Dietary Fatty Acids Change Circulating Fatty Acids, Microbial Putrefactive Postbiotics and Betaine Status in the Cat

SIMPLE SUMMARY: The cat is an obligate carnivore that is well adapted to dietary polyunsaturated fatty acids (PUFA), perhaps because of the variance resulting from normal consumption of organ meat which is high in PUFA, and storage lipid which is often relatively low in PUFA. Although able to tolerate and thrive with this variation, cats have a metabolic response to fatty acids that is relatively unknown. This study shows that dietary PUFA resulted in changing circulating concentrations of that specific PUFA. Increasing dietary eicosapentaenoic acid EPA and docosahexaenoic acid DHA (E&D) resulted in little change in total circulating PUFA as compared to increasing dietary arachidonic acid (ARA) which resulted in an increased concentration of total circulating PUFA. Cats responded to increased dietary E&D by reducing circulating cholesterol as compared to control fed cats. Increasing dietary PUFA also resulted in a decrease in circulating betaine, dimethylglycine and sarcosine in comparison to the cats consuming the control food at the end of the study. Changing dietary PUFA also changed circulating concentrations of gut microbial purification postbiotics. Increasing dietary ARA resulted in an increased concentration of indoleacetate, indolepropionate and indoleacetylglutamine in comparison to cats fed foods enhanced with increased E&D. Increasing E&D resulted in a decreased concentration of 4-ethylphenylsulfate, 3-methyl catechol sulfate and 4-vinylphenol sulfate at the end of the feeding period as compared to cats fed increased ARA or fed the unsupplemented control food. These changes suggest that support of single carbon metabolism would benefit cats with increasing dietary PUFA, that increasing E&D beneficially lowered cholesterol and that dietary PUFA influenced gut microbes resulting in changes in their postbiotics. ABSTRACT: There is a normal variation of polyunsaturated fatty acids (PUFA) in the foods consumed both by the domestic cat and wild felines. This variation may lead to specific changes in metabolites and circulating fatty acids that influence health and response to disease. Therefore, in order to evaluate the response to these changes in dietary PUFA three foods were formulated: a complete and balanced control food (COF) with no enhanced source of added PUFA (ARA = 0.08%, EPA & DHA = 0.01%), Test food 1 (E&DF) like the COF with added eicosapentaenoic acid EPA and docosahexaenoic acid DHA (E&D = 0.36%)) from menhaden fish oil, and Test Food 2 (ARAF) like the COF with added arachidonic acid (ARA = 0.16%) from liver. All test foods had similar protein concentrations and similar vitamin and mineral concentrations while the PUFA supplemented foods had slightly higher fat concentrations. Cats (n = 36) were fed a pre-trial food for 28 days and then assigned to a group fed either the control, E&DF or ARAF for 56 days (12 cats per group). Blood samples were drawn and serum analyzed for fatty acids, albumin, urea, creatinine, cholesterol and triglycerides at the beginning of the study and after consuming the test foods for 28 and 56 days. Plasma was similarly analyzed for metabolomics. Increasing dietary E&D resulted in reduced cholesterol, betaine, dimethyl glycine, sarcosine and 4-ethylphenylsulfate. Increasing dietary ARA resulted in reduced betaine, dimethyl glycine and sarcosine and an increased concentration of indoleacetate, indolepropionate and indoleacetylglutamine. These data suggest a benefit of dietary single carbon metabolism support for cats supplemented with ARA or E&D. Moreover, the reduction in circulating cholesterol and triglycerides through dietary E&D supplementation could benefit cats with hyperlipidemia. Further research into the interrelationship between dietary PUFA and the gut microbe will benefit from the data showing that ARA increased specific positive postbiotics (i.e., indoleacetate, indolepropionate) while E&D supplementation showed the benefit of reducing some postbiotics which have been associated with reduced health (4-ethylphenylsulfate, 3-methyl catechol sulfate and 4-vinylphenol sulfate).