Cargando…
Presence of Cutaneous Complement Deposition Distinguishes between Immunological and Histological Features of Bullous Pemphigoid—Insights from a Retrospective Cohort Study
The practical implications of complement deposition in direct immunofluorescence (DIF) microscopy and its influence on the disease phenotype are poorly understood. We aimed to investigate whether the presence of complement deposition in DIF microscopy gives rise to differences in the morphological,...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761814/ https://www.ncbi.nlm.nih.gov/pubmed/33287364 http://dx.doi.org/10.3390/jcm9123928 |
_version_ | 1783627656284078080 |
---|---|
author | Ständer, Sascha Holtsche, Maike M. Schmidt, Enno Hammers, Christoph M. Zillikens, Detlef Ludwig, Ralf J. Kridin, Khalaf |
author_facet | Ständer, Sascha Holtsche, Maike M. Schmidt, Enno Hammers, Christoph M. Zillikens, Detlef Ludwig, Ralf J. Kridin, Khalaf |
author_sort | Ständer, Sascha |
collection | PubMed |
description | The practical implications of complement deposition in direct immunofluorescence (DIF) microscopy and its influence on the disease phenotype are poorly understood. We aimed to investigate whether the presence of complement deposition in DIF microscopy gives rise to differences in the morphological, immunological, and histological characteristics of patients with BP (bullous pemphigoid). We performed a retrospective study encompassing patients with BP in a specialized tertiary referral center. Logistic regression model was utilized to identify variables independently associated with complement deposition. The study included 233 patients with BP, of whom 196 (84.1%) demonstrated linear C3 deposition along the dermal-epidermal junction (DEJ) in DIF analysis. BP patients with C3 deposition had higher mean (SD) levels (645.2 (1418.5) vs. 172.5 (243.9) U/mL; p < 0.001) and seropositivity rate (86.3% vs.64.9%; p = 0.002) of anti-BP180 NC16A and less prevalent neutrophilic infiltrate in lesional skin specimens (29.8% vs. 52.4%; p = 0.041). C3 deposition was found positively associated with the detection of anti-BP180 NC16A autoantibodies (OR, 4.25; 95% CI, 1.38–13.05) and inversely associated with the presence of neutrophils in lesional skin (OR, 3.03; 95% CI, 1.09–8.33). To conclude, complement deposition influences the immunological and histological features of BP. These findings are in line with experimental data describing the pathogenic role of complement in BP. |
format | Online Article Text |
id | pubmed-7761814 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77618142020-12-26 Presence of Cutaneous Complement Deposition Distinguishes between Immunological and Histological Features of Bullous Pemphigoid—Insights from a Retrospective Cohort Study Ständer, Sascha Holtsche, Maike M. Schmidt, Enno Hammers, Christoph M. Zillikens, Detlef Ludwig, Ralf J. Kridin, Khalaf J Clin Med Article The practical implications of complement deposition in direct immunofluorescence (DIF) microscopy and its influence on the disease phenotype are poorly understood. We aimed to investigate whether the presence of complement deposition in DIF microscopy gives rise to differences in the morphological, immunological, and histological characteristics of patients with BP (bullous pemphigoid). We performed a retrospective study encompassing patients with BP in a specialized tertiary referral center. Logistic regression model was utilized to identify variables independently associated with complement deposition. The study included 233 patients with BP, of whom 196 (84.1%) demonstrated linear C3 deposition along the dermal-epidermal junction (DEJ) in DIF analysis. BP patients with C3 deposition had higher mean (SD) levels (645.2 (1418.5) vs. 172.5 (243.9) U/mL; p < 0.001) and seropositivity rate (86.3% vs.64.9%; p = 0.002) of anti-BP180 NC16A and less prevalent neutrophilic infiltrate in lesional skin specimens (29.8% vs. 52.4%; p = 0.041). C3 deposition was found positively associated with the detection of anti-BP180 NC16A autoantibodies (OR, 4.25; 95% CI, 1.38–13.05) and inversely associated with the presence of neutrophils in lesional skin (OR, 3.03; 95% CI, 1.09–8.33). To conclude, complement deposition influences the immunological and histological features of BP. These findings are in line with experimental data describing the pathogenic role of complement in BP. MDPI 2020-12-03 /pmc/articles/PMC7761814/ /pubmed/33287364 http://dx.doi.org/10.3390/jcm9123928 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ständer, Sascha Holtsche, Maike M. Schmidt, Enno Hammers, Christoph M. Zillikens, Detlef Ludwig, Ralf J. Kridin, Khalaf Presence of Cutaneous Complement Deposition Distinguishes between Immunological and Histological Features of Bullous Pemphigoid—Insights from a Retrospective Cohort Study |
title | Presence of Cutaneous Complement Deposition Distinguishes between Immunological and Histological Features of Bullous Pemphigoid—Insights from a Retrospective Cohort Study |
title_full | Presence of Cutaneous Complement Deposition Distinguishes between Immunological and Histological Features of Bullous Pemphigoid—Insights from a Retrospective Cohort Study |
title_fullStr | Presence of Cutaneous Complement Deposition Distinguishes between Immunological and Histological Features of Bullous Pemphigoid—Insights from a Retrospective Cohort Study |
title_full_unstemmed | Presence of Cutaneous Complement Deposition Distinguishes between Immunological and Histological Features of Bullous Pemphigoid—Insights from a Retrospective Cohort Study |
title_short | Presence of Cutaneous Complement Deposition Distinguishes between Immunological and Histological Features of Bullous Pemphigoid—Insights from a Retrospective Cohort Study |
title_sort | presence of cutaneous complement deposition distinguishes between immunological and histological features of bullous pemphigoid—insights from a retrospective cohort study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761814/ https://www.ncbi.nlm.nih.gov/pubmed/33287364 http://dx.doi.org/10.3390/jcm9123928 |
work_keys_str_mv | AT standersascha presenceofcutaneouscomplementdepositiondistinguishesbetweenimmunologicalandhistologicalfeaturesofbullouspemphigoidinsightsfromaretrospectivecohortstudy AT holtschemaikem presenceofcutaneouscomplementdepositiondistinguishesbetweenimmunologicalandhistologicalfeaturesofbullouspemphigoidinsightsfromaretrospectivecohortstudy AT schmidtenno presenceofcutaneouscomplementdepositiondistinguishesbetweenimmunologicalandhistologicalfeaturesofbullouspemphigoidinsightsfromaretrospectivecohortstudy AT hammerschristophm presenceofcutaneouscomplementdepositiondistinguishesbetweenimmunologicalandhistologicalfeaturesofbullouspemphigoidinsightsfromaretrospectivecohortstudy AT zillikensdetlef presenceofcutaneouscomplementdepositiondistinguishesbetweenimmunologicalandhistologicalfeaturesofbullouspemphigoidinsightsfromaretrospectivecohortstudy AT ludwigralfj presenceofcutaneouscomplementdepositiondistinguishesbetweenimmunologicalandhistologicalfeaturesofbullouspemphigoidinsightsfromaretrospectivecohortstudy AT kridinkhalaf presenceofcutaneouscomplementdepositiondistinguishesbetweenimmunologicalandhistologicalfeaturesofbullouspemphigoidinsightsfromaretrospectivecohortstudy |