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The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression

Low-grade gliomas (LGG) are infiltrative primary brain tumors that in 70% of the cases undergo anaplastic transformation, deeply affecting prognosis. However, the timing of progression is heterogeneous. Recently, the tumor microenvironment (TME) has gained much attention either as prognostic factor...

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Autores principales: Caponnetto, Federica, Dalla, Emiliano, Mangoni, Damiano, Piazza, Silvano, Radovic, Slobodanka, Ius, Tamara, Skrap, Miran, Di Loreto, Carla, Beltrami, Antonio Paolo, Manini, Ivana, Cesselli, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761654/
https://www.ncbi.nlm.nih.gov/pubmed/33287106
http://dx.doi.org/10.3390/biomedicines8120564
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author Caponnetto, Federica
Dalla, Emiliano
Mangoni, Damiano
Piazza, Silvano
Radovic, Slobodanka
Ius, Tamara
Skrap, Miran
Di Loreto, Carla
Beltrami, Antonio Paolo
Manini, Ivana
Cesselli, Daniela
author_facet Caponnetto, Federica
Dalla, Emiliano
Mangoni, Damiano
Piazza, Silvano
Radovic, Slobodanka
Ius, Tamara
Skrap, Miran
Di Loreto, Carla
Beltrami, Antonio Paolo
Manini, Ivana
Cesselli, Daniela
author_sort Caponnetto, Federica
collection PubMed
description Low-grade gliomas (LGG) are infiltrative primary brain tumors that in 70% of the cases undergo anaplastic transformation, deeply affecting prognosis. However, the timing of progression is heterogeneous. Recently, the tumor microenvironment (TME) has gained much attention either as prognostic factor or therapeutic target. Through the release of extracellular vesicles, the TME contributes to tumor progression by transferring bioactive molecules such as microRNA. The aim of the study was to take advantage of glioma-associated stem cells (GASC), an in vitro model of the glioma microenvironment endowed with a prognostic significance, and their released exosomes, to investigate the possible role of exosome miRNAs in favoring the anaplastic transformation of LGG. Therefore, by deep sequencing, we analyzed and compared the miRNA profile of GASC and exosomes obtained from LGG patients characterized by different prognosis. Results showed that exosomes presented a different signature, when compared to their cellular counterpart and that, although sharing several miRNAs, exosomes of patients with a bad prognosis, selectively expressed some miRNAs possibly responsible for the more aggressive phenotype. These findings get insights into the value of TME and exosomes as potential biomarkers for precision medicine approaches aimed at improving LGG prognostic stratification and therapeutic strategies.
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spelling pubmed-77616542020-12-26 The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression Caponnetto, Federica Dalla, Emiliano Mangoni, Damiano Piazza, Silvano Radovic, Slobodanka Ius, Tamara Skrap, Miran Di Loreto, Carla Beltrami, Antonio Paolo Manini, Ivana Cesselli, Daniela Biomedicines Article Low-grade gliomas (LGG) are infiltrative primary brain tumors that in 70% of the cases undergo anaplastic transformation, deeply affecting prognosis. However, the timing of progression is heterogeneous. Recently, the tumor microenvironment (TME) has gained much attention either as prognostic factor or therapeutic target. Through the release of extracellular vesicles, the TME contributes to tumor progression by transferring bioactive molecules such as microRNA. The aim of the study was to take advantage of glioma-associated stem cells (GASC), an in vitro model of the glioma microenvironment endowed with a prognostic significance, and their released exosomes, to investigate the possible role of exosome miRNAs in favoring the anaplastic transformation of LGG. Therefore, by deep sequencing, we analyzed and compared the miRNA profile of GASC and exosomes obtained from LGG patients characterized by different prognosis. Results showed that exosomes presented a different signature, when compared to their cellular counterpart and that, although sharing several miRNAs, exosomes of patients with a bad prognosis, selectively expressed some miRNAs possibly responsible for the more aggressive phenotype. These findings get insights into the value of TME and exosomes as potential biomarkers for precision medicine approaches aimed at improving LGG prognostic stratification and therapeutic strategies. MDPI 2020-12-03 /pmc/articles/PMC7761654/ /pubmed/33287106 http://dx.doi.org/10.3390/biomedicines8120564 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Caponnetto, Federica
Dalla, Emiliano
Mangoni, Damiano
Piazza, Silvano
Radovic, Slobodanka
Ius, Tamara
Skrap, Miran
Di Loreto, Carla
Beltrami, Antonio Paolo
Manini, Ivana
Cesselli, Daniela
The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression
title The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression
title_full The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression
title_fullStr The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression
title_full_unstemmed The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression
title_short The miRNA Content of Exosomes Released from the Glioma Microenvironment Can Affect Malignant Progression
title_sort mirna content of exosomes released from the glioma microenvironment can affect malignant progression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761654/
https://www.ncbi.nlm.nih.gov/pubmed/33287106
http://dx.doi.org/10.3390/biomedicines8120564
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