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The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression
Low-grade gliomas (LGG) are infiltrative primary brain tumors that in 70% of the cases undergo anaplastic transformation, deeply affecting prognosis. However, the timing of progression is heterogeneous. Recently, the tumor microenvironment (TME) has gained much attention either as prognostic factor...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761654/ https://www.ncbi.nlm.nih.gov/pubmed/33287106 http://dx.doi.org/10.3390/biomedicines8120564 |
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author | Caponnetto, Federica Dalla, Emiliano Mangoni, Damiano Piazza, Silvano Radovic, Slobodanka Ius, Tamara Skrap, Miran Di Loreto, Carla Beltrami, Antonio Paolo Manini, Ivana Cesselli, Daniela |
author_facet | Caponnetto, Federica Dalla, Emiliano Mangoni, Damiano Piazza, Silvano Radovic, Slobodanka Ius, Tamara Skrap, Miran Di Loreto, Carla Beltrami, Antonio Paolo Manini, Ivana Cesselli, Daniela |
author_sort | Caponnetto, Federica |
collection | PubMed |
description | Low-grade gliomas (LGG) are infiltrative primary brain tumors that in 70% of the cases undergo anaplastic transformation, deeply affecting prognosis. However, the timing of progression is heterogeneous. Recently, the tumor microenvironment (TME) has gained much attention either as prognostic factor or therapeutic target. Through the release of extracellular vesicles, the TME contributes to tumor progression by transferring bioactive molecules such as microRNA. The aim of the study was to take advantage of glioma-associated stem cells (GASC), an in vitro model of the glioma microenvironment endowed with a prognostic significance, and their released exosomes, to investigate the possible role of exosome miRNAs in favoring the anaplastic transformation of LGG. Therefore, by deep sequencing, we analyzed and compared the miRNA profile of GASC and exosomes obtained from LGG patients characterized by different prognosis. Results showed that exosomes presented a different signature, when compared to their cellular counterpart and that, although sharing several miRNAs, exosomes of patients with a bad prognosis, selectively expressed some miRNAs possibly responsible for the more aggressive phenotype. These findings get insights into the value of TME and exosomes as potential biomarkers for precision medicine approaches aimed at improving LGG prognostic stratification and therapeutic strategies. |
format | Online Article Text |
id | pubmed-7761654 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77616542020-12-26 The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression Caponnetto, Federica Dalla, Emiliano Mangoni, Damiano Piazza, Silvano Radovic, Slobodanka Ius, Tamara Skrap, Miran Di Loreto, Carla Beltrami, Antonio Paolo Manini, Ivana Cesselli, Daniela Biomedicines Article Low-grade gliomas (LGG) are infiltrative primary brain tumors that in 70% of the cases undergo anaplastic transformation, deeply affecting prognosis. However, the timing of progression is heterogeneous. Recently, the tumor microenvironment (TME) has gained much attention either as prognostic factor or therapeutic target. Through the release of extracellular vesicles, the TME contributes to tumor progression by transferring bioactive molecules such as microRNA. The aim of the study was to take advantage of glioma-associated stem cells (GASC), an in vitro model of the glioma microenvironment endowed with a prognostic significance, and their released exosomes, to investigate the possible role of exosome miRNAs in favoring the anaplastic transformation of LGG. Therefore, by deep sequencing, we analyzed and compared the miRNA profile of GASC and exosomes obtained from LGG patients characterized by different prognosis. Results showed that exosomes presented a different signature, when compared to their cellular counterpart and that, although sharing several miRNAs, exosomes of patients with a bad prognosis, selectively expressed some miRNAs possibly responsible for the more aggressive phenotype. These findings get insights into the value of TME and exosomes as potential biomarkers for precision medicine approaches aimed at improving LGG prognostic stratification and therapeutic strategies. MDPI 2020-12-03 /pmc/articles/PMC7761654/ /pubmed/33287106 http://dx.doi.org/10.3390/biomedicines8120564 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Caponnetto, Federica Dalla, Emiliano Mangoni, Damiano Piazza, Silvano Radovic, Slobodanka Ius, Tamara Skrap, Miran Di Loreto, Carla Beltrami, Antonio Paolo Manini, Ivana Cesselli, Daniela The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression |
title | The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression |
title_full | The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression |
title_fullStr | The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression |
title_full_unstemmed | The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression |
title_short | The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression |
title_sort | mirna content of exosomes released from the glioma microenvironment can affect malignant progression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761654/ https://www.ncbi.nlm.nih.gov/pubmed/33287106 http://dx.doi.org/10.3390/biomedicines8120564 |
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