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Proteasome Biology: Chemistry and Bioengineering Insights

Proteasomal degradation provides the crucial machinery for maintaining cellular proteostasis. The biological origins of modulation or impairment of the function of proteasomal complexes may include changes in gene expression of their subunits, ubiquitin mutation, or indirect mechanisms arising from...

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Detalles Bibliográficos
Autores principales: Račková, Lucia, Csekes, Erika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761984/
https://www.ncbi.nlm.nih.gov/pubmed/33291646
http://dx.doi.org/10.3390/polym12122909
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author Račková, Lucia
Csekes, Erika
author_facet Račková, Lucia
Csekes, Erika
author_sort Račková, Lucia
collection PubMed
description Proteasomal degradation provides the crucial machinery for maintaining cellular proteostasis. The biological origins of modulation or impairment of the function of proteasomal complexes may include changes in gene expression of their subunits, ubiquitin mutation, or indirect mechanisms arising from the overall impairment of proteostasis. However, changes in the physico-chemical characteristics of the cellular environment might also meaningfully contribute to altered performance. This review summarizes the effects of physicochemical factors in the cell, such as pH, temperature fluctuations, and reactions with the products of oxidative metabolism, on the function of the proteasome. Furthermore, evidence of the direct interaction of proteasomal complexes with protein aggregates is compared against the knowledge obtained from immobilization biotechnologies. In this regard, factors such as the structures of the natural polymeric scaffolds in the cells, their content of reactive groups or the sequestration of metal ions, and processes at the interface, are discussed here with regard to their influences on proteasomal function.
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spelling pubmed-77619842020-12-26 Proteasome Biology: Chemistry and Bioengineering Insights Račková, Lucia Csekes, Erika Polymers (Basel) Review Proteasomal degradation provides the crucial machinery for maintaining cellular proteostasis. The biological origins of modulation or impairment of the function of proteasomal complexes may include changes in gene expression of their subunits, ubiquitin mutation, or indirect mechanisms arising from the overall impairment of proteostasis. However, changes in the physico-chemical characteristics of the cellular environment might also meaningfully contribute to altered performance. This review summarizes the effects of physicochemical factors in the cell, such as pH, temperature fluctuations, and reactions with the products of oxidative metabolism, on the function of the proteasome. Furthermore, evidence of the direct interaction of proteasomal complexes with protein aggregates is compared against the knowledge obtained from immobilization biotechnologies. In this regard, factors such as the structures of the natural polymeric scaffolds in the cells, their content of reactive groups or the sequestration of metal ions, and processes at the interface, are discussed here with regard to their influences on proteasomal function. MDPI 2020-12-04 /pmc/articles/PMC7761984/ /pubmed/33291646 http://dx.doi.org/10.3390/polym12122909 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Račková, Lucia
Csekes, Erika
Proteasome Biology: Chemistry and Bioengineering Insights
title Proteasome Biology: Chemistry and Bioengineering Insights
title_full Proteasome Biology: Chemistry and Bioengineering Insights
title_fullStr Proteasome Biology: Chemistry and Bioengineering Insights
title_full_unstemmed Proteasome Biology: Chemistry and Bioengineering Insights
title_short Proteasome Biology: Chemistry and Bioengineering Insights
title_sort proteasome biology: chemistry and bioengineering insights
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761984/
https://www.ncbi.nlm.nih.gov/pubmed/33291646
http://dx.doi.org/10.3390/polym12122909
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