Cargando…

First Report of the Production of Mycotoxins and Other Secondary Metabolites by Macrophomina phaseolina (Tassi) Goid. Isolates from Soybeans (Glycine max L.) Symptomatic with Charcoal Rot Disease

Macrophomina phaseolina (Tassi) Goid., the causal agent of charcoal rot disease of soybean, is capable of causing disease in more than 500 other commercially important plants. This fungus produces several secondary metabolites in culture, including (-)-botryodiplodin, phaseolinone and mellein. Given...

Descripción completa

Detalles Bibliográficos
Autores principales: Khambhati, Vivek H., Abbas, Hamed K., Sulyok, Michael, Tomaso-Peterson, Maria, Shier, W. Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761776/
https://www.ncbi.nlm.nih.gov/pubmed/33287215
http://dx.doi.org/10.3390/jof6040332
_version_ 1783627647888130048
author Khambhati, Vivek H.
Abbas, Hamed K.
Sulyok, Michael
Tomaso-Peterson, Maria
Shier, W. Thomas
author_facet Khambhati, Vivek H.
Abbas, Hamed K.
Sulyok, Michael
Tomaso-Peterson, Maria
Shier, W. Thomas
author_sort Khambhati, Vivek H.
collection PubMed
description Macrophomina phaseolina (Tassi) Goid., the causal agent of charcoal rot disease of soybean, is capable of causing disease in more than 500 other commercially important plants. This fungus produces several secondary metabolites in culture, including (-)-botryodiplodin, phaseolinone and mellein. Given that independent fungal isolates may differ in mycotoxin and secondary metabolite production, we examined a collection of 89 independent M. phaseolina isolates from soybean plants with charcoal rot disease using LC-MS/MS analysis of culture filtrates. In addition to (-)-botryodiplodin and mellein, four previously unreported metabolites were observed in >19% of cultures, including kojic acid (84.3% of cultures at 0.57–79.9 µg/L), moniliformin (61.8% of cultures at 0.011–12.9 µg/L), orsellinic acid (49.4% of cultures at 5.71–1960 µg/L) and cyclo[L-proline-L-tyrosine] (19.1% of cultures at 0.012–0.082 µg/L). In addition, nine previously unreported metabolites were observed at a substantially lower frequency (<5% of cultures), including cordycepin, emodin, endocrocin, citrinin, gliocladic acid, infectopyron, methylorsellinic acid, monocerin and N-benzoyl-L-phenylalanine. Further studies are needed to investigate the possible effects of these mycotoxins and metabolites on pathogenesis by M. phaseolina and on food and feed safety, if any of them contaminate the seeds of infected soybean plants.
format Online
Article
Text
id pubmed-7761776
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77617762020-12-26 First Report of the Production of Mycotoxins and Other Secondary Metabolites by Macrophomina phaseolina (Tassi) Goid. Isolates from Soybeans (Glycine max L.) Symptomatic with Charcoal Rot Disease Khambhati, Vivek H. Abbas, Hamed K. Sulyok, Michael Tomaso-Peterson, Maria Shier, W. Thomas J Fungi (Basel) Brief Report Macrophomina phaseolina (Tassi) Goid., the causal agent of charcoal rot disease of soybean, is capable of causing disease in more than 500 other commercially important plants. This fungus produces several secondary metabolites in culture, including (-)-botryodiplodin, phaseolinone and mellein. Given that independent fungal isolates may differ in mycotoxin and secondary metabolite production, we examined a collection of 89 independent M. phaseolina isolates from soybean plants with charcoal rot disease using LC-MS/MS analysis of culture filtrates. In addition to (-)-botryodiplodin and mellein, four previously unreported metabolites were observed in >19% of cultures, including kojic acid (84.3% of cultures at 0.57–79.9 µg/L), moniliformin (61.8% of cultures at 0.011–12.9 µg/L), orsellinic acid (49.4% of cultures at 5.71–1960 µg/L) and cyclo[L-proline-L-tyrosine] (19.1% of cultures at 0.012–0.082 µg/L). In addition, nine previously unreported metabolites were observed at a substantially lower frequency (<5% of cultures), including cordycepin, emodin, endocrocin, citrinin, gliocladic acid, infectopyron, methylorsellinic acid, monocerin and N-benzoyl-L-phenylalanine. Further studies are needed to investigate the possible effects of these mycotoxins and metabolites on pathogenesis by M. phaseolina and on food and feed safety, if any of them contaminate the seeds of infected soybean plants. MDPI 2020-12-03 /pmc/articles/PMC7761776/ /pubmed/33287215 http://dx.doi.org/10.3390/jof6040332 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Khambhati, Vivek H.
Abbas, Hamed K.
Sulyok, Michael
Tomaso-Peterson, Maria
Shier, W. Thomas
First Report of the Production of Mycotoxins and Other Secondary Metabolites by Macrophomina phaseolina (Tassi) Goid. Isolates from Soybeans (Glycine max L.) Symptomatic with Charcoal Rot Disease
title First Report of the Production of Mycotoxins and Other Secondary Metabolites by Macrophomina phaseolina (Tassi) Goid. Isolates from Soybeans (Glycine max L.) Symptomatic with Charcoal Rot Disease
title_full First Report of the Production of Mycotoxins and Other Secondary Metabolites by Macrophomina phaseolina (Tassi) Goid. Isolates from Soybeans (Glycine max L.) Symptomatic with Charcoal Rot Disease
title_fullStr First Report of the Production of Mycotoxins and Other Secondary Metabolites by Macrophomina phaseolina (Tassi) Goid. Isolates from Soybeans (Glycine max L.) Symptomatic with Charcoal Rot Disease
title_full_unstemmed First Report of the Production of Mycotoxins and Other Secondary Metabolites by Macrophomina phaseolina (Tassi) Goid. Isolates from Soybeans (Glycine max L.) Symptomatic with Charcoal Rot Disease
title_short First Report of the Production of Mycotoxins and Other Secondary Metabolites by Macrophomina phaseolina (Tassi) Goid. Isolates from Soybeans (Glycine max L.) Symptomatic with Charcoal Rot Disease
title_sort first report of the production of mycotoxins and other secondary metabolites by macrophomina phaseolina (tassi) goid. isolates from soybeans (glycine max l.) symptomatic with charcoal rot disease
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761776/
https://www.ncbi.nlm.nih.gov/pubmed/33287215
http://dx.doi.org/10.3390/jof6040332
work_keys_str_mv AT khambhativivekh firstreportoftheproductionofmycotoxinsandothersecondarymetabolitesbymacrophominaphaseolinatassigoidisolatesfromsoybeansglycinemaxlsymptomaticwithcharcoalrotdisease
AT abbashamedk firstreportoftheproductionofmycotoxinsandothersecondarymetabolitesbymacrophominaphaseolinatassigoidisolatesfromsoybeansglycinemaxlsymptomaticwithcharcoalrotdisease
AT sulyokmichael firstreportoftheproductionofmycotoxinsandothersecondarymetabolitesbymacrophominaphaseolinatassigoidisolatesfromsoybeansglycinemaxlsymptomaticwithcharcoalrotdisease
AT tomasopetersonmaria firstreportoftheproductionofmycotoxinsandothersecondarymetabolitesbymacrophominaphaseolinatassigoidisolatesfromsoybeansglycinemaxlsymptomaticwithcharcoalrotdisease
AT shierwthomas firstreportoftheproductionofmycotoxinsandothersecondarymetabolitesbymacrophominaphaseolinatassigoidisolatesfromsoybeansglycinemaxlsymptomaticwithcharcoalrotdisease