How Have Leukocyte In Vitro Chemotaxis Assays Shaped Our Ideas about Macrophage Migration?

SIMPLE SUMMARY: The migration of immune cells is vital during inflammatory responses. Macrophages, which are a subset of immune cells, are unique in the ways they migrate because they can switch between different mechanism of migration. This crucial feature of macrophage migration has been underappreciated in the literature because technologies used to study macrophage migration were not able to efficiently detect those subtle differences between macrophages and other immune cells. This review article describes popular technologies used to study macrophage migration and critically assesses their advantages and disadvantages in macrophage migration studies. ABSTRACT: Macrophage chemotaxis is crucial during both onset and resolution of inflammation and unique among all leukocytes. Macrophages are able to switch between amoeboid and mesenchymal migration to optimise their migration through 3D environments. This subtle migration phenotype has been underappreciated in the literature, with macrophages often being grouped and discussed together with other leukocytes, possibly due to the limitations of current chemotaxis assays. Transwell assays were originally designed in the 1960s but despite their long-known limitations, they are still one of the most popular methods of studying macrophage migration. This review aims to critically evaluate transwell assays, and other popular chemotaxis assays, comparing their advantages and limitations in macrophage migration studies.