Selective Breeding for Low and High Varroa destructor Growth in Honey Bee (Apis mellifera) Colonies: Initial Results of Two Generations

SIMPLE SUMMARY: The mite Varroa destructor is considered the most damaging parasite of honey bees worldwide. Beekeepers use synthetic chemical products to control mite infestations in colonies, but the parasites soon develop resistance to them, which compromises their control. One alternative control strategy is the development of Varroa-resistant honey bees. Therefore, a breeding program was initiated to select for lower and higher rates of Varroa-population growth (LVG and HVG, respectively) and deformed wing virus (DWV) levels, which is transmitted by the mites. After two years of bidirectional selection, LVG colonies had Varroa population increases over the summer of 1.7 fold compared to 9.6 fold for HVG colonies. Additionally, HVG colonies had higher mite infestation rates in adult bees compared to LVG colonies. DWV presence and levels were higher in HVG colonies than in LVG colonies and winter mortality rates were 26% and 14% for the HVG and LVG bee types, respectively. The results of this study thus far indicate that selection for LVG may result in colonies with lower Varroa infestation rates, lower prevalence, and levels of DWV and higher colony winter survivorship. Future work will focus on determining mechanisms responsible for genetic differences and in identifying genes associated with Varroa-resistance in honey bees. ABSTRACT: After two years of bidirectional selection for low and high rates of Varroa destructor population growth (LVG and HVG, respectively) in honey bee (Apis mellifera) colonies in Ontario, Canada, significant differences between the two genotypes were observed. LVG colonies had V. destructor population increases over the summer of 1.7 fold compared to 9.6 fold for HVG colonies by Generation 2. Additionally, HVG colonies had significantly higher mite infestation rates in adult bees compared to LVG colonies for both selected generations. DWV prevalence and levels were significantly higher in HVG colonies than in LVG colonies in Generation 1 but not in Generation 2. Winter mortality rates of Generation 1 colonies were significantly different at 26% and 14% for the HVG and LVG genotypes, respectively. The results of this study thus far indicate that selection for LVG may result in colonies with lower V. destructor infestation rates, lower prevalence, and levels of DWV and higher colony winter survivorship. Future work will focus on determining what mechanisms are responsible for the genotypic differences, estimating genetic parameters, and molecular analyses of the genotypes to identify candidate genes associated with resistance to V. destructor and DWV that could potentially be used for marker-assisted selection.