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Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure

The aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical rele...

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Autores principales: Vodicka, Josef, Pesta, Martin, Kulda, Vlastimil, Houfkova, Katerina, Vankova, Bohuslava, Sebek, Jakub, Skala, Martin, Fichtl, Jakub, Prochazkova, Kristyna, Topolcan, Ondrej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762050/
https://www.ncbi.nlm.nih.gov/pubmed/33291819
http://dx.doi.org/10.3390/cells9122611
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author Vodicka, Josef
Pesta, Martin
Kulda, Vlastimil
Houfkova, Katerina
Vankova, Bohuslava
Sebek, Jakub
Skala, Martin
Fichtl, Jakub
Prochazkova, Kristyna
Topolcan, Ondrej
author_facet Vodicka, Josef
Pesta, Martin
Kulda, Vlastimil
Houfkova, Katerina
Vankova, Bohuslava
Sebek, Jakub
Skala, Martin
Fichtl, Jakub
Prochazkova, Kristyna
Topolcan, Ondrej
author_sort Vodicka, Josef
collection PubMed
description The aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical relevance of the results was evaluated based on relation to prognosis; the disease-free interval (DFI) and overall survival (OS) were analyzed. During radical surgical treatment, a total of 1426 lymph nodes were obtained from 100 patients, creating 472 groups of nodes (4–5 groups per patient) and examined by H&E, IHC CK19 and OSNA. The median follow-up was 44 months. Concordant results on the lymph node status of the H&E, IHC CK19 and OSNA examinations were reported in 78% of patients. We recorded shorter OS in patients with positive results provided by both OSNA and H&E. The study demonstrated a higher percentage of detected micrometastases in lymph nodes by the OSNA method. However, the higher sensitivity of the OSNA, with the cut-off value 250 copies of mRNA of CK19/µL, resulted in a lower association of OSNA positivity with progress of the disease compared to H&E. Increasing the cut-off to 615 copies resulted in an increase in concordance between the OSNA and H&E, which means that the higher cut-off is more relevant in the case of lung tumors.
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spelling pubmed-77620502020-12-26 Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure Vodicka, Josef Pesta, Martin Kulda, Vlastimil Houfkova, Katerina Vankova, Bohuslava Sebek, Jakub Skala, Martin Fichtl, Jakub Prochazkova, Kristyna Topolcan, Ondrej Cells Article The aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical relevance of the results was evaluated based on relation to prognosis; the disease-free interval (DFI) and overall survival (OS) were analyzed. During radical surgical treatment, a total of 1426 lymph nodes were obtained from 100 patients, creating 472 groups of nodes (4–5 groups per patient) and examined by H&E, IHC CK19 and OSNA. The median follow-up was 44 months. Concordant results on the lymph node status of the H&E, IHC CK19 and OSNA examinations were reported in 78% of patients. We recorded shorter OS in patients with positive results provided by both OSNA and H&E. The study demonstrated a higher percentage of detected micrometastases in lymph nodes by the OSNA method. However, the higher sensitivity of the OSNA, with the cut-off value 250 copies of mRNA of CK19/µL, resulted in a lower association of OSNA positivity with progress of the disease compared to H&E. Increasing the cut-off to 615 copies resulted in an increase in concordance between the OSNA and H&E, which means that the higher cut-off is more relevant in the case of lung tumors. MDPI 2020-12-04 /pmc/articles/PMC7762050/ /pubmed/33291819 http://dx.doi.org/10.3390/cells9122611 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vodicka, Josef
Pesta, Martin
Kulda, Vlastimil
Houfkova, Katerina
Vankova, Bohuslava
Sebek, Jakub
Skala, Martin
Fichtl, Jakub
Prochazkova, Kristyna
Topolcan, Ondrej
Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure
title Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure
title_full Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure
title_fullStr Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure
title_full_unstemmed Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure
title_short Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure
title_sort prognostic significance of lymph node examination by the osna method in lung cancer patients—comparison with the standard histopathological procedure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762050/
https://www.ncbi.nlm.nih.gov/pubmed/33291819
http://dx.doi.org/10.3390/cells9122611
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