)/poly(d,l-lactide) blends
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761374/
https://www.ncbi.nlm.nih.gov/pubmed/33260879
http://dx.doi.org/10.3390/polym12122806
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